Electrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresis

Kanat, Erkin; Ekşin, Ece; Karacicek, Bilge; Erac, Yasemin; Erdem, Arzum

doi:10.1002/elan.201800064

Cited by 15 publications

(9 citation statements)

References 37 publications

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“…Thus, the oxidation signals of both DCB and guanine can be measured using differential pulse voltammetry. The voltammetric results reported in [78] were found in good agreement with gel electrophoresis analysis. A decrease of ethidium bromide (EtBr) luminescence intensity was observed in contrast to the control in PCR samples while increasing the incubation time of DCB.…”

Section: Electrochemical Nucleic Acid-based Sensorssupporting

confidence: 73%

“…The DNA/GO/GCE sensor showed a detection limit of 7.6 nM and was tested for MTX determination in spiked urine and blood serum samples. The biomolecular interactions between DNA and the anticancer drug dacarbazine (DCB), a molecule frequently used for the treatment of metastatic malignant melanoma, was investigated using a single-walled carbon nanotube modified disposable pencil graphite electrode [78]. DCB acts as alkylating agent and its binding to DNA strands affects the intrinsic electrochemical activity of DNA; moreover, it is an electroactive molecule itself.…”

Section: Electrochemical Nucleic Acid-based Sensorsmentioning

confidence: 99%

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Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Tadini‐Buoninsegni

Palchetti

2020

Sensors

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Cancer is a multifactorial family of diseases that is still a leading cause of death worldwide. More than 100 different types of cancer affecting over 60 human organs are known. Chemotherapy plays a central role for treating cancer. The development of new anticancer drugs or new uses for existing drugs is an exciting and increasing research area. This is particularly important since drug resistance and side effects can limit the efficacy of the chemotherapy. Thus, there is a need for multiplexed, cost-effective, rapid, and novel screening methods that can help to elucidate the mechanism of the action of anticancer drugs and the identification of novel drug candidates. This review focuses on different label-free bioelectrochemical approaches, in particular, impedance-based methods, the solid supported membranes technique, and the DNA-based electrochemical sensor, that can be used to evaluate the effects of anticancer drugs on nucleic acids, membrane transporters, and living cells. Some relevant examples of anticancer drug interactions are presented which demonstrate the usefulness of such methods for the characterization of the mechanism of action of anticancer drugs that are targeted against various biomolecules.

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Section: Electrochemical Nucleic Acid-based Sensorssupporting

confidence: 73%

Section: Electrochemical Nucleic Acid-based Sensorsmentioning

confidence: 99%

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Tadini‐Buoninsegni

Palchetti

2020

Sensors

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“…As a conclusion, single use pencil graphite electrodes utilized herein for monitoring biorecognition processes owing to its many advantages; such as cost effectiveness, rapid and sensitive detection with good repeatability of drug‐DNA interaction in comparison to earlier studies performed by using other electrodes, such as GCE, HMDE and CPE . The electrochemical monitoring of the surface‐confined interaction between DNA and DNA‐targeted molecules would be valuable in the rational design of the molecule‐specific electrochemical biosensor, and in the further development of novel drugs for the chemotherapy.…”

Section: Resultsmentioning

confidence: 88%

“…In our previous study, the biomolecular interaction between DTIC and fish sperm DNA was investigated electrochemically with singlewalled carbon nanotube (SWCNT) modified PGEs. The detection of interaction between DTIC and PCR samples was also studied via EIS and gel electrophoresis [20].…”

Section: Introductionmentioning

confidence: 99%

Voltammetric and Impedimetric Detection of Interaction Between Dacarbazine and Nucleic Acids

2019

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Dacarbazine (DTIC) is a chemotherapy drug that is used for the treatment of Hodgkin's lymphoma, malignant melanoma, childhood solid tumors and soft tissue sarcoma. The surface confined interaction between DTIC and nucleic acids was investigated for the first time in this study by using both differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) in combination with disposable pencil graphite electrodes. The oxidation signals of DTIC and guanine were measured before and after interaction process using DPV technique. The interaction of DTIC with nucleic acids; poly[A], poly[G], or double stranded of poly[A]‐poly[T] and poly[G]‐poly[C] was also examined using DPV. Furthermore, EIS technique was utilized for detection of the interaction between DTIC and nucleic acids; ssDNA/dsDNA, poly[A], poly[G], or double stranded poly[A]‐poly[T] and poly[G]‐poly[C].

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“…The analysis in DPV is quite similar to CV; changes in the current peak and potential are parameters that are associated with the event. Examples of drugs that have been tested to interact with DNA using DPV are topotecan, codeine, gemcitabine, dextromethorphan and dacarbazine .…”

Section: Electroanalytical Techniques Used To Evidence Drug‐dna Intermentioning

confidence: 99%

Nanomaterial‐based Sensors for the Study of DNA Interaction with Drugs

2019

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The interaction of drugs with DNA has been searched thoroughly giving rise to an endless number of findings of undoubted importance, such as a prompt alert to harmful substances, ability to explain most of the biological mechanisms, or provision of important clues in targeted development of novel chemotherapeutics. The existence of some drugs that induce oxidative damage is an increasing point of concern as they can cause cellular death, aging, and are closely related to the development of many diseases. Because of a direct correlation between the response, strength/ nature of the interaction and the pharmaceutical action of DNA‐targeted drugs, the electrochemical analysis is based on the signals of DNA before and after the interaction with the DNA‐targeted drug. Nowadays, nanoscale materials are used extensively for offering fascinating characteristics that can be used in designing new strategies for drug‐DNA interaction detection. This work presents a review of nanomaterials (NMs) for the study of drug‐nucleic acid interaction. We summarize types of drug‐DNA interactions, electroanalytical techniques for evidencing these interactions and quantification of drug and/or DNA monitoring.

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Electrochemical Detection of Interaction between Dacarbazine and Nucleic Acids in Comparison to Agarose Gel Electrophoresis

Cited by 15 publications

References 37 publications

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Voltammetric and Impedimetric Detection of Interaction Between Dacarbazine and Nucleic Acids

Nanomaterial‐based Sensors for the Study of DNA Interaction with Drugs

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