Ceftiofur Distribution in Plasma and Tissues Following Subcutaneously Administration in Ducks

Abstract

ABSTRACT. A study was performed to determine the residues in blood and edible tissues of healthy ducks (25 days old, mean body weight 1.0 ± 0.13 kg) after subcutaneous administration of ceftiofur sodium at a dose rate of 2 mg/kg body weight (Group I) and 4 mg/kg body weight (Group II). Blood, muscle, liver, kidney, and fat samples were collected from all of ducks on the 1st, 2nd, 3rd, 4th, and 5th day after treatment of drug, and ceftiofur was analyzed with a high-performance liquid chromatography (HPLC) assay with results reported as ceftiofur-free acid equivalent (CFAE). To study the spiked recovery, blank plasma and tissues were spiked with two different concentrations of ceftiofur sodium (0.1, 0.5 µg/g). Average recovery values for all samples ranged from 70.3 to 87.3%. In the group I, desfuroylceftiofur acetamide (DCA) was not detected in all of plasma, muscle, liver, and fat tissues on the 1st day after treatment. But, kidney samples on the 1st day were detected DCA (0.059 ± 0.01 µg CFAE/g tissue). On the 2nd day of post-treatment, the concentrations of DCA in all tissues were lower than the detection limit, 0.05 µg CFAE /g tissue. In the group II on the 1st day after treatment, the concentration of DCA was 0.124 ± 0.06 µg CFAE/g tissue, 0.103 ± 0.03 µg CFAE/g tissue, and 0.071 ± 0.010 µg CFAE/g tissue in plasma, kidney, and muscle samples, respectively. On the 2nd day after treatment of ceftiofur, the concentrations of DCA in all tissues were lower than 0.05 µg CFAE/g tissue. According to our results, the concentrations of DCA on the 1st day after treatment with 2 mg/ kg body weight were below 0.05 µg CFAE/g tissue equivalent in all tissues except for kidney. On the 2nd day after administration at the dose of 4 mg/kg body weight, no DCA was also detected in all of the tissues although DCA was detected in all samples on the 1st day.

Residues of Ceftiofur Sodium in Rabbit Tissues

“…These results are slightly agreed with that reported by El-Sayed et al [11] who mentioned that kidney and liver have the highest drug concentrations, while the lowest was in thigh muscle at the 1 st day after stoppage of intramuscular administration of ceftiofur at a dose of 10 mg/kg BW for 5 days. Also, our results are in agreement with Chung et al, [6] who stated that kidney contained the highest drug residue concentration. Also, they reported that the drug residue concentration in muscle (0.071±0.01 μg/g) was more than in liver (0.066±0.01 μg/g) at the 1 st day post-drug medication (4 mg/kg BW subcutaneously for 1 day) which was disagreed with our result.…”

“…Extraction was carried out according to Chung et al [6]. Frozen samples were thawed at room temperature (23°C).…”

Assessment of liquid chromatography–tandem mass spectrometry approaches for the analysis of ceftiofur metabolites in poultry muscle

“…Levels in the thigh muscle were lower, whereas the highest concentrations were detected in the kidneys. This is in agreement with previous findings after intramuscular treatment of swine Beconi-Barker et al 1997) and subcutaneous injection of ducks (Chung et al 2007). It is concluded that, from an analytical point of view, poultry kidney is potentially better suited for detecting off-label use of ceftiofur.…”

Pharmacokinetics of Ceftiofur Sodium in Black-bone Silky Fowl after One Single Intravenous and Intramuscular Injection

“…The pharmacokinetics profiles of ceftiofur have been determined in different avian species, such as chickens (Tell et al, 1998), ducks (Chung et al, 2007;Hope et al, 2012), geese (Chen et al, 2014) and turkeys (Tell et al, 1998). Ceftiofur has some similar pharmacokinetics profiles in these birds, such as a relatively poor distribution, but a quick and complete transformation to its active metabolites, desfuroylceftiofur (DCE) and desfuroylceftiofur conjugates (DCEC).…”