Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures

Abstract

Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell cocultures / Foglietta, Federica; Duchi, Serena; Canaparo, Roberto; Varchi, Greta; Lucarelli, Enrico; Dozza, Barbara; Serpe, Loredana. -In: LIFE SCIENCES. -ISSN 0024-3205. -173(2017), pp. 28-35. Original Citation:Selective sensitiveness of mesenchymal stem cells to shock waves leads to anticancer effect in human cancer cell co-cultures You may download, copy and otherwise use the AAM for non-commercial purposes provided that your license is limited by the following restrictions:(1) You may use this AAM for non-commercial purposes only under the terms of the CC-BY-NC-ND license.(2) The integrity of the work and identification of the author, copyright owner, and publisher must be preserved in any copy. AbstractAim: Mesenchymal stem cells (MSC) possess the distinctive feature of homing in on and engrafting into the tumor stroma making their therapeutic applications in cancer treatment very promising.Research into new effectors and external stimuli, which can selectively trigger the release of cytotoxic species from MSC toward the cancer cells, significantly raises their potential.Main methods: Shock waves (SW) have recently gained recognition for their ability to induce specific biological effects, such as the local generation of cytotoxic reactive oxygen species (ROS) in a non-invasive and tunable manner. We thus investigate whether MSC are able to generate ROS and, in turn, affect cancer cell growth when in co-colture with human glioblastoma (U87) or osteorsarcoma (U2OS) cells and exposed to SW.Key findings: MSC were found to be the cell line that was most sensitive to SW treatment as shown by SW-induced ROS production and cytotoxicity. Notably, U87 and U2OS cancer cell growth was unaffected by SW exposure. However, significant decreases in cancer cell growth, 1.8 fold for U87 and 2.3 fold for U2OS, were observed 24 h after the SW treatment of MSC co-cultures with cancer cells. The ROS production induced in MSC by SW exposure was then responsible for lipid peroxidation and cell death in U87 and U2OS cells co-cultured with MSC.Significance: This experiment highlights the unique ability of MSC to generate ROS upon SW treatment and induce the cell death of co-cultured cancer cells. SW might therefore be proposed as an innovative tool for MSC-mediated cancer treatment.3

Zinc Oxide Nanocrystals and High-Energy Shock Waves: A New Synergy for the Treatment of Cancer Cells

“…The analyses started looking for the safest conditions for KB cells under SW stimulation in absence of ZnO NCs. Based on the previous literature investigations (Canaparo et al, 2006(Canaparo et al, , 2013Serpe et al, 2011;Foglietta et al, 2017), a fixed number of shots (500 or 1000) was adopted, varying the PPP (29.1, 39.4, 50.3, 61.7, and 74.1 MPa). The cell viability decreased at increasing either the SW energies (from 29.1 MPa up to 74.1 MPa) or the number of shots (either 500 or 1000 shots), as evidenced in Figure 4.…”

“…KB cells, seeded into a treated culture flask, in exponential growth phase, where trypsinized and 5 × 10 5 cells per well were plated in culture medium into a 96-well plate (Corning) for the SW treatment as described by others (Canaparo et al, 2008(Canaparo et al, , 2013Foglietta et al, 2017).…”

Remotely Activated Nanoparticles for Anticancer Therapy

“…SW have also been proposed alone to inhibit the growth of cancer cells. Foglietta et al reported that when mesenchymal stem cells and cancer cells (glioblastoma or osteosarcoma) co-cultures are irradiated with SW, the consequence is a selective cancer cell death related to the ROS production by mesenchymal stem cells [ 163 ]. Otherwise, SW could be employed to permeabilize temporarily cell membrane and improve the uptake of drugs and chemotherapies [ 164 , 165 ], as well for the delivery of nucleic acids [ 166 , 167 ].…”