cDNA clones encoding immunoglobulin λ chains from rabbit expressing the phenotype c7

Abstract

A cDNA library derived from spleen cells of an unimmunized rabbit expressing the c7 phenotype of Ig lambda chains (c7+, c21-) was screened with V lambda or C lambda probes of a lambda light chain bearing c21 epitopes. The nucleotide sequences of three hybridizing clones were found to be identical within the V lambda, J lambda and C lambda regions. The V lambda region was 97% similar to that of the functional germ-line gene V lambda 2, and the C lambda region was identical to that of gene C lambda 6, recently identified. Gene C lambda 6 exhibited four codon differences when compared with gene C lambda 5, the latter encoding c21 epitopes. The data presented here and in the accompanying report (Jaton, J.-C. et al., Eur. J. Immunol. 1990, 20:2713) support the view that gene C lambda 6 encodes the C region of c7 lambda chains and that c7 and c21 markers designate two distinct isotypic forms of lambda chains. On the basis of comparative Southern blotting analyses and restriction maps of cloned genomic regions containing V lambda and C lambda genes, a scheme is proposed to account for the c7- and c21- phenotypes.

Accuracy and coverage assessment of Oryctolagus cuniculus (rabbit) genes encoding immunoglobulins in the whole genome sequence assembly (OryCun2.0) and localization of the IGH locus to chromosome 20

“…In addition to the initial 3 Mb gap, the scaffold containing the IGL genes consists of more than 100 contigs with gaps between them. Rabbit lambda constant region genes, IGLC5 paired with IGLJ5 and IGLC6 paired with IGLJ6 are known to be functional (Hayzer et al 1990; Jaton et al 1990). …”

“…See: http://imgt.org/IMGTrepertoire/index.php?section=LocusGenes&repertoire=genetable&species=rabbit&group=IGLC http://imgt.org/IMGTrepertoire/index.php?section=LocusGenes&repertoire=genetable&species=rabbit&group=IGLJ http://imgt.org/IMGTrepertoire/index.php?section=LocusGenes&repertoire=genetable&species=rabbit&group=IGLV IMGT lists position numbers for the contig NW_003159316.1. These are 3 Mb lower than position numbers on chromosome 21. b Lower quality alignments to both of M12388.1 and M127621.1 IGLC1 and IGLC2 respectively, are also found on chrUn0351 (NW_003159675.1), chrUn0545 (NW_003159869.1) and chrUn0895 (NW_003160219). c Rabbit lambda constant region genes, IGLC5 paired with IGLJ5*01 and IGLC6 paired with IGLJ6*01 are known to be functional (Hayzer et al 1990; Jaton et al 1990). …”

“… c Rabbit lambda constant region genes, IGLC5 paired with IGLJ5*01 and IGLC6 paired with IGLJ6*01 are known to be functional (Hayzer et al 1990; Jaton et al 1990). …”