Objective of this study to assess the efficacy of platelet rich plasma (PRP) in the treatment of extensive corneal ulcers in albino rabbits with two different administration techniques. A total of 24 rabbits were used in this study. After collecting intracardiac blood samples, platelet-rich plasma was obtained by double centrifugation. Alkali burns were inflicted on the central corneas of all rabbits using a sterile swab soaked in 1 M NaOH for 60s. Only Left eye in each rabbit was used. Animals were divided into 4 groups. GroupI (n=6) normal control group received PRP drop twice daily with 8-h intervals for 7 successive days. whereas group II (n = 6) did not receive any treatment and served as the control untreated group. The third group (n = 6) received only oneshot of subconjunctival PRP. The fourth group (n=6) received PRP drop twice daily with 8-h intervals for 7 successive days. Corneal histopathology was investigated for epithelial regeneration, presence of inflammation, and structural integrity of fibroblasts. Data were expressed as mean ± standard deviations. Data from experimental groups were statically analyzed using one-way ANOVA with Turkey's post hoc multiple comparisons tests using the GraphPad Prism software version 5 (San Diego, CA, USA) and P<0.05 was used to define statistically significant differences between the groups. Group III had a significantly high rate (P<0.05) of corneal epithelial healing as compared with the control group. Group IV had moderate restoration of corneal epithelium defects. Group III had a significantly better healing process after the fifth day as compared with group IV. Histopathological investigations revealed a steady fibroblast migration, quicker epithelial regeneration, and less inflammation in group III as compared with the other treated groups. A single dose of subconjunctival PRP injection had an effective alleviating effect on alkali-induced corneal burn rather than PRP drops
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