Reduced cavitation-induced cellular damage by the antioxidative effect of vitamin E

Suhr, Dierk; Brümmer, Franz; Irmer, Ulrich; Schlachter, M.; Hülser, Dieter F.

doi:10.1016/0041-624x(94)90010-8

Cited by 40 publications

(14 citation statements)

References 36 publications

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“…Conversely, the preincubation of MSC with NAC induced a significantly lower decrease in co-culture cell growth, thus confirming the ROS-mediated action of the MSC/SW system [46,47]. Our results are in agreement with a previously reported study, which describes the generation of ROS during cavitation and how the presence of a free radical-scavenger diminishes cells damage [40].…”

Section: Discussionsupporting

confidence: 83%

“…It is generally accepted that one of the main effectors of the non-thermal interactions between ultrasound and biological tissues is acoustic cavitation and that it is able to generate short-lived species, such as ROS and free radicals, thanks to its high-energy release [39,40]. It has been reported that ultrasound alone, which results in a cavitation process, can induce time-dependent apoptotic cell death in in vitro liquid tumors, such as human myeloid leukemia and histiocytic lymphoma [41,42].…”

Section: Discussionmentioning

confidence: 99%

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Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

et al. 2017

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Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell cocultures / Foglietta, Federica; Duchi, Serena; Canaparo, Roberto; Varchi, Greta; Lucarelli, Enrico; Dozza, Barbara; Serpe, Loredana. -In: LIFE SCIENCES. -ISSN 0024-3205. -173(2017), pp. 28-35. Original Citation:Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy. AbstractAim: Mesenchymal stem cells (MSC) possess the distinctive feature of homing in on and engrafting into the tumor stroma making their therapeutic applications in cancer treatment very promising.Research into new effectors and external stimuli, which can selectively trigger the release of cytotoxic species from MSC toward the cancer cells, significantly raises their potential.Main methods: Shock waves (SW) have recently gained recognition for their ability to induce specific biological effects, such as the local generation of cytotoxic reactive oxygen species (ROS) in a non-invasive and tunable manner. We thus investigate whether MSC are able to generate ROS and, in turn, affect cancer cell growth when in co-colture with human glioblastoma (U87) or osteorsarcoma (U2OS) cells and exposed to SW.Key findings: MSC were found to be the cell line that was most sensitive to SW treatment as shown by SW-induced ROS production and cytotoxicity. Notably, U87 and U2OS cancer cell growth was unaffected by SW exposure. However, significant decreases in cancer cell growth, 1.8 fold for U87 and 2.3 fold for U2OS, were observed 24 h after the SW treatment of MSC co-cultures with cancer cells. The ROS production induced in MSC by SW exposure was then responsible for lipid peroxidation and cell death in U87 and U2OS cells co-cultured with MSC.Significance: This experiment highlights the unique ability of MSC to generate ROS upon SW treatment and induce the cell death of co-cultured cancer cells. SW might therefore be proposed as an innovative tool for MSC-mediated cancer treatment.3

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

et al. 2017

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“…An increase in HE-derived fluorescence intensity was observed after a shock wave treatment of cell suspension or a cell-free system containing HE [104,105]. It was shown that the increase in HE-derived fluorescence after treating cells with shock waves correlates directly with the cell membrane damage and increased membrane permeability, thus confounding the interpretation of the results of the flow cytometry measurements [106].…”

Section: Reaction Between He and Oxidantsmentioning

confidence: 99%

Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: Another inconvenient truth

Zielonka

Kalyanaraman

2010

Free Radical Biology and Medicine

450

399

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Hydroethidine (or dihydroethidium) (HE) is the most popular fluorogenic probe used for detecting intracellular superoxide radical anion. The reaction between superoxide and HE generates a highly specific red fluorescent product, 2-hydroxyethidium (2-OH-E+). In biological systems, another red fluorescent product, ethidium (E+), is also formed, usually at a much higher concentration than 2-OH-E+. In this article, we have reviewed the methods to selectively detect the superoxide-specific product (2-OH-E+) and the factors affecting its levels in cellular and biological systems. The most important conclusion of the present review is that it is nearly impossible to assess the intracellular levels of the superoxide specific product, 2-OH-E+, using the confocal microscopy or other fluorescence-based microscopic assays and that it is essential to measure by HPLC the intracellular HE and other oxidation products of HE, in addition to 2-OH-E+, in order to fully understand the origin of red fluorescence. The chemical reactivity of mitochondria-targeted hydroethidine (Mito-HE, MitoSOX Red ®) with superoxide is similar to the reactivity of HE with superoxide and therefore, all of the limitations attributed to the HE assay are applicable to Mito-HE (or Mito-SOX) as well.

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“…Because shock waves have been shown to produce free radicals in isolated cells [21, 22, 23], it is possible that the elevated basal [Ca 2+ ] i in SWE-treated cells was due to increased cell permeability induced by free radicals. The fact that allopurinol, which reduces free radical formation by inhibiting xanthine oxidase [24], blocked rises in basal [Ca 2+ ] i in SWE-treated MDCK cells [Jan, unpubl.…”

Section: Discussionmentioning

confidence: 99%

High-Energy Shock Waves Alter Cytosolic Calcium Mobilization in Single MDCK Cells

Jan¹,

Huang²,

Tseng³

1998

Nephron

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The damaging effect of shock waves on intracellular calcium homeostasis in renal tubular cells has not been investigated previously. We have examined the effects of shock waves on Madin Darby canine kidney (MDCK) cells by determining the release of two cellular enzymes: glutamate oxalactate transferase (GOT) and lactate dehydrogenase (LDH); and both the basal cytosolic calcium concentration ([Ca²⁺]_i) and the [Ca²⁺]_i rises evoked by Ca²⁺-mobilizing agonists after shock wave exposure (SWE). Immediately but transiently after SWE, the release of GOT and LDH increased by 15% and 5-fold, respectively, implicating cell membrane damage; and within 1–7 h after SWE, basal [Ca²⁺]_i was elevated by 15–141%. In SWE-treated cells, the peak amplitude of the [Ca²⁺]_i transients evoked by ATP, bradykinin, thapsigargin and 2,5-Di-(t-butyl)-1,4-hydroquinone (BHQ) was slightly altered, and the plateau amplitude was markedly elevated. The ATP and bradykinin receptors, the Ca²⁺ pump on the endoplasmic reticulum membrane, and the effector molecules involved in regulating the [Ca²⁺]_i transients were slightly affected. Shock waves appeared to cause a mild impairment in the mechanisms responsible for the decay of the evoked [Ca²⁺]_i rises.

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Reduced cavitation-induced cellular damage by the antioxidative effect of vitamin E

Cited by 40 publications

References 36 publications

Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: Another inconvenient truth

High-Energy Shock Waves Alter Cytosolic Calcium Mobilization in Single MDCK Cells

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