Cavitation-generated free radicals during shock wave exposure: Investigations with cell-free solutions and suspended cells

Suhr, Dierk; Brümmer, Franz; Hülser, Dieter F.

doi:10.1016/0301-5629(91)90158-s

Cited by 90 publications

(43 citation statements)

References 33 publications

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“…Along with the mechanical effects of cavitation -surface ablation by jet streams of high velocity accelerated from asymmetrically collapsing bubbles there also exist chemical effects mediated by cavitation-generated free radicals. With different probes sensitive to oxidation, free radicals have been demonstrated after shock-wave application to cell free solutions 7,8,19.…”

Section: Resultsmentioning

confidence: 99%

Reduced cavitation-induced cellular damage by the antioxidative effect of vitamin E

et al. 1994

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Fragmentation of human urinary and biliary stones by shock waves in extracorporeal lithotripsy is accompanied by tissue damage. Both the fragmentation as well as the side effects are often attributed to cavitation. The hazardous potential of cavitation is not only of a physical nature but also of a chemical nature, because of the generation of free radicals, e.g..OH, .H and '02. After the application of shock waves, we have demonstrated cavitation-generated free radicals in cell-free solutions and also in the surviving and intact suspended MGH-U1 cells by hydroethidine measurements. Under electron microscopical inspection, the same cells exhibited perinuclear cisternae, damaged mitochondria and numerous intracellular vacuoles. The contribution of free radicals to cell damage was investigated by reducing the vitamin E level in rats by a tocopherol free diet and by incubating L1 210 cells in a tocopherol enriched medium. After 250 shock waves, ex vivo erythrocytes revealed a 75% increase in total cell disruption over cells from non-depleted rats. The in vitro experiments with L1 210 cells exhibited a moderate protection by the addition of this scavenger of free radicals. Keywords: lithotripsy; shock waves; free radicals; tocopherol; in vitro; ex vivoSince 1980 a new technology for the treatment of urinary and biliary calculi has revolutionized the treatment of stone disease. Shock waves -generated extracorporeally by underwater spark discharge, electromagnetic emitters or piezoceramic paraboloids -disintegrate stones inside the human body. The so-called extracorporeal shock wave lithotripsy is a very successful technique, which is applied to most kidney stone patients. The shock-wave treatment, however, is accompanied by side effects like pain, skin petechiae or ecchimosis, haematuria, and organ injury (for reviews see References 1 3).Cell cultures are damaged or destroyed in a dose dependent manner when cells are treated in suspension 4. This damage is not related to the origin of cells and is similar for normal and malignant cells 5, therefore, a physical or chemical cause for these cellular injuries must be assumed which overrules possible biological differences. One major mechanism responsible for these side effects is cavitation, i.e. the generation and movement of bubbles in liquids exposed to tensile stresses. During the collapse of these cavitation bubbles, gas or vapour inside the bubble is nearly adiabatically compressed without heat exchange with the surrounding medium 6. This results in local 'hot spots', where the high temperature can lead to a homolytic cleavage of molecules, thus generating free Paper presented at the "Free Radicals and Ultrasound in Chemistry and Medicine" conference held at the Scientific Societies Lecture Theatre, London, 17-18 December 1992 radicals. These cavitation-induced short lived and highly reactive atoms or molecules have been detected in water by the oxidation of iodide to iodine or Fe(II) to Fe(III) after treatment with shock waves 7,a. Their contribution to cell killi...

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Section: Resultsmentioning

confidence: 99%

Reduced cavitation-induced cellular damage by the antioxidative effect of vitamin E

et al. 1994

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“…2 Concerning the mechanism of the adverse effects of ESW, although initially they were attributed to renal damage resulting only from the direct action of cavitation bubbles or shear stress originating from shock-wave energy, more recently free oxygen and nitrogen radical production originating from transient ischemia due to ESW application was considered to be an integral element in shock wave-induced renal damage through an indirect mechanism. 5,6 Transient ischemia and following reperfusion causes release of cytokines/inflammatory cellular mediators, production of oxygenderived (reactive oxygen species ROS) free radicals, such as superoxide (O 2 ), peroxide (H 2 O 2 ) and hydroxyl radicals (OH À ), production of nitrogen-derived free radicals (reactive nitrogen species RNS), such as nitric oxide (NO) peroxynitrite (ONOO À ), and infiltration of tissue by inflammatory response cells and this process initiates the cellular damage and death. Which treatment with free radical scavengers has been found to be effective in terms of survival and renal function in experimental and clinical studies supports this hypothesis.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of poly(adenosine diphosphate-ribose) polymerase inhibition in extracorporeal shock wave-induced renal injury

