Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene

Abstract

A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with no need for other substances for electrode or DNA functionalization. The interaction mode between CB and DNA was studied by electrochemical and spectroscopic approaches, illustrating that the possible mode was intercalation with a binding number of 2 and a binding constant β of 1012.87. Moreover, the label-free electrochemical DNA biosensor exhibited a good linear relationship toward the target gene in a range from 1.00 nM to 50.00 nM with a detection limit as low as 0.046 nM using 3σ estimating system. This facile and low-cost electrochemical method realized the rapid detection and accurate quantification of the target sequence in complicated serum samples, endowing its promising potential in the diagnosis and monitoring of genetic diseases.

Optofluidic Sensor Based on Polymer Optical Microresonators for the Specific, Sensitive and Fast Detection of Chemical and Biochemical Species

“…These performances seem to be modest as compared to those obtained using other sensing for the detection of DNA strands, such as quart microbalance, electrochemistry or SPR, as reported in References [6][7][8][9], or FRET-based fluorescence, as reported in Reference [42] for 11 DNA base pairs, for which a detection limit of 50 fM has been evidenced.…”

“…For these reasons, different label-free detection methods have been proposed, some of them currently being developed for commercial use. Various physical phenomena may be involved in such recognition processes: mass variation using quartz microbalance [6], electrochemical sensors [7,8], plasmon resonance-based sensors [9,10], photonic crystals [11], optical fibers [12] and microresonators [13,14].…”

Disposable Voltammetric Determination of Celestine Blue at a Pencil Graphite Electrode

“…As a result, the widespread use of dyestuffs induced soil and water pollution [1][2][3][4]. The CelsB which is soluble in water and classified as an essential oxyazine group dye, shows good mediator properties due to its high electron transfer properties (Figure 1) [5]. The CelsB has a non-complex structure, and it is structurally positively charged property [6].…”

“…1 m.gunes@outlook.com (Corresponding Author) Figure 1. Structure of molecular CelsB [5] Electrochemical methods are still attractive due to their cheapness, high sensitivity, mobility, selectivity, speedy response, easy instrumentation, ability to be miniaturized properties [12,13]. Various carbon-based electrodes, such as PGE, CPE, and GCE have been widely used in the design of electrochemical sensors [14].…”

Cr-MOF-Based Electrochemical Sensor for the Detection of P-Nitrophenol

“…Compared with other traditional techniques, electrochemical sensing methods have the advantages of good selectivity, high sensitivity, simple operation, and fast response time [ 7 , 8 , 9 ]. The detection performance of an electrochemical sensor depends on the chemical properties of the modified materials used in the working electrodes [ 10 , 11 ].…”