“…This significant decrease was maintained for FcεRI + monocytes at T1 in both groups of patients and for mDCs at T1 in HCC patients. In addition, Meyerson et al reported that CD1c myeloid DCs (mDCs) were noted to have partial expression of CD14 (26). 2C) suggests that FcεRI + monocytes may represent those fated to become mDCs, what is further supported by our results on the phenotypic characterization of this cell population (Fig.…”
Section: Discussionsupporting
confidence: 86%
“…However, in differentiation experiments, it was reported that both culture-derived and primary CD1c + preconventional DCs purified from peripheral blood acquire CD14 expression in culture (25). In addition, Meyerson et al reported that CD1c myeloid DCs (mDCs) were noted to have partial expression of CD14 (26). Therefore, the classification of CD14 dim FcεRI + CD1c + cells into mDCs or monocytes subsets is controversial and still under debate.…”
Background: Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) represent the most common primary liver malignancies whose outcome is influenced by the immune response.Methods: In this study, we have functionally characterized, by flow cytometry, circulating myeloid dendritic cells (mDCs) and FcεRI + monocytes in a group of healthy individuals (n = 10) and in a group of patients with HCC (n = 19) and CCA (n = 8), at the time point of the surgical resection (T0) and once the patient had recovered from surgery (T1). Moreover, we proceeded to a more in depth phenotypic characterization of the FcεRI + monocyte subpopulation.Results: A significant decrease in the frequency of TNFα producing FcεRI + monocytes and mDCs in HCC and CCA patients when compared to the group of healthy individuals was observed, and a close association between FcεRI + monocytes and mDCs dysfunction was identified. In addition, the phenotypic characteristics of FcεRI + monocytes from healthy individuals strongly suggest that this population drives to mDCs, which matches with the fact that both populations are functionally affected.Conclusions: The frequency and the function of circulating mDCs and FcεRI + monocytes are affected in both HCC and CCA patients, and FcεRI + monocytes could represent those fated to become mDCs.
“…This significant decrease was maintained for FcεRI + monocytes at T1 in both groups of patients and for mDCs at T1 in HCC patients. In addition, Meyerson et al reported that CD1c myeloid DCs (mDCs) were noted to have partial expression of CD14 (26). 2C) suggests that FcεRI + monocytes may represent those fated to become mDCs, what is further supported by our results on the phenotypic characterization of this cell population (Fig.…”
Section: Discussionsupporting
confidence: 86%
“…However, in differentiation experiments, it was reported that both culture-derived and primary CD1c + preconventional DCs purified from peripheral blood acquire CD14 expression in culture (25). In addition, Meyerson et al reported that CD1c myeloid DCs (mDCs) were noted to have partial expression of CD14 (26). Therefore, the classification of CD14 dim FcεRI + CD1c + cells into mDCs or monocytes subsets is controversial and still under debate.…”
Background: Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) represent the most common primary liver malignancies whose outcome is influenced by the immune response.Methods: In this study, we have functionally characterized, by flow cytometry, circulating myeloid dendritic cells (mDCs) and FcεRI + monocytes in a group of healthy individuals (n = 10) and in a group of patients with HCC (n = 19) and CCA (n = 8), at the time point of the surgical resection (T0) and once the patient had recovered from surgery (T1). Moreover, we proceeded to a more in depth phenotypic characterization of the FcεRI + monocyte subpopulation.Results: A significant decrease in the frequency of TNFα producing FcεRI + monocytes and mDCs in HCC and CCA patients when compared to the group of healthy individuals was observed, and a close association between FcεRI + monocytes and mDCs dysfunction was identified. In addition, the phenotypic characteristics of FcεRI + monocytes from healthy individuals strongly suggest that this population drives to mDCs, which matches with the fact that both populations are functionally affected.Conclusions: The frequency and the function of circulating mDCs and FcεRI + monocytes are affected in both HCC and CCA patients, and FcεRI + monocytes could represent those fated to become mDCs.
“…Monocyte linage was previously published (19). The functional measurements of mDC's were also described (20). The authors report that the frequency and function of circulating mDC's and FceRI monocytes are reduced in both liver malignancies.…”
“…The paper by Depreter et al provides crucial information concerning the ability to detect single‐cell or rare cell population‐specific gene expression changes in acute myeloid leukemia (AML) samples. In both adult and pediatric AML, persistence of rare LSCs are thought to be causative for the high relapse rates, therefore, an increasing need for advanced instrument analytical tools, able to elucidate coding and non‐coding gene expressions at single‐cell level, has emerged, and this study may help in clarifying this aspect . …”
mentioning
confidence: 99%
“…Original articles published in this issue were related to four main fields immunophenotyping of lymphoproliferative disorders or normal cells, the detection of a rare acquired genetic disorder Paroxysmal nocturnal hemoglobinuria (PNH), immunology science with particular emphasis on monocytes, and cell function.…”
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