cDNA-AFLP analysis of seed germination in Arabidopsis thaliana identifies transposons and new genomic sequences

Diego, Juana Gutiérrez de; Rodríguez, Fernando Mateos; Lorenzo, José Luis Rodríguez; Grappin, Philippe; Cervantés, Emilio

doi:10.1016/j.jplph.2005.04.032

Cited by 25 publications

(12 citation statements)

References 46 publications

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“…Of note, a number of the TDFs did not correspond to available sequences from plants based on the homology analysis by BLASTX/N. Similar cases have been reported for Arabidopsis, in which TDFs showed significant similarity with DNA or protein sequences from animal species (De Diego et al, 2006) or microorganisms (Mondego et al, 2003). Together, these TDFs and those with no BLAST hits may be regarded as newly identified genes expressed during flower development, and warrant further investigation.…”

Section: Discussionmentioning

confidence: 52%

cDNA-AFLP transcriptional profiling reveals genes expressed during flower development in Oncidium Milliongolds

Qian¹,

Gong²,

Wang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The flower developmental process, which is crucial to the whole lifecycle of higher plants, is influenced by both environmental and endogenous factors. The genus Oncidium is commercially important for cut flower and houseplant industry and is ideal for flower development studies. Using cDNA-amplified restriction fragment length polymorphism analysis, we profiled transcripts that are differentially expressed during flower development of Oncidium Milliongolds. A total of 15,960 transcript-derived fragments were generated, with 114 primer sets. Of these, 1248 were sequenced, producing 993 readable sequences. BLASTX/N analysis showed that 833 of the 993 transcripts showed homology to genes in the NCBI databases, exhibiting functions involved in various processes, such as signal transduction, energy conversion, metabolism, and gene expression regulation. The full-length mRNAs of SUCROSE SYNTHASE 1 (SUS1) and LEAFY (LFY) were cloned, and their expression patterns were characterized. The results showed that the expression levels of SUS1 and LFY were similar during flower development. To confirm the function of SUS1 in flower buds, 6304 X. Qian et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6303-6315 (2014) carbohydrate content and sucrose synthase activity were determined. The results showed that changes in sucrose content and sucrose synthase activity reflected SUS1 expression levels. Collectively, these results indicate that SUS1 influences flower development by regulating LFY expression levels through changing the sucrose content of flower buds.

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Section: Discussionmentioning

confidence: 52%

cDNA-AFLP transcriptional profiling reveals genes expressed during flower development in Oncidium Milliongolds

Qian¹,

Gong²,

Wang³

et al. 2014

Genet. Mol. Res.

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“…(58,59) Numerous gaps also still remain in the human genome, which are now known to contain genes. (60) It is likely that many of the missing genes reside in heterochromatin.…”

Section: More Ding Proteinsmentioning

confidence: 99%

The DING family of proteins: ubiquitous in eukaryotes, but where are the genes?

et al. 2009

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PstS and DING proteins are members of a superfamily of secreted, high-affinity phosphate-binding proteins. Whereas microbial PstS have a well-defined role in phosphate ABC transporters, the physiological function of DING proteins, named after their DINGGG N termini, still needs to be determined. PstS and DING proteins co-exist in some Pseudomonas strains, to which they confer a highly adhesive and virulent phenotype. More than 30 DING proteins have now been purified, mostly from eukaryotes. They are often associated with infections or with dysregulation of cell proliferation. Consequently, eukaryotic DING proteins could also be involved in cell-cell communication or adherence. The ubiquitous presence in eukaryotes of proteins structurally and functionally related to bacterial virulence factors is intriguing, as is the absence of eukaryotic genes encoding DING proteins in databases. DING proteins in eukaryotes could originate from unidentified commensal or symbiotic bacteria and could contribute to essential functions. Alternatively, DING proteins could be encoded by eukaryotic genes sharing special features that prevent their cloning. Both hypotheses are discussed.

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“…A Phb partial sequence from A. thaliana was obtained (de Diego et al, 2006). A cDNA containing the complete coding sequence corresponding to Phb encoded in clone K17E12 in chromosome 3 (At3g27280) was obtained, confirmed by sequencing, and used for the two-hybrid analysis.…”

Section: Cloning Of Phb Cdna Clonesmentioning

confidence: 99%

The prohibitin genes in Arabidopsis thaliana: Expression in seeds, hormonal regulation and possible role in cell cycle control during seed germination

Diego¹,

Rodríguez²,

Lorenzo³

et al. 2007

Journal of Plant Physiology

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cDNA-AFLP analysis of seed germination in Arabidopsis thaliana identifies transposons and new genomic sequences

Cited by 25 publications

References 46 publications

cDNA-AFLP transcriptional profiling reveals genes expressed during flower development in Oncidium Milliongolds

cDNA-AFLP transcriptional profiling reveals genes expressed during flower development in Oncidium Milliongolds

The DING family of proteins: ubiquitous in eukaryotes, but where are the genes?

The prohibitin genes in Arabidopsis thaliana: Expression in seeds, hormonal regulation and possible role in cell cycle control during seed germination

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