Catalytic activity of bovine lactoperoxidase supported on macroporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)

Nino, Giovanna Di; Turacchio, Manola; D’Archivio, Angelo Antonio; Lora, S.; Corain, Benedetto; Antonini, Giovanni

doi:10.1016/j.reactfunctpolym.2004.08.005

Cited by 8 publications

(1 citation statement)

References 29 publications

(34 reference statements)

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“…Several other publications deal with copolymers of GMA and 2-hydroxyethyl methacrylate and their use for the immobilization of enzymes − . In contrast, little research has so far been done in the field of immobilization of antibodies using GMA.…”

Section: Introductionmentioning

confidence: 99%

Surfactant-Free Poly(styrene-co-glycidyl methacrylate) Particles with Surface-Bound Antibodies for Activation and Proliferation of Human T Cells

et al. 2010

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In this article, we present our results on the design of new polymeric carriers for antibodies. Polymer colloids based on poly(styrene-co-glycidyl methacrylate) were synthesized by surfactant-free emulsion polymerization. Obtained polymer particles stabilized by grafted poly(ethylene glycol) (PEG) chains and carrying active epoxy groups were used for the covalent immobilization of activating antibodies against the human surface proteins CD (cluster of differentiation) 3 and CD28. The particle-antibody conjugates were employed for the stimulation of human CD4 memory T cells. This was analyzed by the up-regulation of the activation markers CD69 and CD25 on T cells and T cell proliferation as assessed by the dilution of a fluorescent dye on dividing daughter T cells. The particle-antibody conjugates were able to stimulate T cells at least as efficiently as conventional methods, e.g., surface-immobilized antibodies. Furthermore, an increase of the PEG chain length of the particles decreased the efficiency of the particle-antibody conjugates to activate T cells.

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Section: Introductionmentioning

confidence: 99%

Surfactant-Free Poly(styrene-co-glycidyl methacrylate) Particles with Surface-Bound Antibodies for Activation and Proliferation of Human T Cells

et al. 2010

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Immobilization of Enzymes on 2‐Hydroxyethyl Methacrylate and Glycidyl Methacrylate Copolymer Brushes

Ren

Mao

Moya

et al. 2014

Chemistry An Asian Journal

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The immobilization of enzymes is of paramount importance to maintain their activity and stability. In this study, surface-initiated atom-transfer radical polymerization was applied to prepare poly(2-hydroxyethyl methacrylate)-block-poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) brushes on glass slides. The polymerization kinetics was followed by using a quartz crystal microbalance with dissipation monitoring and ellipsometry in terms of mass and thickness growth, respectively. The surface chemical compositions of the obtained polymer brushes were characterized by X-ray photoelectron spectroscopy. Their mass, thickness, and enzyme-immobilization ability could be easily tuned by the initiator reaction time, monomer ratio, and polymerization time. The antibacterial activity and stability of the immobilized lysozymes were studied by fluorescent staining and bacteria lysis assay, which revealed that the lysozymes on the copolymer brushes had good stability during storage at 4 °C for up to 30 days.

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A Brief History of Polymeric Cryogels

Lozinsky

2014

Advances in Polymer Science

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Catalytic activity of bovine lactoperoxidase supported on macroporous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)

Cited by 8 publications

References 29 publications

Surfactant-Free Poly(styrene-co-glycidyl methacrylate) Particles with Surface-Bound Antibodies for Activation and Proliferation of Human T Cells

Surfactant-Free Poly(styrene-co-glycidyl methacrylate) Particles with Surface-Bound Antibodies for Activation and Proliferation of Human T Cells

Immobilization of Enzymes on 2‐Hydroxyethyl Methacrylate and Glycidyl Methacrylate Copolymer Brushes

A Brief History of Polymeric Cryogels

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