Celestine blue as a new indicator in electrochemical DNA biosensors

Hamidi‐Asl, Ezat; Raoof, Jahan Bakhsh; Meibodi, Abdolraoof Samadi; Bazgir, Zahra Haghnavaz

doi:10.1007/s11426-015-5445-3

Cited by 9 publications

(6 citation statements)

References 38 publications

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“…CB molecules bind to DNA molecules through electrostatic interactions. . After incubation of the modified electrode with CB, a pair of well defined reversible peaks was observed due to the electrochemical oxidation/reduction of the adsorbed CB (the inset of Figure .A).…”

Section: Resultssupporting

confidence: 83%

Highly Sensitive Electrochemical Hydrogen Peroxide Sensor Based on Iron Oxide‐Reduced Graphene Oxide‐Chitosan Modified with DNA‐Celestine Blue

et al. 2017

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The present study describes a novel and very sensitive electrochemical assay for determination of hydrogen peroxide (H2O2) based on synergistic effects of reduced graphene oxide‐ magnetic iron oxide nanocomposite (rGO‐Fe3O4) and celestine blue (CB) for electrochemical reduction of H2O2. rGO‐Fe3O4 nanocomposite was synthesized and characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X‐ray diffraction (XRD), electrochemical impedance spectroscopy and cyclic voltammetry. Chitosan (Chit) was used for immobilization of amino‐terminated single‐stranded DNA (ss‐DNA) molecules via a glutaraldehyde (GA) to the surface of rGO‐Fe3O4. The MTT (3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) results confirmed the biocompatibility of nanocomposite. Experimental parameters affecting the ss‐DNA molecules immobilization were optimized. Finally, by accumulation of the CB on the surface of the rGO‐Fe3O4‐Chit/ssDNA, very sensitive amperometric H2O2 sensor was fabricated. The electrocatalytic activity of the rGO‐Fe3O4‐Chit/DNA‐CB electrode toward H2O2 reduction was found to be very efficient, yielding very low detection limit (DL) of 42 nM and a sensitivity of 8.51 μA/μM. Result shows that complex matrices of the human serum samples did not interfere with the fabricated sensor. The developed sensor provided significant advantages in terms of low detection limit, high stability and good reproducibility for detection of H2O2 in comparison with recently reported electrochemical H2O2 sensors.

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Section: Resultssupporting

confidence: 83%

Highly Sensitive Electrochemical Hydrogen Peroxide Sensor Based on Iron Oxide‐Reduced Graphene Oxide‐Chitosan Modified with DNA‐Celestine Blue

et al. 2017

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“…The results exhibited that current signal of S2 was not affected by the mismatched sequences, indicating the sensor could perfectly distinguish the target from various interference sequences ( Figure 5 C). Compared with the Bazgir’s work [ 19 ], our further study in the sequence selectivity and anti-interference experiments showed its great potential in clinical diagnosis.…”

Section: Resultsmentioning

confidence: 75%

“…So it can be used as a promising hybridization indicator via binding to nucleic acids by electrostatic attraction, which can be employed in electrochemical DNA biosensors. Bazgir et al [ 19 ] designed the CB-modified carbon paste electrode and investigated the interaction mode between CB and nucleic acid through spectroscopic and electrochemical approaches. The detection limit of this approach was as low as 0.48 nM.…”

Section: Introductionmentioning

confidence: 99%

Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene

Chen

et al. 2022

Biosensors

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A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with no need for other substances for electrode or DNA functionalization. The interaction mode between CB and DNA was studied by electrochemical and spectroscopic approaches, illustrating that the possible mode was intercalation with a binding number of 2 and a binding constant β of 1012.87. Moreover, the label-free electrochemical DNA biosensor exhibited a good linear relationship toward the target gene in a range from 1.00 nM to 50.00 nM with a detection limit as low as 0.046 nM using 3σ estimating system. This facile and low-cost electrochemical method realized the rapid detection and accurate quantification of the target sequence in complicated serum samples, endowing its promising potential in the diagnosis and monitoring of genetic diseases.

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“…Ketamine abuse results a state of "dreamy intoxication", blurred vision and out of body experiences [7][8][9][10][11][12]. Recently, interaction of DNA and drugs has been a growing attractive topic in the electrochemical investigations [13][14][15][16]. For small molecules such as drugs, mechanism of interaction as well as evaluation of binding constant can be investigated by electrochemical studies.…”

Section: Introductionmentioning

confidence: 99%

A genosensor based on CPE for study the interaction between ketamine as an anesthesia drug with DNA

Asghary

Raoof

Ojani

et al. 2015

International Journal of Biological Macromolecules

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Celestine blue as a new indicator in electrochemical DNA biosensors

Cited by 9 publications

References 38 publications

Highly Sensitive Electrochemical Hydrogen Peroxide Sensor Based on Iron Oxide‐Reduced Graphene Oxide‐Chitosan Modified with DNA‐Celestine Blue

Highly Sensitive Electrochemical Hydrogen Peroxide Sensor Based on Iron Oxide‐Reduced Graphene Oxide‐Chitosan Modified with DNA‐Celestine Blue

Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene

A genosensor based on CPE for study the interaction between ketamine as an anesthesia drug with DNA

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