Malkoç

Fırat²,

Demirer

et al. 2014

Renal Failure

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Objectives: Extracorporeal shock wave lithotripsy (ESW) induces renal damage by excessive production of free oxygen radicals. Free Oxygen radicals cause cellular injury by inducing nicks in DNA. The enzyme poly(adenosine diphosphate-ribose) polymerase (PARP) involved in the process of repair of DNA in damaged cells. However, its activation in damaged cells can lead to adenosine triphosphate depletion and death. Thus, we designed a study to evaluate the efficacy of 3-aminobenzamide (3-AB), a PARP inhibitor, against extracorporeal shock wave induced renal injury. Methods: Twenty-four Sprague-Dawley rats were divided into three groups: control, ESW, ESW + 3-AB groups. All groups except control group were subjected to ESW procedure. ESW + 3-AB group received 20 mg/kg/day 3-aminobenzamide intraperitoneally at 2 h before ESW and continued once a day for consecutive 3 days. The surviving animals were sacrificed at the 4th day and their kidneys were harvested for biochemical and histopathologic analysis. Blood samples from animals were also obtained. Results: Serum ALT and AST levels, serum neopterin and tissue oxidative stress parameters were increased in the ESW group and almost came to control values in the treatment group (p50.05, ESW vs. ESW + 3-AB). Histopathological injury score were significantly lower in treatment group than the ESW group (p50.05, ESW vs. ESW + 3-AB). Conclusion: Our data showed that PARP inhibition protected renal tissue against ESW induced renal injury. These findings suggest that it would be possible to improve the outcome of ESW induced renal injury by using PARP inhibitors as a preventive therapy.

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“…Therefore we performed a cytofluorimetric analysis of SW-treatment induced cell death after 24 h, in order to investigate whether cancer cell growth reduction was due to apoptotic and/or necrotic cell death. Our results highlighted very significant increases in both apoptotic and necrotic cells in SW-treated MSC/cancer cell co-cultures, as compared to untreated co-cultures, as 25 the cell death of MSC and cancer cells was due to the ROS burst selectively induced in MSC by SW exposure.…”

Section: Discussionmentioning

confidence: 58%

“…According to this model, ultrasound with specific characteristics can induce modifications in the lipid bilayer of cell membrane leading to intracellular deformations. Moreover, under appropriate conditions, a significant oxidative stress may occur during SW exposure due to the imbalance between ROS production and antioxidant defence [24], resulting in ROS-induced cytotoxicity [25]. However, the in vivo treatment of solid tumors by SW alone has been shown to be ineffective [26].…”

Section: Introductionmentioning

confidence: 99%

Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

et al. 2017

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Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell cocultures / Foglietta, Federica; Duchi, Serena; Canaparo, Roberto; Varchi, Greta; Lucarelli, Enrico; Dozza, Barbara; Serpe, Loredana. -In: LIFE SCIENCES. -ISSN 0024-3205. -173(2017), pp. 28-35. Original Citation:Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy. AbstractAim: Mesenchymal stem cells (MSC) possess the distinctive feature of homing in on and engrafting into the tumor stroma making their therapeutic applications in cancer treatment very promising.Research into new effectors and external stimuli, which can selectively trigger the release of cytotoxic species from MSC toward the cancer cells, significantly raises their potential.Main methods: Shock waves (SW) have recently gained recognition for their ability to induce specific biological effects, such as the local generation of cytotoxic reactive oxygen species (ROS) in a non-invasive and tunable manner. We thus investigate whether MSC are able to generate ROS and, in turn, affect cancer cell growth when in co-colture with human glioblastoma (U87) or osteorsarcoma (U2OS) cells and exposed to SW.Key findings: MSC were found to be the cell line that was most sensitive to SW treatment as shown by SW-induced ROS production and cytotoxicity. Notably, U87 and U2OS cancer cell growth was unaffected by SW exposure. However, significant decreases in cancer cell growth, 1.8 fold for U87 and 2.3 fold for U2OS, were observed 24 h after the SW treatment of MSC co-cultures with cancer cells. The ROS production induced in MSC by SW exposure was then responsible for lipid peroxidation and cell death in U87 and U2OS cells co-cultured with MSC.Significance: This experiment highlights the unique ability of MSC to generate ROS upon SW treatment and induce the cell death of co-cultured cancer cells. SW might therefore be proposed as an innovative tool for MSC-mediated cancer treatment.3

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Cavitation-generated free radicals during shock wave exposure: Investigations with cell-free solutions and suspended cells

Cited by 90 publications

References 33 publications

Reduced cavitation-induced cellular damage by the antioxidative effect of vitamin E

Reduced cavitation-induced cellular damage by the antioxidative effect of vitamin E

Efficacy of poly(adenosine diphosphate-ribose) polymerase inhibition in extracorporeal shock wave-induced renal injury

Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

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