Catabolite control protein A has an important role in the metabolic regulation of Streptococcus suis type 2 according to iTRAQ-based quantitative proteomic analysis

Lang, Xulong; Zhong-hai, Wan; Pan, Ying; ZhaoYang, Bu; Wang, Xiuran; Wang, Xiaoxu; Ji, Xue; Zhu, Li; Wan, Jiayu; Sun, Yansong; Wang, Xinglong

doi:10.3892/mmr.2015.4211

Cited by 2 publications

(2 citation statements)

References 25 publications

(29 reference statements)

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“…The group of Lang et al, applied gene expression profile analysis, metabolomics, as well as proteomics, to investigate the role of ccpA in S. suis. Their studies underlined an involvement of CcpA in sugar, amino acid, nucleic acid and fat metabolism as ccpA activity alters the concentration of certain metabolites [85,86]. A decrease in succinic, aspartic, and citric acid concentrations changed glucose availability and therefore, affected S. suis metabolism regulation [87].…”

Section: Catabolite Control Protein a (Ccpa)mentioning

confidence: 99%

Role of Metabolic Adaptation of Streptococcus suis to Host Niches in Bacterial Fitness and Virulence

Dresen¹,

Valentin‐Weigand²,

Weldearegay³

2023

Pathogens

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Streptococcus suis, both a common colonizer of the porcine upper respiratory tract and an invasive pig pathogen, successfully adapts to different host environments encountered during infection. Whereas the initial infection mainly occurs via the respiratory tract, in a second step, the pathogen can breach the epithelial barrier and disseminate within the whole body. Thereby, the pathogen reaches other organs such as the heart, the joints, or the brain. In this review, we focus on the role of S. suis metabolism for adaptation to these different in vivo host niches to encounter changes in nutrient availability, host defense mechanisms and competing microbiota. Furthermore, we highlight the close link between S. suis metabolism and virulence. Mutants deficient in metabolic regulators often show an attenuation in infection experiments possibly due to downregulation of virulence factors, reduced resistance to nutritive or oxidative stress and to phagocytic activity. Finally, metabolic pathways as potential targets for new therapeutic strategies are discussed. As antimicrobial resistance in S. suis isolates has increased over the last years, the development of new antibiotics is of utmost importance to successfully fight infections in the future.

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Section: Catabolite Control Protein a (Ccpa)mentioning

confidence: 99%

Role of Metabolic Adaptation of Streptococcus suis to Host Niches in Bacterial Fitness and Virulence

Dresen¹,

Valentin‐Weigand²,

Weldearegay³

2023

Pathogens

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“…Cells were randomly pooled into three groups. The three replicates were processed for iTRAQ analysis to identify differentially expressed proteins, as previously described with minor revisions (26,27). Briefly, each sample was sonicated for 15 min in 500 mL SDT lysis buffer (4% sodium dodecyl sulfate (SDS) and 100mM Tris-HCl, pH 7.6).…”

Section: Protein Preparation and Digestionmentioning

confidence: 99%

Proteomic Analysis Reveals That Placenta-Specific Protein 9 Inhibits Proliferation and Stimulates Motility of Human Bronchial Epithelial Cells

et al. 2021

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Placenta-specific protein 9 (PLAC9) is a putative secretory protein that was initially identified in the placenta and is involved in cell proliferation and motility. Bioinformatics analyses revealed that PLAC9 is repressed in lung cancers (LCs), especially lung adenocarcinomas, compared to that in the paired adjacent normal tissues, indicating that PLAC9 might be involved in the pathogenesis of pulmonary diseases. To investigate the potential role of PLAC9 in the abnormal reprogramming of airway epithelial cells (AECs), a key cause of pulmonary diseases, we constructed a stable PLAC9-overexpressing human bronchial epithelial cell line (16HBE-GFP-Plac9). We utilized the proteomic approach isobaric tag for relative and absolute quantification (iTRAQ) to analyze the effect of PLAC9 on cellular protein composition. Gene ontology (GO) and pathway analyses revealed that GO terms and pathways associated with cell proliferation, cell cycle progression, and cell motility and migration were significantly enriched among the proteins regulated by PLAC9. Our in vitro results showed that PLAC9 overexpression reduced cell proliferation, altered cell cycle progression, and increased cell motility, including migration and invasion. Our findings suggest that PLAC9 inhibits cell proliferation through S phase arrest by altering the expression levels of cyclin/cyclin-dependent kinases (CDKs) and promotes cell motility, likely via the concerted actions of cyclins, E-cadherin, and vimentin. Since these mechanisms may underlie PLAC9-mediated abnormal human bronchial pathogenesis, our study provides a basis for the development of molecular targeted treatments for LCs.

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Catabolite control protein A has an important role in the metabolic regulation of Streptococcus suis type 2 according to iTRAQ-based quantitative proteomic analysis

Cited by 2 publications

References 25 publications

Role of Metabolic Adaptation of Streptococcus suis to Host Niches in Bacterial Fitness and Virulence

Role of Metabolic Adaptation of Streptococcus suis to Host Niches in Bacterial Fitness and Virulence

Proteomic Analysis Reveals That Placenta-Specific Protein 9 Inhibits Proliferation and Stimulates Motility of Human Bronchial Epithelial Cells

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