Application of the cell sheet technique in tissue engineering

Chen, Guangnan; Qi, Yiying; Niu, Lie; Di, Tuoyu; Zhong, Jinwei; Fang, Tingting; Yan, Wei

doi:10.3892/br.2015.522

Cited by 73 publications

(59 citation statements)

References 59 publications

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“…While this review focuses on MSCs, significant progress has been made using other stem cells, such as induced pluripotent stem cells (iPSCs) and embryonic stem cells . For example, iPSCs are a promising cell source, owing to its multipotent differentiation potential and ability to be passaged indefinitely .…”

Section: Therapeutic Approaches Using Tspcs and Other Mscsmentioning

confidence: 99%

Mesenchymal stem cells in tendon repair and regeneration: basic understanding and translational challenges

Leong

Sun

2016

Annals of the New York Academy of Sciences

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Tendon injuries are common and present a clinical challenge because they often respond poorly to treatment and require prolonged rehabilitation. Current treatments often do not completely repair or regenerate the injured or diseased tendon to its native composition, structure, and mechanical properties. Stem cell-based therapies have brought new hope for tissue repair and regeneration, including that for tendon rupture and tendinopathy. Despite tremendous effort and progress, the success of stem cell-based studies on tendon repair and regeneration has mainly been limited to preclinical studies with few clinical applications. In this concise review, we discuss basic understanding and translational challenges of using mesenchymal stem cells (MSCs) for tendon repair and regeneration, with a focus on (1) tendon stem/progenitor cells (TSPCs) and therapeutic approaches using TSPCs and other MSCs, (2) regulation of fate determination in MSCs for tendon-lineage differentiation, (3) pretreatment and condition of stem/progenitor cells for transplantation, and (4) a treatment approach that involves stimulating endogenous stem cells to enhance tendon healing. The review concludes with discussion on future directions.

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Section: Therapeutic Approaches Using Tspcs and Other Mscsmentioning

confidence: 99%

Mesenchymal stem cells in tendon repair and regeneration: basic understanding and translational challenges

Leong

Sun

2016

Annals of the New York Academy of Sciences

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“…Therefore, the possible application of an artificial cornea with the aid of corneal tissue engineering has brought hope to patients with corneal blindness. This would be a feasible purpose if several criteria such as biocompatibility, stability of the tissue engineering constructs and suitability of the cell source in the development of the artificial cornea were to be acquired …”

Section: Inroductionmentioning

confidence: 99%

Conjunctiva derived mesenchymal stem cell (CJMSCs) as a potential platform for differentiation into corneal epithelial cells on bioengineered electrospun scaffolds

Soleimanifar

Mortazavi

Nadri

et al. 2017

J Biomedical Materials Res

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With the aid of tissue engineering, the capability of nanofibrous scaffolds was examined for corneal epithelial differentiation of Conjunctiva mesenchymal stem cells (CJMSCs) as a new source. This study tried to provide a synthetic corneal sheet which could be applied in corneal regeneration. The hybrid arrangement of fine Silk fibers with the diameter of 93.65 nm and the polyurethane (PU) fibers with an average diameter of 575.5 makes a mat with sufficient mechanical properties and interconnected pores that could accelerate nutrient diffusion. After methanol and plasma treatment, the highest biocompatibility of Silk-PU hybrid nanofibrous scaffolds based on cell attachment and proliferation were proven using MTT assay. The differentiation morphology of cells was observed by SEM micrographs of cells seeded on the scaffold after 12 days treatment through the differentiation medium. Despite up-regulation of Cytokeratin (CK)3, CK8, CK12, Desmocollin (DSC)1, and Desmoglein (DSG)1 in the treated PU and silk hybrid nanofibrous scaffolds, the relative expression of CK3, CK8, and CK12 genes were highest among TCP, treated PU and silk nanofibrous scaffolds. Immunocytochemistry (ICC) analysis was used to characterize the protein expression of CK3 in CJ-MSCs seeded on different groups. Although, For both TCP and hybrid group, CJ-MSCs were positive for corneal epithelial-specific marker CK3, ICC data demonstrated that CJ-MSCs seeded on the hybrid group likewise PU or Silk pure nanofibrous scaffolds, could differentiate into corneal epithelial-like cells. Finally, it was inferred that the hybrid PU and silk nanofibrous scaffold has the potential for the treatment of corneal epithelium. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2703-2711, 2017.

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“…141,142,143 However, cell sheets also suffer from certain disadvantages. In addition to low physical load bearing capacity, a multilayered cell sheet may lead to cell necrosis due to poor nutrition or hypoxia in the middle layers.…”

Section: Alternative Bottom-up Approachesmentioning

confidence: 99%

“…Hypoxia pretreatment can potentially increase the survival rate of implanted MSCs and may promote angiogenesis in vivo. 142 Nevertheless, cell sheet techniques may be used either alone or in combination with scaffold materials to generate implants that exhibit osteogenic and vascularization capabilities. 144 For example, an MSC cell sheet technique may enhance cell-cell and cell-scaffold interactions by promoting osteoblasts attachment to the mineralized layered cell sheet and may mimic the in vivo deposition of bone matrix.…”

Section: Alternative Bottom-up Approachesmentioning

confidence: 99%

“…Another example of combining top-down and bottom-up approaches is cell sheet-scaffold hybrid techniques to bone tissue regeneration that have recently been reviewed by Chen et al 142 For example, Ouyang et al revitalized cryopreserved demineralized bone grafts (top-down approach) by wrapping them with human MSC cell sheets (a bottom-up approach) and culturing for 3 weeks. The MSC cell sheet in this set up was analogous to periosteum and could be differentiated into the osteochondral lineage.…”

Section: Hybrid Approaches For Bte Scaffold Modificationmentioning

confidence: 99%

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Approaches for building bioactive elements into synthetic scaffolds for bone tissue engineering

Kesireddy

Kasper

2016

J. Mater. Chem. B

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Bone tissue engineering (BTE) is emerging as a possible solution for regeneration of bone in a number of applications. For effective utilization, BTE scaffolds often need modifications to impart biological cues that drive diverse cellular functions such as adhesion, migration, survival, proliferation, differentiation, and biomineralization. This review provides an outline of various approaches for building bioactive elements into synthetic scaffolds for BTE and classifies them broadly under two distinct schemes; namely, the top-down approach and the bottom-up approach. Synthetic and natural routes for top-down approaches to production of bioactive constructs for BTE, such as generation of scaffold-extracellular matrix (ECM) hybrid constructs or decellularized and demineralized scaffolds, are provided. Similarly, traditional scaffold-based bottom-up approaches, including growth factor immobilization or peptide-tethered scaffolds, are provided. Finally, a brief overview of emerging bottom-up approaches for generating biologically active constructs for BTE is given. A discussion of the key areas for further investigation, challenges, and opportunities is also presented.

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Application of the cell sheet technique in tissue engineering

Cited by 73 publications

References 59 publications

Mesenchymal stem cells in tendon repair and regeneration: basic understanding and translational challenges

Mesenchymal stem cells in tendon repair and regeneration: basic understanding and translational challenges

Conjunctiva derived mesenchymal stem cell (CJMSCs) as a potential platform for differentiation into corneal epithelial cells on bioengineered electrospun scaffolds

Approaches for building bioactive elements into synthetic scaffolds for bone tissue engineering

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