Cationic Magnetite Nanoparticles for Increasing siRNA Hybridization Rates

Abstract

An investigation of the interaction principles of nucleic acids and nanoparticles is a priority for the development of theoretical and methodological approaches to creating bionanocomposite structures, which determines the area and boundaries of biomedical use of developed nanoscale devices. «Nucleic acid—magnetic nanoparticle» type constructs are being developed to carry out the highly efficient detection of pathogens, create express systems for genotyping and sequencing, and detect siRNA. However, the data available on the impact of nanoparticles on the behavior of siRNA are insufficient. In this work, using nanoparticles of two classical oxides of inorganic chemistry (magnetite (Fe3O4) and silica (SiO2) nanoparticles), and widely used gold nanoparticles, we show their effect on the rate of siRNA hybridization. It has been determined that magnetite nanoparticles with a positive charge on the surface increase the rate of siRNA hybridization, while negatively charged magnetite and silica nanoparticles, or positively charged gold nanoparticles, do not affect hybridization rates (HR).

The Effect of pH and Buffer on Oligonucleotide Affinity for Iron Oxide Nanoparticles

“…Magnetofection is an approach combining magnetic targeting an external field and nucleic acid delivery [28][29][30][31][32]. The magnetofection using MNPs encompass various nucleic acid types, such as DNA [33][34][35], small interfering RNA (siRNA) [35][36][37][38][39][40], micro RNA (miRNA) [41], and aptamers [42][43][44][45], have shown great potential in nanomedicine and fundamental investigations. However, the constructions conducted with nucleic acids represent a hard-obtained feature of multilayer nanocontainers.…”

Nanomaterial Shape Influence on Cell Behavior

“…It is necessary to distinguish clearly the effects of particle shape from the functionalization of the surface, charge, size, and other properties. Occasionally, non-obvious properties such as charge density [ 202 ], crystallinity [ 203 ], aggregation state [ 99 ], and the structure of local inhomogeneities [ 204 ], are essential. Thus, it is necessary to optimize the synthesis procedures and create particles which differ only in their shape.…”