The prohibitin genes in Arabidopsis thaliana: Expression in seeds, hormonal regulation and possible role in cell cycle control during seed germination

Diego, Juana Gutiérrez de; Rodríguez, Fernando Mateos; Lorenzo, José Luis Rodríguez; Cervantés, Emilio

doi:10.1016/j.jplph.2006.05.002

Cited by 8 publications

(6 citation statements)

References 14 publications

(14 reference statements)

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“…High AtPHB levels in proliferative tissues, such as the leaf primordia, the root apical meristem and lateral root initials, are in contrast to a previously suggested negative correlation of AtPHB4 mRNA levels and root growth rate (de Diego et al. , 2007).…”

Section: Discussioncontrasting

confidence: 67%

Mitochondrial type‐I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development

et al. 2007

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SummaryThe Arabidopsis thaliana genome expresses five evolutionarily conserved prohibitin (PHB) genes that are divided into type-I (AtPHB3 and AtPHB4) and type-II (AtPHB1, AtPHB2 and AtPHB6) classes, based on their phylogenetic relationships with yeast PHB1 and PHB2, respectively. Yeast and animal PHBs are reported to have diverse roles in the cell cycle, mitochondrial electron transport, aging and apoptosis. All transcribed Arabidopsis PHB genes are primarily expressed in both shoot and root proliferative tissues, where they are present in mitochondrial multimeric complexes. Loss of function of the type-I AtPHB4 had no phenotypic effects, while loss of function of the homologous AtPHB3 caused mitochondrial swelling, decreased meristematic cell production, increased cell division time and reduced cell expansion rates, leading to severe growth retardation. Double knockout atphb3 atphb4 plants were not viable, but transgenic lines overexpressing AtPHB3 or AtPHB4 showed leaf shape aberrations and an increased shoot branching phenotype. Genome-wide microarray analysis revealed that both knockout and overexpression perturbations of AtPHB3 and AtPHB4 provoked an altered abundance of mitochondrial and stress-related transcripts. We propose that plant type-I PHBs take part in protein complexes that are necessary for proficient mitochondrial function or biogenesis, thereby supporting cell division and differentiation in apical tissues.

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Section: Discussioncontrasting

confidence: 67%

Mitochondrial type‐I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development

et al. 2007

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“…Prohibitin participates in the regulation of E2F via interaction with E2Fs (Wang et al, 1999a(Wang et al, , 1999b. However, in plants, researchers have failed to detect the interaction between prohibitin and E2Fa or RBR1 using the yeast two-hybrid system, BiFC, Pulldown, and co-immunoprecipitation (De Diego et al, 2007;Supplemental Fig. S13), suggesting that the plant prohibitin protein may regulate cell proliferation via a different mechanism from mammals.…”

Section: Phb3 Regulates Cell Proliferation Through Regulating Mcm2 Exmentioning

confidence: 99%

Nuclear Prohibitin3 Maintains Genome Integrity and Cell Proliferation in the Root Meristem through Minichromosome Maintenance 2

et al. 2019

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The nucleo-mitochondrial dual-localized proteins can act as gene expression regulators; however, few instances of these proteins have been described in plants. Arabidopsis (Arabidopsis thaliana) PROHIBITIN 3 (PHB3) is involved in stress responses and developmental processes, but it is unknown how these roles are achieved at the molecular level in the nucleus. In this study, we show that nucleo-mitochondrial PHB3 plays an essential role in regulating genome stability and cell proliferation. PHB3 is upregulated by DNA damage agents, and the stress-induced PHB3 proteins accumulate in the nucleus. Loss of function of PHB3 results in DNA damage and defective maintenance of the root stem cell niche. Subsequently, the expression patterns and levels of the root stem cell regulators are altered and down-regulated, respectively. In addition, the phb3 mutant shows aberrant cell division and altered expression of cell cycle-related genes, such as CycB1 and Cyclin dependent kinase 1. Moreover, the minichromosome maintenance (MCM) genes, e.g. MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7, are up-regulated in the phb3 mutant. Reducing the MCM2 expression level substantially recovers the DNA damage in the phb3 mutant and partially rescues the altered cell proliferation and root deficiency of phb3 seedlings. PHB3 acts as a transcriptional coregulator that represses MCM2 expression by competitively binding to the promoter E2F-cis-acting elements with E2Fa so as to modulate primary root growth. Collectively, these findings indicate that nuclear-localized PHB3 acts as a transcriptional coregulator that suppresses MCM2 expression to sustain genome integrity and cell proliferation for stem cell niche maintenance in Arabidopsis.

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“…4,25 However, the same mechanism has not found in plants yet. 20,26 Arabidopsis PHB3 was mentioned to be interacted strongly in the yeast two-hybrid assay with a component of the TAFIID complex, Taf12b. 16 But the experiment data has not been shown yet.…”

Section: The Interacting Proteins Of the Phb3mentioning

confidence: 99%

“…19 Thus, PHB3 was thought to be one of the retrograde signaling resources from mitochondria to nucleus. 26 In spite of the PHB3 mutation can cause significant reactive oxygen species (ROS) accumulation, the retrograde signaling may tranduce into nucleus through the ROS-dependent and ROS-independent ways. 19,20,27 Phytohormone is very important for the plant development.…”

Section: The Function Of the Phb3mentioning

confidence: 99%

“…20 Moreover, the retrograde signalling in mutants defective in mitochondrial protein prohibitin phb3 can be suppressed by knocking out the transcription factor ANAC017. 26 Whether the PHB3 can bind on the promoter and regulate the expression of ANAC017 is deserved to be analyzed. Intriguingly, a putative PHB3-binding site with ATTCCCGG sequence, which conform to the Arabidopsis E2Fa specific binding site with a WTTSSCSS (where W = A or T and S = G or C) cis-acting consensus element, 32 was found in the 5ʹ UTR region of ANAC017.…”

Section: The Function Of the Phb3mentioning

confidence: 99%

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The Arabidopsis PHB3 is a pleiotropic regulator for plant development

Huang

Yang

Zhang

2019

Plant Signaling & Behavior

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Prohibitins (PHBs) are composed of an obviously conserved protein family in eukaryotic cells. Despite the extensive and in-depth research of mammalian PHB1 and PHB2, the plant prohibitins functions are not completely elucidated and little is known about their mechanism of action. This review focuses on the current knowledge about the protein subcellular localization, interaction proteins and target genes of PHB3. Furthermore, we discussed the roles of PHB3 protein in plant growth and development, plant responses to abiotic or biotic stresses and its participation in phytohormonal signaling.

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The prohibitin genes in Arabidopsis thaliana: Expression in seeds, hormonal regulation and possible role in cell cycle control during seed germination

Cited by 8 publications

References 14 publications

Mitochondrial type‐I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development

Mitochondrial type‐I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development

Nuclear Prohibitin3 Maintains Genome Integrity and Cell Proliferation in the Root Meristem through Minichromosome Maintenance 2

The Arabidopsis PHB3 is a pleiotropic regulator for plant development

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