Catecholamines increase nerve growth factor mRNA content in both mouse astroglial cells and fibroblast cells

Abstract

Previous studies have shown that catecholamines increase the nerve growth factor (NGF) content in medium conditioned by mouse L-M fibroblast cells and mouse astroglial cells. In this study, the NGF mRNA levels in these cells were measured by Northern blot analysis. In astroglial cells treated with epinephrine (EN), the cellular NGF mRNA level increased prior to accumulation of NGF in the culture medium. 3-Hydroxytyramine (DA) and norepinephrine (NE) also increased the cellular NGF mRNA content. An increased level of NGF mRNA elicited by EN was also observed in mouse L-M oells. These results indicate that catecholamines enhance NGF synthesis of L-M fibroblast cells and astroglial cells by in~easing the cellular content of NGF mRNA. The present results also indicate that the effects of cat~hola~nes are not mediated by adrenergic receptors.

Effects of Nerve Growth Factor and Dimethylsulfoniopropionate in Green Sea Algae on the Outgrowth of Neurites from Pheochromocytoma Cells

“…Moreover, there is a noticeworthy report that catecholamines increase the amounts of NGF in the astroglial and fibroblast cells originating from mice (40). Therefore, the obtained results may provide evidence that DMSP increases the amounts of catecholamines (17,19,22), and thus elevates the NGF amounts (40), which promotes the outgrowth and elongation of neurites from the nerve cells.…”

“…Moreover, there is a noticeworthy report that catecholamines increase the amounts of NGF in the astroglial and fibroblast cells originating from mice (40). Therefore, the obtained results may provide evidence that DMSP increases the amounts of catecholamines (17,19,22), and thus elevates the NGF amounts (40), which promotes the outgrowth and elongation of neurites from the nerve cells. These findings may suggest that DMSP, which directly incorporates and accumulates catecholamines into the livers and brains in the experiments with rats and mice (17,19,22), more effectively not only prevents and/or ameliorates the neuronal development from the retardation and denaturation of the central nervous system in a diseased brain (Alzheimer and Parkinson diseases) and the aging (especially dimentia) of humans, but also from various injuries of the peripheral and central nervous system in humans (28,41), accompanied by the increase of NGF.…”

Tobacco Tar Components That Stimulate Nerve Growth Factor (NGF) Synthesis/Secretion of Mouse Astroglial Cells in Culture

“…Because the induction of NGF synthesis/secretion of NGF in astroglial cells by catechol derivatives occured with the increment of NGF mRNA content, these test samples also may have the same pathway. 12) To analyze the NGF synthesized by nicotine-treated astroglial cells, we measured by gel filtration the molecular size of the NGF produced. Figure 6A shows the molecular sizes of NGF synthesized/secreted by astroglial cells stimulated with I-nicotine.…”

Significant Effect of Dimethylsulfoniopropionate on Parkinson's Disease of Senescence-Accelerated Mice Induced by 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine

“…In contrast, there are significant reports that catecholamines promote the synthesis of nerve growth factor (NGF) in the astroglial and fibroblast cells in the mouse central nervous system ( 18 ), that NGF releases the amyloid precursor from the nerve model cells, PC12 cells ( 19 ), and that DMSP stimulates the outgrowth and elongation of neurites from the PC12 cells ( 20 ) and significantly recovers them from the cells inhibited by MPTP ( 21 ).…”