CD14 in the TLRs signaling pathway is associated with the resistance to E. coli F18 in Chinese domestic weaned piglets

Wu, Zhengchang; Liu, Ying; Dong, Wende; Zhu, Guoqiang; Wu, Shenglong; Bao, Wenbin

doi:10.1038/srep24611

Cited by 22 publications

(16 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, Meishan piglets were used as model animals to test their susceptibility to E. coli F18 by challenging with F18 strains. After a series of experiments, such as E. coli F18 bacteria detection, bacteria counting and adhesion test of the pathogens to the epithelial cells of small intestine in vitro, we strictly identified E. coli F18-resistant and -susceptible complete sib-pair individuals [18]. Solexa high-throughput sequencing technology is a convincing strategy for identifying miRNAs.…”

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs regulating Escherichia coli F18 infection in Meishan weaned piglets

Qin

Zhu

et al. 2016

Biol Direct

Self Cite

View full text Add to dashboard Cite

Background Escherichia coli F18 is mainly responsible for post-weaning diarrhea (PWD) in piglets. The molecular regulation of E. coli F18 resistance in Chinese domestic weaned piglets is still obscure. We used Meishan piglets as model animals to test their susceptibility to E. coli F18. Small RNA duodenal libraries were constructed for E. coli F18-sensitive and -resistant weaned piglets challenged with E. coli F18 and sequenced using Illumina Solexa high-throughput sequencing technology.ResultsSequencing results showed that 3,475,231 and 37,198,259 clean reads were obtained, with 311 known miRNAs differently expressed in resistant and sensitive groups, respectively. Twenty-four miRNAs, including 15 up-regulated and 9 down-regulated, demonstrated more than a 2-fold differential expression between the F18-resistant and -sensitive piglets. Stem-loop RT-qPCR showed that miR-136, miR-196b, miR-499-5p and miR-218-3p significantly expressed in intestinal tissue (p < 0.05). KEGG pathway analysis for target genes revealed that differently expressed miRNAs were involved in infectious diseases, signal transduction and immune system pathways. Interestingly, the expression of miR-218-3p in intestinal tissue had a very significant negative correlation with target DLG5 (P < 0.01).ConclusionsBased on the expression correlation between miRNA and target genes analysis, we speculate that miR-218-3p targeting to DLG5, appears to be very promising candidate for miRNAs involved in response to E. coli F18 infection. The present study provides improved database information on pig miRNAs, better understanding of the genetic basis of E. coli F18 resistance in local Chinese pig breeds and lays a new foundation for identifying novel markers of E. coli F18 resistance.ReviewersThis article was reviewed by Neil R Smalheiser and Weixiong Zhang.Electronic supplementary materialThe online version of this article (doi:10.1186/s13062-016-0160-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs regulating Escherichia coli F18 infection in Meishan weaned piglets

Qin

Zhu

et al. 2016

Biol Direct

Self Cite

View full text Add to dashboard Cite

show abstract

“…The second strand was synthesized based on the first strand with the base T replaced by U. To obtain more accurate results for the subsequent analysis in the gene functional annotation and expression, a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) that can determine the direction of justice chains and antisense chains was adopted to construct the libraries 71…”

Section: Methodsmentioning

confidence: 99%

Transcriptional profiling of Auricularia cornea in selenium accumulation

Yan

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Auricularia cornea is a widely cultivated edible fungus with substantial nutritive value. This study aimed to enrich the multifunctional bionutrient element selenium in A. cornea to improve its quality and explore the accumulation of selenium in the fungus using high-throughput RNA-Seq technology. In general, the treatment group with a 100 µg/g supply of selenium outperformed the other treatment groups in terms of high yield, rich crude polysaccharides and a high total selenium concentration. Additional evidences demonstrated the budding and mature phases were two typical growth stages of A. cornea and were important for the accumulation of selenium. Therefore, the budding and mature phase tissues of A. cornea in the treatment group with a 100 µg/g supply of selenium were used for transcriptome analysis and compared to those of a control group that lacked additional selenium. A total of 2.56 × 105 unigenes from A. cornea transcriptome were assembled and annotated to five frequently used databases including NR, GO, KEGG, eggNOG and SwissProt. GO and KEGG pathway analysis revealed that genes involved in metabolic process and translation were up-expressed at the budding stage in response to selenium supplementation, including amino acid metabolism, lipid metabolism, ribosome. In addition, the differential gene expression patterns of A. cornea suggested that the up-expressed genes were more likely to be detected at the budding stage than at the mature stage. These results provide insights into the transcriptional response of A. cornea to selenium accumulation.

show abstract

“…The products were purified (AMPure XP system) and quantified using the Agilent high-sensitivity DNA assay on the Bioanalyzer 2100 system (Agilent). The sequencing library was then sequenced on a HiSeq platform (Illumina) by Shanghai Personal Biotechnology Co., Ltd. [35,36].…”

Section: Transcriptome Analysis By Rna-sequencingmentioning

confidence: 99%

Staphylococcus aureus induces COX-2-dependent proliferation and malignant transformation in oral keratinocytes

Wang

Liu

et al. 2019

Journal of Oral Microbiology

View full text Add to dashboard Cite

The COX-2/PGE 2 axis can play roles in mediating the progression of tumor. COX-2 induction was observed in oral cancer. In our previous study, we found Staphylococcus aureus, a pathogen prevalent in oral cancer, can activate the COX-2/PGE 2 pathway in human oral keratinocyte (HOK) cells. Here, we investigated the proliferation of HOK cells affected by COX-2 induction and the role of COX-2 induction in the malignant transformation of HOK cells. We found S. aureus was able to facilitate HOK cell proliferation through upregulating COX-2 expression. With the induction of COX-2, expression of oral cancer-associated genes cyclin D1 was upregulated and p16 was downregulated. Transcriptome analysis showed that the "NF−kappa B signaling pathway" and "TNF signaling pathway" had the highest enrichment of differentially expressed genes (DEGs) with COX-2 over-expression. Seven upregulated genes (jun, tlr4, cxcl1, lif, cxcl3, tnfrsf1β, and il1β) in these two pathways were critical for the increased proliferation of HOK cells and might be associated with COX-2. Malignant transformation of cells was evaluated by soft agar colony formation assay and S. aureus infection promoted HOK cell colony formation. These results suggest the potential of S. aureus to induce the infection-associated malignant transformation of oral epitheliums through COX-2 activation.

show abstract

CD14 in the TLRs signaling pathway is associated with the resistance to E. coli F18 in Chinese domestic weaned piglets

Cited by 22 publications

References 32 publications

Identification of microRNAs regulating Escherichia coli F18 infection in Meishan weaned piglets

Identification of microRNAs regulating Escherichia coli F18 infection in Meishan weaned piglets

Transcriptional profiling of Auricularia cornea in selenium accumulation

Staphylococcus aureus induces COX-2-dependent proliferation and malignant transformation in oral keratinocytes

Contact Info

Product

Resources

About