Catabolite control protein A is an important regulator of metabolism in Streptococcus suis type 2

Abstract

() type 2 is an extremely important Gram-positive bacterial pathogen that can cause human or swine endocarditis, meningitis, bronchopneumonia, arthritis and sepsis. Catabolite control protein A (CcpA) is a major transcriptional regulator in type 2 that functions in catabolite control, specifically during growth on glucose or galactose. The regulation of central metabolism can affect the virulence of bacteria. In the present study, a metabolomics approach was used along with principal components analysis (PCA) and partial least-squares-discriminant analysis (PLS-DA) models and 37 metabolites were found that differed substantially between native and a mutant lacking CcpA. These results showed that CcpA is an important protein in type 2 for studying bacterial protein function.

Investigation into the role of catabolite control protein A in the metabolic regulation of Streptococcus suis serotype 2 using gene expression profile analysis

“…According to the network database and the results of the present study, succinic, aspartic and citric acid concentrations decreased and affected the Streptococcus genome under the influence of the CcpA gene, which was verified by previous research from metabolite experiments (20). Furthermore, downregulation of ssu05_1508, ssu05_1065, ssu05_1164, ssu05_1640 and ssu05_2154, and upregulation of ssu05_1885 and ssu05_1839 was observed (Fig.…”

“…Based on previous studies of associated gene metabonomics, the results demonstrated that CcpA affected carbon metabolism to a certain extent by connecting differential metabolites, genes and proteins (20). According to the network database and the results of the present study, succinic, aspartic and citric acid concentrations decreased and affected the Streptococcus genome under the influence of the CcpA gene, which was verified by previous research from metabolite experiments (20).…”

“…S. suis 2 and CcpA mutant strains (20) were cultivated to a logarithmic phase in Todd-Hewitt broth medium (Oxoid Ltd., Basingstoke, UK) at 37˚C in an atmosphere of 5% CO 2 . The strains were stored in our laboratory at the Institute of Military Veterinary (Changchun, China) until required for further use.…”

“…In order to further elucidate the role of CcpA in the metabolic regulation of S. suis 2, the effects of CcpA on regulation mechanisms were investigated using gene expression profiles based on previous metabonomic analyses (20). In addition, KEGG network data were used to establish a network diagram of interactions among metabolites, genes and proteins.…”

Catabolite control protein A has an important role in the metabolic regulation of Streptococcus suis type 2 according to iTRAQ-based quantitative proteomic analysis

“…The metabolomics analysis of the present study found that ccpA mutation caused changes in metabolites, including glutamate, guanine, uridine and inosine, which are primarily involved in the metabolism of amino acids, nucleic acids, fats and certain small-molecule organic acids. These results have been verified by a further metabolomics study by our group (19). A comparison of the data from the two studies indicated that ccpA affected overall pathogenicity, metabolites and virulence through different pathways (9,19).…”