Causes, Diagnostics, and Distribution of an Ongoing Penaeid Shrimp Black Gill Epidemic in the U.S. South Atlantic Bight

Frischer, Marc E.; Lee, Richard F.; Price, Ashleigh; Walters, Tina L.; Bassette, Molly; Verdiyev, Rufat; Torris, Michael C.; Bulski, Karrie; Geer, Patrick J.; Powell, Shirley; Walker, Anna N.; Landers, Stephen C.

doi:10.2983/035.036.0220

Cited by 24 publications

(40 citation statements)

References 43 publications

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“…In silico testing utilizing the Silva TestPrime utility (Quast et al, ) indicated that this primer set matches with full identity (0 mismatches) with 10.8% of all diatom species included in the Silva reference database v132. In practice, however, 18S rDNA‐targeted primers are generally effective in amplifying targets containing up to three mismatches depending on assay conditions (Frischer et al, ). The primer set developed here would be expected to amplify 17.9%, 34.3% and 61.1% of all diatom species in the Silva reference database (r132) allowing for one, two and three mismatches, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Sequencing of barcoded amplicon libraries was accomplished using Ion Torrent procedures on a Personal Genome Machine (PGM) as previously described by Frischer et al (). Briefly, barcoded libraries from pooled samples prepared from doliolids and water samples collected from each cruise were prepared from randomly sheared (~400 bp) preparations of the 630 bp 18S rRNA amplicon and were sequenced on a 316v2 chip with 400 bp chemistry.…”

Section: Methodsmentioning

confidence: 99%

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Diet and trophic interactions of a circumglobally significant gelatinous marine zooplankter,Dolioletta gegenbauri(Uljanin, 1884)

et al. 2018

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Gelatinous zooplankton play a crucial role in marine planktonic food webs. However, primarily due to methodological challenges, the in situ diet of zooplankton remains poorly investigated and little is known about their trophic interactions including feeding behaviour, prey selection and in situ feeding rates. This is particularly true for gelatinous zooplankton including the marine pelagic tunicate, Dolioletta gegenbauri. In this study, we applied an 18S rRNA amplicon metabarcoding approach to identify the diet of captive‐fed and wild‐caught D. gegenbauri on the midcontinental shelf of the South Atlantic Bight, USA. Sequencing‐based approaches were complimented with targeted quantitative real‐time polymerase chain reaction (PCR) analyses. Captive‐fed D. gegenbauri gut content was dominated by pico‐, nano‐ and micro‐plankton including pico‐dinoflagellates (picozoa) and diatoms. These results suggested that diatoms were concentrated by D. gegenbauri relative to their concentration in the water column. Analysis of wild‐caught doliolids by quantitative real‐time PCR utilizing a group‐specific diatom primer set confirmed that diatoms were concentrated by D. gegenbauri, particularly by the gonozooid life stage associated with actively developing blooms. Sequences derived from larger metazoans were frequently observed in wild‐caught animals but not in captive‐fed animals suggesting experimental bias associated with captive feeding. These studies revealed that the diet of D. gegenbauri is considerably more diverse than previously described, that parasites are common in wild populations, and that prey quality, quantity and parasites are likely all important factors in regulating doliolid population dynamics in continental shelf environments.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Diet and trophic interactions of a circumglobally significant gelatinous marine zooplankter,Dolioletta gegenbauri(Uljanin, 1884)

et al. 2018

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“…Sequencing of barcoded amplicon libraries was accomplished using Ion Torrent procedures on a Personal Genome Machine (PGM) as previously described by (Frischer et al 2017). Barcoded libraries from pooled samples prepared from doliolids and water samples collected from each cruise were prepared from randomly sheared (ca.…”

Section: Gut Content Assessment By Metabarcoding Next Generation Sequencingmentioning

confidence: 99%

Selective feeding and linkages to the microbial food web by the doliolidDolioletta gegenbauri

Frischer

Lamboley

Walters

et al. 2021

Limnology & Oceanography

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Gelatinous zooplankton play a crucial role in pelagic marine food webs, however, due to methodological challenges and persistent misconceptions of their importance, the trophic role of gelatinous zooplankton remains poorly investigated. This is particularly true for small gelatinous zooplankton including the marine pelagic tunicate, Dolioletta gegenbauri. D. gegenbauri and other doliolid species occur persistently on wide subtropical shelves where they often produce massive blooms in association with shelf upwelling conditions. As efficient filter feeders and prodigious producers of relatively low-density organic-rich aggregates, doliolids are understood to contribute significantly to shelf production, pelagic ecology, and pelagic-benthic coupling. Utilizing molecular gut content analysis and stable isotope analysis approaches, the trophic interactions of doliolids were explored during bloom and nonbloom conditions on the South Atlantic Bight continental shelf in the Western North Atlantic. Based on molecular gut content analysis, relative ingestion selectivity varied with D. gegenbauri life stage. At all life stages, doliolids ingested a wide range of prey types and sizes, but exhibited selectivity for larger prey types including diatoms, ciliates, and metazoans. Experimental growth studies confirmed that metazoan prey were ingested, but indicated that they were not digested and assimilated. Stable isotopic composition (δ 13 C and δ 15 N) of wild-caught doliolids, during bloom and non-bloom conditions, were most consistent with a detrital-supplemented diet. These observations suggest that the feeding ecology of D. gegenbauri is more complex than previously reported, and have strong and unusual linkages to the microbial food web.

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“…As oxygen concentrations drop with increasing temperature, the physical impairment intensifies stress on the host. Because temperature stress seemingly accentuates disease pathology, it is not surprising that the highest prevalence of the late-stage symptomatic expression of the disease is found in late summer as water temperatures peak [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of climate change on parasites and disease in estuarine and nearshore environments

Byers

2020

PLoS Biol

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Information on parasites and disease in marine ecosystems lags behind terrestrial systems, increasing the challenge of predicting responses of marine host–parasite systems to climate change. However, here I examine several generalizable aspects and research priorities. First, I advocate that quantification and comparison of host and parasite thermal performance curves is a smart approach to improve predictions of temperature effects on disease. Marine invertebrate species are ectothermic and should be highly conducive to this approach given their generally short generation times. Second, in marine systems, shallow subtidal and intertidal areas will experience the biggest temperature swings and thus likely see the most changes to host–parasite dynamics. Third, for some responses like parasite intensity, as long as the lethal limit of the parasite is not crossed, on average, there may be a biological basis to expect temperature-dependent intensification of impacts on hosts. Fourth, because secondary mortality effects and indirect effects of parasites can be very important, we need to study temperature effects on host–parasite dynamics in a community context to truly know their bottom line effects. This includes examining climate-influenced effects of parasites on ecosystem engineers given their pivotal role in communities. Finally, other global change factors, especially hypoxia, salinity, and ocean acidity, covary with temperature change and need to be considered and evaluated when possible for their contributing effects on host–parasite systems. Climate change–disease interactions in nearshore marine environments are complex; however, generalities are possible and continued research, especially in the areas outlined here, will improve our understanding.

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Causes, Diagnostics, and Distribution of an Ongoing Penaeid Shrimp Black Gill Epidemic in the U.S. South Atlantic Bight

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 24 publications

References 43 publications

Diet and trophic interactions of a circumglobally significant gelatinous marine zooplankter,Dolioletta gegenbauri(Uljanin, 1884)

Diet and trophic interactions of a circumglobally significant gelatinous marine zooplankter,Dolioletta gegenbauri(Uljanin, 1884)

Selective feeding and linkages to the microbial food web by the doliolidDolioletta gegenbauri

Effects of climate change on parasites and disease in estuarine and nearshore environments

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