“…The isothermal amplified nucleic acid detection has attracted substantial research interests, 1 – 3 and numerous isothermal amplification means have been developed and applied in homogeneous diagnostics, forensics, medicine, and agriculture. 4 – 6 These include nucleic acid sequence-based amplification (NASBA), 7 , 8 signal-mediated amplification of RNA technology (SMART), 9 , 10 rolling circle amplification (RCA), 11 , 12 strand displacement amplification (SDA), 13 , 14 and loop-mediated isothermal amplification (LAMP). 15 , 16 Isothermal amplification provides a rapid and efficient tool at a constant temperature without thermocycling required in traditional polymerase chain reaction (PCR).…”