Expression of the myostatin gene in the adductor muscle of the Pacific lion‐paw scallop Nodipecten subnodosus in association with growth and environmental conditions

Abstract

The cDNA sequence of the myostatin gene in the Pacific lion-paw Nodipecten subnodosus (Ns-mstn) was characterized, and the temporal expression during grow-out was analyzed for the first time in a scallop. Ns-mstn encodes a 459-amino-acid protein in which two propeptide proteolytic sites were identified, the previously recognized (RSKR) and a second one at position 266-269 aa (RRKR). The alternative furin cleavage site could be related with post-translational processing, or it could be a tissue-specific mechanism for signaling activity. The Ns-mstn transcript was located by in situ hybridization in sarcomeres and around the nucleus of muscle fibers. The temporal expression analysis by qPCR in the adductor muscle showed that Ns-mstn expression was significantly different (P < 0.05) between months during the grow-out period, increasing largely during the summer months when both biomass and muscle weight did not increase or even decreased; muscle fiber size and number were found to decrease significantly. Exogenous and endogenous factors such as high temperature and low food availability, as well as gametogenesis and reproduction, can be associated with the growth pattern and Ns-mstn expression changes. Our results indicate that MSTN is involved in adductor muscle growth regulation in N. subnodosus as it occurs in vertebrate skeletal muscle although Ns-mstn expression in non-muscle organs/tissues suggests additional functions.

Functional characteristic and differential expression ofmyostatininChlamys nobilis

“…Tissue-specific expression profile indicated that MSTN mRNA levels were found to be highest in adductor muscle, which was in keeping with the result of C. farreri, A. purpuratus, A. irradians, N. subnodosus and mammalian MSTN (Hu et al 2010;Guo et al 2012;Morales-Collio et al 2014;Morelos et al 2015). It was suggested that a highly conservation of role in muscle development.…”

Transcriptome analysis of Catarina scallop (Argopecten ventricosus) juveniles treated with highly-diluted immunomodulatory compounds reveals activation of non-self-recognition system

“…For transcriptomic analysis, two pools (~100 mg each) were used per treatment ( Fig 1A). The RNA extraction was assessed following the methodology previously described for the scallop Nodipecten subnodosus RNA extraction [35], using the TriPure reagent (Roche Diagnostics, Indianapolis, IN, USA), followed by ethanol/chloroform purification and DNAse cleaning. Samples were determined for RNA concentration using a NanoDrop 2000/ 2000c1 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and electrophoresis was performed to evaluate RNA quality.…”

“…Good quality RNA from a total of 20 Pools (two per treatment per tissue) were sent to the Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO, Laboratory of Genomics Services, CINVESTAV, Campus Irapuato, GTO, MX) to create and sequence the RNA-seq libraries using the Illumina Next-Seq1 (San Diego, CA, USA). For RT-qPCR analysis, the cDNA synthesis was performed following the methodology previously reported to quantify gene relative expression levels in N. subnodosus [35].…”

Agricultural Homoeopathy: A New Insight into Organics

“…At the begining of frlowering, a sample was taken to perform the expression analysis of the 9-cis-epoxycarotenoid gene (PvNCDE1) [54]. Total RNA isolation, cDNA synthesis and real-time PCR amplification were performed following the methodology reported by Morelos et al [55], and the relative expression of PvNCDE1 was estimated following the model proposed by Hellemans et al [56]. One-way ANOVA followed by Tukey's exact test was performed using STATISTICA 8.0 (StatSoft, Inc.).…”