cDNA cloning and expression analysis of myostatin/GDF11 in shrimp, Litopenaeus vannamei

Qian, Zhaoying; Xiao, Mei; Wang, Xian-Zong; He, Shulin; Liu, Yongjie; Hou, Fujun; Qiao, Li; Liu, Xiaolin

doi:10.1016/j.cbpa.2013.02.001

Cited by 36 publications

(20 citation statements)

References 46 publications

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“…Since then, this gene has attracted increasing interest in crustacean research. Recently, MSTN was characterized in crustaceans such as Eriocheir sinensis (Kim et al, ), P. japonica (Kim et al, ) , P. monodon (De Santis et al, ), and L. vannamei (Qian et al, ). The earlier reported Mr MSTN gene consists of 1,619 base pairs (bp), with an open reading frame of 945 bp that encodes 315 amino acids (Sarasvathi, Bhassu, Maningas, & Othman, ).…”

Section: Discussionmentioning

confidence: 99%

“…dsRNA: double-stranded RNA; GFP: greed florescent protein; MSTN: myostatin4 | DISCUSSIONThe first MSTN reported in invertebrates, and specifically in crustaceans, was from G. lateralis(Covi et al, 2008).Since then, this gene has attracted increasing interest in crustacean research. Recently, MSTN was characterized in crustaceans such as Eriocheir sinensis(Kim et al, 2009), P. japonica, P. monodon(De Santis et al, 2011), and L. vannamei(Qian et al, 2013). The earlier reported MrMSTN gene consists of 1,619 base pairs (bp), with…”

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confidence: 99%

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Enhanced muscle regeneration in freshwater prawn Macrobrachium rosenbergii achieved through in vivo silencing of the myostatin gene

Easwvaran

Bhassu

Maningas

et al. 2019

J World Aquaculture Soc

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Myostatin (MSTN) is an interesting negative growth‐regulating gene that has been well characterized in vertebrates but scantly described in invertebrates. The current study focuses on the downregulation of the MrMSTN gene and subsequently records any histological changes for giant freshwater prawn, Macrobrachium rosenbergii (Mr). In addition, the study also deals with the MrMSTN gene's influence on other growth‐related genes, which include myosin heavy chain, dystrophin‐dystroglycoprotein complex, tropomyosin, farnesoic acid o‐methyl transferase, arginine kinase, cyclophilin, and acyl CoA desaturase. The preliminary histological analysis following MrMSTN silencing favors muscle regeneration, which supports its functional role as a negative growth regulator and its significant effect on the expression of other growth‐related genes. Overall, our results show that the MrMSTN gene could therefore be a potential target for gene manipulation aimed at enhancing the growth and muscle development of M. rosenbergii, which could be beneficial in increasing the total mass production in the postlarva phase at the hatchery level.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Enhanced muscle regeneration in freshwater prawn Macrobrachium rosenbergii achieved through in vivo silencing of the myostatin gene

Easwvaran

Bhassu

Maningas

et al. 2019

J World Aquaculture Soc

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“…Nevertheless, in other vertebrates such as fishes, myostatin expression has been observed in several tissues/organs in addition to skeletal muscle, such as the brain, eyes, pancreas, gills, gonads, heart, intestine, kidney, liver, skin, spleen, and stomach (Maccatrozzo et al, ; Radaelli et al, ). Similarly, in invertebrates as crustaceans, myostatin expression has also been observed in several organs/tissues (Covi et al, ; MacLea et al, ; Qian et al, ). Additionally, in bivalve mollusks, specifically in scallops, such as Chlamys farreri and Argopecten irradians (Kim et al, ; Hu et al, ; Guo et al, ), the myostatin organ/tissue expression has been demonstrated, showing higher expression levels in the adductor muscle, followed by mantle and gills, with lower expression levels in kidney, digestive gland, gonad, smooth muscle, and hemocytes.…”

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confidence: 93%

Expression of the myostatin gene in the adductor muscle of the Pacific lion‐paw scallop Nodipecten subnodosus in association with growth and environmental conditions

et al. 2015

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The cDNA sequence of the myostatin gene in the Pacific lion-paw Nodipecten subnodosus (Ns-mstn) was characterized, and the temporal expression during grow-out was analyzed for the first time in a scallop. Ns-mstn encodes a 459-amino-acid protein in which two propeptide proteolytic sites were identified, the previously recognized (RSKR) and a second one at position 266-269 aa (RRKR). The alternative furin cleavage site could be related with post-translational processing, or it could be a tissue-specific mechanism for signaling activity. The Ns-mstn transcript was located by in situ hybridization in sarcomeres and around the nucleus of muscle fibers. The temporal expression analysis by qPCR in the adductor muscle showed that Ns-mstn expression was significantly different (P < 0.05) between months during the grow-out period, increasing largely during the summer months when both biomass and muscle weight did not increase or even decreased; muscle fiber size and number were found to decrease significantly. Exogenous and endogenous factors such as high temperature and low food availability, as well as gametogenesis and reproduction, can be associated with the growth pattern and Ns-mstn expression changes. Our results indicate that MSTN is involved in adductor muscle growth regulation in N. subnodosus as it occurs in vertebrate skeletal muscle although Ns-mstn expression in non-muscle organs/tissues suggests additional functions.

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“…As for crustaceans, some members of TGF-b family have been reported, such as growth differentiation factor-11 (GDF-11) in the white shrimp, Litopenaeus vannamei and freshwater shrimp, Macrobrachium nipponense (Qian et al, 2013;Lee et al, 2015;Shen et al, 2015), BMP-encoding gene (glassbottom boat, gbb) in the water flea, Daphnia pulex (Fritsch et al, 2010). However, whether BMPs have relation to reproduction in crustaceans is still unknown.…”

Section: Introductionmentioning

confidence: 98%

A bone morphogenetic protein ligand and receptors in mud crab: A potential role in the ovarian development

Shu

Yang

Huang

et al. 2016

Molecular and Cellular Endocrinology

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cDNA cloning and expression analysis of myostatin/GDF11 in shrimp, Litopenaeus vannamei

Cited by 36 publications

References 46 publications

Enhanced muscle regeneration in freshwater prawn Macrobrachium rosenbergii achieved through in vivo silencing of the myostatin gene

Enhanced muscle regeneration in freshwater prawn Macrobrachium rosenbergii achieved through in vivo silencing of the myostatin gene

Expression of the myostatin gene in the adductor muscle of the Pacific lion‐paw scallop Nodipecten subnodosus in association with growth and environmental conditions

A bone morphogenetic protein ligand and receptors in mud crab: A potential role in the ovarian development

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