Case Report: Subconjunctival Injection With Autologous Platelet-Rich Plasma for Refractory Corneal Ulcers in a Dog

Abstract

A 12-year-old, intact, male Golden Retriever dog presented with a 1-month history of corneal ulcer, redness, and occasional squint in the left eye (oculus sinister [OS]). A thorough ophthalmological examination revealed corneal edema, diffuse corneal vascularization, and excessive granulation tissue growth. The tentative diagnosis was chronic superficial keratitis or pannus. After 3 weeks of treatment for OS, blepharospasm and superficial corneal ulceration with a lip of nonadherent epithelium of the right eye (oculus dexter [OD]) developed. No improvement was observed after autologous serum eye drop treatment combined with simple debridement and grid keratotomy on both eyes (oculi uterque [OU]) for 3 months; thereafter, blindness developed. The diagnosis was refractory corneal ulcers. The application of autologous platelet-rich plasma (PRP) was instilled via subconjunctival injection OU every 2 weeks for 2 months. The OU corneas started to re-epithelialize and heal, and the granulation tissues significantly decreased while the vision improved. Therefore, PRP was continued to be administered every 1–2 months for 6 months. After more than 1 year, the OU corneas have healed without recurrent ulceration and with only mild haze. In conclusion, autologous PRP subconjunctival injection is a potentially beneficial addition to the medical management of refractory corneal ulcers in dogs.

Corneal Ulcer in Dogs and Cats: Novel Clinical Application of Regenerative Therapy Using Subconjunctival Injection of Autologous Platelet-Rich Plasma

“…A subconjunctival route was chosen to administer PRP in both dogs and cats. In two reports dealing with rabbits and dogs, the subconjunctival injection was used instead of drops and can be given as a single shot contrary to the drop form ( 5 , 52 ).…”

Use of Platelet Rich Plasma in Veterinary Surgery: Systematic Review

“…[30][31][32] Kornea ülseri olan ve tedaviden sonuç alınamayan bir köpekte, ilk TZP enjeksiyonundan 1 ay sonra her 2 gözde ülserlerin iyileşmeye başladığı, 2 ay sonra her iki korneada daha belirgin iyileşme görüldüğü ve granülasyon dokularının önemli ölçüde kaybolduğu, bununla birlikte görmede de iyileşme olduğu, 8 ay sonra ise her iki gözde de sadece küçük sekeller kaldığı bildirilmiştir. 33 Yine kedi ve köpeklerde, kornea ülserinde otolog TZP'nin subkonjonktival enjeksiyonunun kullanıldığı bir çalışmada, ülser tipi belirlendikten sonra TZP enjeksiyonu uygulanmıştır. Kedilerde tam iyileşme sağlanana kadar yüzeysel tek taraflı ve orta stromal ülserli vakaların çoğunda 2 TZP enjeksiyonuna, derin (descemetosel) ülserli olgularda 3 enjeksiyona ve eriyen tip ülserli olgularda ise 4 enjeksiyona ihtiyaç duyulduğu, köpeklerde ise iyileşme için olguların %50'sinde 2 TZP enjeksiyonunun yeterli olduğu bildirilmiştir.…”

Effect of Platelet Rich Plasma on Corneal Alkali Burn Ulcer in Rabbits

“…It should be noted that the blood sample in our investigation had to be collected intracardiacally as (Gimeno et al,, 2006) because we were not able to avoid blood coagulation by using different puncture sites, such as the ear or femoral vein. However, some authors collect blood from ear vein as (Acosta et al, 2014) and from jugular vein as (Yang et al,, 2018).…”