Cartilage repair using mesenchymal stem cell (MSC) sheet and MSCs-loaded bilayer PLGA scaffold in a rabbit model

Qi, Yiying; Du, Yi; Li, Weixu; Dai, Xuesong; Zhao, Tengfei; Yan, Wei

doi:10.1007/s00167-012-2256-3

Cited by 74 publications

(65 citation statements)

References 39 publications

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“…A cell sheet was fabricated as we reported previously22. Briefly, confluent cells in flasks at 1 × 10 5 /cm 2 were cultured in L-DMEM with the addition of vitamin C (20 μg/mL)23 for 2 weeks until a sheet of rBMSCs formed and could be detached intact from the substratum using a cell scraper (Supplemental material 2).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

Zhang

Xue

Yin

et al. 2016

Sci Rep

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HSPA1A, which encodes cognate heat shock protein 70, plays important roles in various cellular metabolic pathways. To investigate its effects on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), its expression level was compared between undifferentiated and differentiated BMSCs. Rat HSPA1A overexpression in BMSCs increased osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. Moreover, it upregulated β-catenin and downregulated DKK1 and SOST. The enhanced osteogenesis due to HSPA1A overexpression was partly rescued by a Wnt/β-catenin inhibitor. Additionally, using a rat tibial fracture model, a sheet of HSPA1A-overexpressing BMSCs improved bone fracture healing, as determined by imaging and histological analysis. Taken together, these findings suggest that HSPA1A overexpression enhances osteogenic differentiation of BMSCs, partly through Wnt/β-catenin.

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Section: Methodsmentioning

confidence: 99%

Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

Zhang

Xue

Yin

et al. 2016

Sci Rep

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“…Qi et al (32) investigated the effects of incorporating the properties of MSC sheets into MSC-loaded bilayer poly-(lactic-co-glycolic acid) (PLGA) scaffolds to improve the integration and repair of cartilage defects in a rabbit model. At 6 and 12 weeks after implantation, the MSC sheet-encapsulated PLGA/MCSs group showed significantly more hyaline cartilage and higher histological scores compared with the other groups.…”

Section: Msc Sheets For Cartilage Integration and Repairmentioning

confidence: 99%

Application of the cell sheet technique in tissue engineering

Chen

Niu

et al. 2015

Biomedical Reports

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“…Besides that, MSCs have been recently exploited to develop continuous cell sheets as an alternative strategy to benefit stem cell-based tissue engineering, such as blood vessels, 18 cartilage, 19 bone, 20 and myocardial. 21 To explore the effect of different cues (e.g.…”

Section: Introductionmentioning

confidence: 99%

The effects of poly(dimethylsiloxane) surface silanization on the mesenchymal stem cell fate

et al. 2015

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In recent years, poly(dimethylsiloxane) (PDMS)-based microfluidic devices have become very popular for on-chip cell investigation. Maintenance of mammalian cell adhesion on the substrate surface is crucial in determining the cell viability, proliferation and differentiation. However, the inherent hydrophobicity of PDMS is unfavourable for cell culture, causing cells to eventually dislodge from the surface. Although physically adsorbed matrix proteins can promote initial cell adhesion, this effect is usually short-lived. To address this critical issue, in this study, we employed (3-aminopropyl) triethoxy silane (APTES) and cross-linker glutaraldehyde (GA) chemistry to immobilize collagen type 1 (Col1) on PDMS. These modified surfaces are highly efficient to support the adhesion of mesenchymal stem cells (MSCs) with no deterioration of their potency. Significant changes of the native PDMS surface properties were observed with the proposed surface functionalization, and MSC adhesion was improved on PDMS surfaces modified with APTES + GA + Protein. Therefore, this covalent surface modification could generate a more biocompatible platform for stabilized cell adhesion. Furthermore, this modification method facilitated long-term cell attachment, which is favourable for successful induction of osteogenesis and cell sheet formation with an increased expression of osteogenic biomarkers and comparable extracellular matrix (ECM) constituent biomarkers, respectively. The surface silanization can be applied to PDMS-based microfluidic systems for long-term study of cellular development. Similar strategies could also be applied to several other substrate materials by appropriate combinations of self-assembled monolayers (SAMs) and ECM proteins.

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Cartilage repair using mesenchymal stem cell (MSC) sheet and MSCs-loaded bilayer PLGA scaffold in a rabbit model

Cited by 74 publications

References 39 publications

Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

Application of the cell sheet technique in tissue engineering

The effects of poly(dimethylsiloxane) surface silanization on the mesenchymal stem cell fate

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