cDNA-AFLP transcriptional profiling reveals genes expressed during flower development in Oncidium Milliongolds

Abstract

ABSTRACT. The flower developmental process, which is crucial to the whole lifecycle of higher plants, is influenced by both environmental and endogenous factors. The genus Oncidium is commercially important for cut flower and houseplant industry and is ideal for flower development studies. Using cDNA-amplified restriction fragment length polymorphism analysis, we profiled transcripts that are differentially expressed during flower development of Oncidium Milliongolds. A total of 15,960 transcript-derived fragments were generated, with 114 primer sets. Of these, 1248 were sequenced, producing 993 readable sequences. BLASTX/N analysis showed that 833 of the 993 transcripts showed homology to genes in the NCBI databases, exhibiting functions involved in various processes, such as signal transduction, energy conversion, metabolism, and gene expression regulation. The full-length mRNAs of SUCROSE SYNTHASE 1 (SUS1) and LEAFY (LFY) were cloned, and their expression patterns were characterized. The results showed that the expression levels of SUS1 and LFY were similar during flower development. To confirm the function of SUS1 in flower buds, 6304 X. Qian et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6303-6315 (2014) carbohydrate content and sucrose synthase activity were determined. The results showed that changes in sucrose content and sucrose synthase activity reflected SUS1 expression levels. Collectively, these results indicate that SUS1 influences flower development by regulating LFY expression levels through changing the sucrose content of flower buds.

The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression

“…Large numbers of TDFs were differentially expressed in different developmental stages or diverse conditions, which were involved in various biological processes (Gupta et al, 2012 ). In this study, 328 TDFs were identified by differential expression in the early seedling stage under salt stress, and the GO enrichment and KEGG analysis showed that most of the TDFs were predominantly involved in transport, transcription, translation, carbohydrate transport, metabolism, protein synthesis, and protein fate ( Figure 12 ), which are consistent with those found in Oncidium milliongolds and Lavandula angustifolia (Qian et al, 2014 ; Banikamali et al, 2020 ). It has been reported that genes involved in metabolism and energy are activated early in seedlings and persist in functioning during growth (Guo et al, 2009 ).…”