A complementary density gradient of zwitterionic polymer brushes and NCAM peptides for selectively controlling directional migration of Schwann cells

Ren, Tanchen; Yu, Shan Ping; Mao, Zhengwei; Gao, Changyou

doi:10.1016/j.biomaterials.2015.03.052

Cited by 62 publications

(56 citation statements)

References 65 publications

(59 reference statements)

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Section: Resultscontrasting

confidence: 72%

“…This is likely due to the existence of competitive adhesion between ECs and SMCs, which reduces the adhered cell number but improves the selective ratio. Compared with the previous results on antifouling polymers and selective biomolecules, [24,42,43] the selective ratio is higher in this study due to the homogenous antifouling polymer brushes generated by the "grafting from" method, leading to a higher grafting density. [44][45][46] All these results prove that the gradient CAG peptides immobilized on the cell-resisting polymer brushes could provide a gradient adhesion sites for ECs, providing the necessary cues for directional cell migration.…”

Section: Selective Adhesion Of Ecs Over Smcscontrasting

confidence: 77%

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Selective Adhesion and Directional Migration of Endothelial Cells Guided by Cys‐Ala‐Gly Peptide Density Gradient on Antifouling Polymer Brushes

Gao

2019

Macromolecular Bioscience

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Selective adhesion and directional migration of endothelial cells (ECs) on biomaterials is critical to realize the rapid endothelialization. In this study, a Cys‐Ala‐Gly (CAG) peptide density gradient is generated on homogeneous cell‐resisting poly(2‐hydroxyethyl methacrylate‐co‐glycidyl methacrylate) brushes by immersing the brushes in a complementary gradient solution of CAG and competitive mercapto‐terminated methoxyl poly(ethylene glycol). The adhesion and spreading of smooth muscle cells (SMCs) is impaired effectively on the gradient surface. About six folds of adherent ECs over SMCs are achieved at the position (10 mm) of highest CAG density on the gradient surface in a co‐culture condition. Due to the gradient cues, ECs migrate fastest with the best directionality of 86.7% at the middle of the gradient, leading to the maximum net displacement as well.

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Section: Resultscontrasting

confidence: 72%

Section: Selective Adhesion Of Ecs Over Smcscontrasting

confidence: 77%

Selective Adhesion and Directional Migration of Endothelial Cells Guided by Cys‐Ala‐Gly Peptide Density Gradient on Antifouling Polymer Brushes

Gao

2019

Macromolecular Bioscience

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“…Regarding the main properties that these devices must have, the most important are: biocompatibility, biodegradability, permeability, biomechanical properties, surface properties, custom dimension and interaction with neurotrophic factors …”

Section: Ideal Nerve Guide Conduit Propertiesmentioning

confidence: 99%

Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials

Pinho

Fonseca

Serra

et al. 2016

Adv Healthcare Materials

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Experiments concerning peripheral nerve regeneration have been reported since the end of the 19th century. The need to implement an effective surgical procedure in terms of functional recovery has resulted in the appearance of several approaches to solve this problem. Nerve autograft was the first approach studied and is still considered the gold standard. Since autografts require donor harvesting, other strategies involving the use of natural materials have also been studied. Nevertheless, the results were not very encouraging and attention has moved towards the use of nerve conduits made from polymers, whose properties can be easily tailored and which allow the nerve conduit to be easily processed into a variety of shapes and forms. Some of these materials are already approved by the US Food and Drug Administration (FDA), as is presented here. Furthermore, polymers with conductive properties have very recently been subject to intensive study in this field, since it is believed that such properties have a positive influence in the regeneration of the new axons. This manuscript intends to give a global view of the mechanisms involved in peripheral nerve regeneration and the main strategies used to recover motor and sensorial function of injured nerves.

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“…52 In an earlier communication, we had successfully reported one kind of zwitterionic polyurethane for shape memory materials by two-step reactions. [54][55][56][57] In this experiment, a series of zwitterionic copolymers, called as p(DMAPS-co-AA), were synthesized from DMAPS and acrylic acid (AA). In the present study, we would like propose another strategy to prepare a new kind of multi-SMPs by a free radical polymerization method using a zwitterionic monomer, 3-dimethyl (methacryloyloxyethyl) ammonium propane sulfonate (DMAPS).…”

Section: Introductionmentioning

confidence: 99%

Development of zwitterionic copolymers with multi-shape memory effects and moisture-sensitive shape memory effects

Chen

Stadler

et al. 2015

J. Mater. Chem. B

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Shape memory polymers (SMP) and zwitterionic polymers both have great applications in biomedical fields. This works successfully combines functionalities of zwitterionic polymers and SMP, developing new kind of zwitterionic copolymers having a multi-shape memory effect (SME) and a moisture-sensitive SME. The results demonstrate that a series of zwitterionic multi-SMPs, coded as p(DMAPS-co-AA), were synthesized from DMAPS and acrylic acid (AA). A micro-phase separated structure is formed in the resulting p(DMAPS-co-AA). The strong hydrogen bonding between AA segments serves as a reversible switch, while the strong electrostatic forces among DMAPS segments serve as physical crosslinkers. Therefore, shape memory testing demonstrates that p(DMAPS-co-AA) shows not only dual-SME, but also triple-SME and quadruple-sSME. Moreover, in addition to the thermally-induced SME, p(DMAPS-co-AA) also shows moisture-sensitive SME. It is thus proposed that this zwitterionic multi-SMP could find great potential applications in smart biomedical fields. Fig. 8 Thermally-induced multi-SMEs of DMAPS-co-AA copolymers (A) repeated dual-SME; (B) triple-SME; (C) quadruple-SME; (D) sequential shape recovery; (E) a group of photos showing the sequential fixed at 110 1C; 90 1C and 70 1C; recovered at 90 1C and 110 1C.Fig. 9 Moisture absorption curves (A) and moisture-sensitive strain recovery curves (B) of DMAPS-co-AA copolymer with different DMAPS contents at 80% RH and T = 30 1C; (C) the moisture-sensitive shape recovery process of sample DMAPS50 at 80% RH and T = 30 1C ((a) 0 min; (b) 10 min; (c) 20 min; (d) 30 min; (e) 40 min; (f) 50 min; (g) 60 min; (h) 70 min; (i) 80 min). Journal of Materials Chemistry B Paper

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A complementary density gradient of zwitterionic polymer brushes and NCAM peptides for selectively controlling directional migration of Schwann cells

Cited by 62 publications

References 65 publications

Selective Adhesion and Directional Migration of Endothelial Cells Guided by Cys‐Ala‐Gly Peptide Density Gradient on Antifouling Polymer Brushes

Selective Adhesion and Directional Migration of Endothelial Cells Guided by Cys‐Ala‐Gly Peptide Density Gradient on Antifouling Polymer Brushes

Peripheral Nerve Regeneration: Current Status and New Strategies Using Polymeric Materials

Development of zwitterionic copolymers with multi-shape memory effects and moisture-sensitive shape memory effects

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