Ceftiofur distribution in plasma and joint fluid following regional limb injection in cattle

Abstract

The objective of this study was to evaluate the efficacy of regional intravenous (i.v.) injection of ceftiofur in delivery of this drug to joint fluid and plasma in a limb distal to a tourniquet in five, healthy, adult, mixed breed beef cattle. A tourniquet was positioned in the mid-metacarpal region, and 500 mg of ceftiofur was administered through a catheter in the dorsal common digital vein (DCDV). Plasma samples were collected from the catheter at 15, 30 and 45 min postinjection, and from the abaxial proper palmar vein (APPV) at 15 min postinjection. Synovial fluid was collected from the metacarpal phalangeal joint at 45 min postinjection. Ceftiofur concentrations were estimated in plasma and synovial fluid using high-pressure liquid chromatography (HPLC) and a microbiological assay utilizing Pasteurella haemolytica as the test organism. Both assays indicated highest plasma concentrations of ceftiofur at 15 min, with the concentrations declining with time. Concentrations of ceftiofur in plasma obtained from the DCDV were not significantly different from APPV levels, indicating rapid distribution of ceftiofur within the limb. Microbiological assay always demonstrated higher concentrations of ceftiofur compared with HPLC assay, because the former probably also detected the active metabolites of ceftiofur as well as the parent compound. At 45 min, ceftiofur concentrations determined by HPLC were 251+/-97 and 15+/-5 microg/mL in plasma and synovial fluid, respectively. Regional intravenous injection appears to be a feasible technique to produce rapid distribution of ceftiofur within the limb well above therapeutic concentrations.

On-line coupling of solid-phase extraction and capillary electrophoresis for the determination of cefoperazone and ceftiofur in plasma

“…This versatile technique has been commonly used to analyze cephalosporin residues in biological samples such as milk [2,3], plasma [4], serum [1,2,5], cerebrospinal fluid [5], urine [1,5] and synovial fluid [4]. Some papers have been published the determination of cefoperazone [6] or ceftiofur [4,[7][8][9] in plasma by HPLC and two different plasma pretreatments were used: a simple deproteinization, (using organic solvent or acidic media) [6,7], and a derivatization step followed by SPE [4,8,9]. Detection limits ranged from 1000 [6,7] to 150 ng ml −1 [4,9], for the deproteinization and the derivatization pretreatment, respectively.…”

Treatment of a Sole Abscess in an Asian Elephant (Elephas Maximus) Using Regional Digital Intravenous Perfusion

“…perfusion method used in domestic horses, cattle, and rabbits. 1,2,6,11,12 Although a specialized pneumatic tourniquet was not available for the Asian cow in this study, the successful outcome suggests that the rope tourniquet was satisfactory. Venous access was more difficult in this Asian elephant, compared with the African elephant in which the procedure was first described.…”

“…perfusion technique was originally described for one-time therapy of osteomyelitis in domestic animals, including cows and rabbits. 1,2,6,11,12 In domestic animals, it involves the injection, through a catheter, of local anesthetic and antibiotics into a distended vein of a limb, distal to a tourniquet. In this manner, therapeutic antibiotic concentrations are reached in the tissues distal to the tourniquet using a relatively small dose of an- tibiotic.…”

Determinação de tetraciclina em líquido sinovial de vacas com doença podal

“…Navarre et al (1999) também utilizou esta técnica na extração de ceftiofur por CLAE em plasma. Tal procedimento igualmente apresentou sucesso na matriz biológica estudada constituindo-se de uma téc-nica simples, rápida e econômica.…”

“…As opções de tratamento para tais patologias são limitadas e agravam-se ainda mais quando o processo infeccioso atinge os ossos e as articulações (Clarkson et al, 1996;Desrochers et al, 1995;Kofler, 1995;Navarre et al, 1999;Stanek, 1994).…”