A genosensor based on CPE for study the interaction between ketamine as an anesthesia drug with DNA

Asghary, Maryam; Raoof, Jahan Bakhsh; Ojani, Reza; Hamidi‐Asl, Ezat

doi:10.1016/j.ijbiomac.2015.07.019

Cited by 24 publications

(11 citation statements)

References 51 publications

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“…The PGEs provide more rapid and practical detection process comparison to other electrodes, such as carbon paste electrode , glassy carbon electrode , gold electrode . In comparison to the results presented in earlier studies, our electrochemical biosensor platform showed some advantages such as label‐free, cost‐effective, easy‐to‐handle, single‐use and portable.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresis

Kanat

Ekşin

Karacicek³

et al. 2018

Electroanalysis

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Anticancer drug Dacarbazine (DCB) has been used for the treatment for metastatic malignant melanoma. In our study, the biomolecular interaction between DCB and DNA was investigated electrochemically for the first time using single‐walled carbon nanotube (SWCNT) modified disposable pencil graphite electrode (PGEs). The SWCNT modified PGEs were optimized for advanced DNA sensing based on the response related to changes at the guanine oxidation signal. The oxidation signals of DCB and guanine were respectively measured before/after surface confined interaction process using differantial pulse voltammetry (DPV) in the same voltammetric scale. The experimental parameters, such as DCB concentration and interaction time of DCB with fsDNA were studied. The detection of interaction between DCB and nucleic acids was also studied via electrochemical impedance spectroscopy (EIS) and gel electrophoresis. In addition, the effect of time‐dependent manner from 1 min to 10 min on the interaction of DCB with nucleic acids was explored upon to the changes at guanine signal of fsDNA as well as PCR samples by DPV technique.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresis

Kanat

Ekşin

Karacicek³

et al. 2018

Electroanalysis

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Section: Resultsmentioning

confidence: 99%

“…None of these compounds produced peaks in the CV scan or caused any change in the flunitrazepam peak heights (Figure S2, Supplementary Materials). Indeed, GHB is known to be oxidized only under very specific conditions [18] while both ketamine [20] and scopolamine [19] are oxidized at more positive potentials. The limits of detection obtained with the proposed sensor were in the range 0.57-4.6 mg L −1 , depending on the type of sample and the dilution level.…”

Section: Interference Studymentioning

confidence: 99%

Rapid Drop-Volume Electrochemical Detection of the “Date Rape” Drug Flunitrazepam in Spirits Using a Screen-Printed Sensor in a Dry-Reagent Format

Papadopoulos

Diamanteas

Economou

et al. 2020

Sensors

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Flunitrazepam is an extremely potent benzodiazepine sedative which is associated with “drug-facilitated sexual assault” when administered within an alcoholic drink. This work describes a simple electrochemical method for on-site rapid detection of flunitrazepam in untreated spirits (whiskey, vodka and gin) using a single-use screen-printed sensor (featuring graphite working and auxiliary electrodes and an Ag/AgCl reference electrode) in a dry reagent format. Analysis was performed by placing a drop of sample on the sensor, which was previously coated with dry KCl, and recording selected reduction/oxidation peaks of the target compound in a cyclic voltammetry scan. The limit of quantification of flunitrazepam was at the sub-mg L−1 range. The between-sensor % relative standard deviation of the analytically useful reduction peak in a solution containing 11.4 mg L−1 flunitrazepam was 9.8% (n = 5). Quantification was performed using calibration curves constructed from pooled samples spiked with flunitrazepam with relative errors <15%. The main advantages of the methodology are that it involves no sample pretreatment (such as deoxygenation, extraction or reagent(s) addition) and requires only drop-sized volumes of the sample, thus facilitating rapid on-site screening using portable equipment.

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“…∆ G is defined in eqn (4) as:

italicΔG = italicΔH - italicTΔS

where ∆ G is Gibbs free energy, ∆ H is enthalpy change, ∆ S is entropy change and T is the temperature. Signs of ∆ H and ∆ S can reflect the binding mechanism between a small molecule and DNA: hydrophobic forces are dominant when ∆ H > 0 and ∆ S > 0; van der Waals interaction and hydrogen bonds are the main interactions contributing to the binding when ∆ H < 0 and ∆ S < 0; and electrostatic interactions are dominant when ∆ H < 0 and ∆ S > 0 eqn (5):

ln K_{a} = - \frac{ΔH}{RT} + \frac{ΔS}{R}

where K a is the binding constant at the corresponding temperature T , and R is the gas constant. As listed in Table , negative values of ∆ G and ∆ H and positive values of ∆ S were obtained, and this finding indicated that the binding of cephalosporin and DNA is a spontaneous process driven by both enthalpy and entropy, and electrostatic interactions are the main binding force .…”

Section: Resultsmentioning

confidence: 99%

Comparative study of two cephalosporin antibiotics binding to calf thymus DNA by multispectroscopy, electrochemistry, and molecular docking

et al. 2019

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The over-use of antibiotics has caused a number of problems such as contamination of antibiotic residues and virus resistance, and therefore has attracted global attention. In this study, spectroscopic techniques and molecular docking were employed to predict conformational changes and binding interaction between two cephalosporins (cefaclor and cefixime) and calf thymus DNA (ctDNA). Fluorescence and UV-vis spectra suggested that static quenching was predominant and cephalosporin bound to the groove region of ctDNA. Binding parameters calculated by the Stern-Volmer and Scatchard equations showed that cephalosporin bound to ctDNA with a binding affinity in the order of 10 3 L mol −1 . Thermodynamic parameters further indicated that the reaction was a spontaneous process driven by enthalpy and entropy, and that the main binding force was an electrostatic force. The effects of iodide, denaturant, thermal denaturation and pH on a cephalosporin-Hoechst-DNA complex were also studied, and the results confirmed that cephalosporin bound to the groove area of DNA.Finally, these results were further confirmed by molecular docking and electrochemical studies.

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A genosensor based on CPE for study the interaction between ketamine as an anesthesia drug with DNA

Cited by 24 publications

References 51 publications

Electrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresis

Electrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresis

Rapid Drop-Volume Electrochemical Detection of the “Date Rape” Drug Flunitrazepam in Spirits Using a Screen-Printed Sensor in a Dry-Reagent Format

Comparative study of two cephalosporin antibiotics binding to calf thymus DNA by multispectroscopy, electrochemistry, and molecular docking

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