Investigation into the role of catabolite control protein A in the metabolic regulation of Streptococcus suis serotype 2 using gene expression profile analysis

Abstract

Abstract.Catabolite control protein A (CcpA) serves a key function in the catabolism of Streptococcus suis serotype 2 (S. suis 2) by affecting the biological function and metabolic regulatory mechanisms of this bacterium. The aim of the present study was to identify variations in CcpA expression in S. suis 2 using gene expression profile analysis. Using sequencing and functional analysis, CcpA was demonstrated to play a regulatory role in the expression and regulation of virulence genes, carbon metabolism and immunoregulation in S. suis 2. Gene Ontology and Kyto Encyclopedia of Genes and Genomes analyses indicated that CcpA in S. suis 2 is involved in the regulation of multiple metabolic processes. Furthermore, combined analysis of the transcriptome and metabolite data suggested that metabolites varied due to the modulation of gene expression levels under the influence of CcpA regulation. In addition, metabolic network analysis indicated that CcpA impacted carbon metabolism to a certain extent. Therefore, the present study has provided a more comprehensive analysis of the role of CcpA in the metabolic regulation of S. suis 2, which may facilitate future investigation into this mechanism. Furthermore, the results of the present study provide a foundation for further research into the regulatory function of CcpA and associated metabolic pathways in S. suis 2.

Catabolite control protein A has an important role in the metabolic regulation of Streptococcus suis type 2 according to iTRAQ-based quantitative proteomic analysis

“…These results have been verified by a further metabolomics study by our group (19). A comparison of the data from the two studies indicated that ccpA affected overall pathogenicity, metabolites and virulence through different pathways (9,19). The altered functions of various metabolic proteins, including peptidoglycan glycosyltransferase, enolase, sortase, GAPDH, 6-phosphogluconate dehydrogenase and glutamine synthetase may have affected the virulence of the bacteria, which was also reported by a previous study (20).…”

Transcriptome, Phenotypic, and Virulence Analysis of Streptococcus sanguinis SK36 Wild Type and Its CcpA-Null Derivative (ΔCcpA)

“…To characterize the GO terms, including molecular functions, biological processes, cellular components, and functional pathways of DEGs, significantly enriched GO terms was analyzed by a hypergeometric test, based on “GO Term Finder” (Boyle et al, 2004; Lang et al, 2015). All the DEGs were mapped to the terms in KO (KEGG Orthology) identifier (Kanehisa et al, 2012; Zeng et al, 2013) using KOBAS 2.0 to identify which pathways the significant DEGs belonged to.…”

Role of Metabolic Adaptation of Streptococcus suis to Host Niches in Bacterial Fitness and Virulence

“…Their studies underlined an involvement of CcpA in sugar, amino acid, nucleic acid and fat metabolism as ccpA activity alters the concentration of certain metabolites [85,86]. A decrease in succinic, aspartic, and citric acid concentrations changed glucose availability and therefore, affected S. suis metabolism regulation [87].…”

“…HPr an important co-factor of this binding is phosphorylated by the protein HP0197 [95]. CcpA is involved in diverse metabolic pathways, the expression of virulence factors and certain enzymes such as the α-galactosidase [13,24,84,85,87]. In addition, it takes part in glycolysis and galactose utilization.…”