Immobilization of Enzymes on 2‐Hydroxyethyl Methacrylate and Glycidyl Methacrylate Copolymer Brushes

Abstract

The immobilization of enzymes is of paramount importance to maintain their activity and stability. In this study, surface-initiated atom-transfer radical polymerization was applied to prepare poly(2-hydroxyethyl methacrylate)-block-poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) brushes on glass slides. The polymerization kinetics was followed by using a quartz crystal microbalance with dissipation monitoring and ellipsometry in terms of mass and thickness growth, respectively. The surface chemical compositions of the obtained polymer brushes were characterized by X-ray photoelectron spectroscopy. Their mass, thickness, and enzyme-immobilization ability could be easily tuned by the initiator reaction time, monomer ratio, and polymerization time. The antibacterial activity and stability of the immobilized lysozymes were studied by fluorescent staining and bacteria lysis assay, which revealed that the lysozymes on the copolymer brushes had good stability during storage at 4 °C for up to 30 days.

A complementary density gradient of zwitterionic polymer brushes and NCAM peptides for selectively controlling directional migration of Schwann cells

“…3-(2-Bromoisobutyramido)propyl triethoxy silane (BES) was synthesized according to literature [38,39]. Alkynylated peptide, KHIFSDDSSEK-Pra (KHI, Pra: L-Cpropargylglycine), was synthesized by Sangon Biotech (Shanghai, China).…”

Hairy Particles with Immobilized Enzymes: Impact of Particle Topology on the Catalytic Activity

“…20,21 The same tendency was found by Shang et al, who also confirmed that the amount of covalently immobilized glucose oxidase increased with the increase in thickness of the grafted poly(acrylic acid). 22 Further examples for this correlation were shown by the groups of Mao 23 and Gao, 24 who immobilized trypsin and lysozyme onto poly-(glycidyl methacrylate)-based brushes.…”

Dual Responsive Surfaces Based on Host–Guest Interaction for Dynamic Mediation of Cell–Substrate Interaction and Cell Migration

“…To create the β‐CD/azobenzene based responsible surface, hydrophilic poly(2‐hydroxyethyl methacrylate)‐ block ‐poly(2‐hydroxyethyl methacrylate‐ co ‐glycidyl methacrylate) (PHEMA‐ b ‐P(HEMA‐ co ‐GMA)) brushes were prepared via surface‐initiated atom‐transfer radical polymerization (SI‐ATRP) (Table S1, Supporting Information). β‐CD was immobilized on the polymer brushes via amino‐epoxy reaction for hosting azobenzene conjugated endothelial cells (ECs) selective Gly‐Arg‐Glu‐Asp‐Val (azo‐REDV) peptides. The surface property and cell mobility is adjusted by addition of competing guest molecules, adamantanamine hydrochloride (Ama), and UV light irradiation ( Figure ).…”