Cartilage formation through alterations of amphiphilicity of poly(ethylene glycol)–poly(caprolactone) copolymer hydrogels

Ko, Chao-Yin; Yang, Chan-Shan; Yang, Shang‐Dong; Ku, Kuan-Lin; Tsao, Chung‐Kan; Chuang, David Chwei‐Chin; Chu, I‐Ming; Cheng, Ming‐Huei

doi:10.1039/c3ra42406e

Cited by 19 publications

(18 citation statements)

References 48 publications

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“…The ACs continuously produced proteoglycans after the additional 4 weeks of in vitro or in vivo cultivation. This was similar to the results obtained by Ko et al (2013;, who demonstrate that the rabbit knee articular chondrocytes embedded in a 10 % PEG/PCL hydrogel express chondrogenic genes and produce GAGs after 2 weeks of in vitro cultivation. The two types of chondrocytes, auricular and articular, isolated from the elastic and hyaline cartilage, respectively, sustain their chondrogenic phenotype in the PEG/PCL hydrogel, suggesting that the PEG/PCL hydrogel is an ideal matrix for cartilage formation.…”

Section: Discussionsupporting

confidence: 91%

“…The PEG/PCL co-polymer was synthesised by ringopening polymerisation based on a previous study by Ko et al (2013). Briefly, PEG (molecular weight = 3,350 g/mol, Sigma-Aldrich) and PCL (molecular weight = 968 g/mol, Sigma-Aldrich) were stirred at 100 °C.…”

Section: Synthesis Of the Peg/pcl Co-polymermentioning

confidence: 99%

“…Park et al (2007) successfully cultivate rabbit knee articular chondrocytes into neocartilage using a PEG/PCL-based scaffold under both in vitro and in vivo conditions. Further, Ko et al (2013) encapsulate articular chondrocytes within a PEG/PCL hydrogel and reveal that the formation of hydrophilic and hydrophobic regions in the PEG/ PCL hydrogel promotes chondrocyte aggregation, leading to increased production and accumulation of collagen type II. They show a successful repair of a rabbit knee cartilage defect by injecting articular chondrocytes and bone marrow mesenchymal stem cells (BMSCs) with a PEG/PCL hydrogel (Ko et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Cultivation of auricular chondrocytes in poly(ethylene glycol)/poly(ε-caprolactone) hydrogel for tracheal cartilage tissue engineering in a rabbit model

Chang¹,

Yang²,

Hsiao³

et al. 2018

eCM

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Tissue engineering has the potential to overcome the limitations of tracheal reconstruction. To tissue-engineer a tracheal cartilage, auricular chondrocytes were encapsulated in a photocurable poly(ethylene glycol)/poly(ε-caprolactone) (PEG/PCL) hydrogel. Chondrogenic genes, including Sox9, Acan and Col2a1, were up-regulated in auricular chondrocytes after 2 weeks of in vitro cultivation in the PEG/PCL hydrogel. Co-cultivation of 70 % auricular chondrocytes and 30 % bone marrow mesenchymal stem cells (BMSCs) accelerated the chondrogenic genes' expression in the PEG/PCL hydrogel. Cartilaginous matrix markers, including proteoglycans and collagen type II, were detected in the chondrocytes-encapsulated PEG/PCL hydrogel after 4 weeks of in vitro cultivation. The higher expression level of cartilaginous matrix markers was observed in the PEG/PCL hydrogel with co-cultivation of 70 % chondrocytes and 30 % BMSCs. After 4 weeks of ectopic cultivation in rabbits, the cylindrical PEG/PCL structure was sustained with the use of a luminal silicon stent. However, without the stent, the construct collapsed under a compression force. No fibrosis or vessel ingrowth were found in the PEG/PCL hydrogel after 4 weeks of ectopic cultivation, whereas the auricular chondrocytes showed proteoglycans' accumulation and collagen type II production. Rabbit auricular chondrocytes could survive and retain chondrogenic ability in the PEG/PCL hydrogel under both in vitro and in vivo conditions. While the PEG/PCL hydrogel did not show sufficient mechanical properties for supporting the cylindrical shape of the construct, the high chondrogenesis level of chondrocytes in the PEG/PCL hydrogel displayed the potential of this material for tracheal tissue engineering.

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Section: Discussionsupporting

confidence: 91%

Section: Synthesis Of the Peg/pcl Co-polymermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cultivation of auricular chondrocytes in poly(ethylene glycol)/poly(ε-caprolactone) hydrogel for tracheal cartilage tissue engineering in a rabbit model

Chang¹,

Yang²,

Hsiao³

et al. 2018

eCM

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“…Copolymerization of non-degradable hydrophilic macromers with degradable lactide and glycolide blocks has been used to impart degradability and control the water content of PEG hydrogels [4,66] but solubility of the copolymer in the aqueous gel precursor solution for cell encapsulation decreased dramatically with increasing the length of lactide blocks [53]. Hydrogels synthesized from PEG and -caprolactone co-polymers are shown to be hydrolytically degradable, but the degradation rate is limited by the hydrophobicity and phase separation of -caprolactone segments in solution [67]. In addition to poly(aliphatic hydroxy acids), other hydrolytically degradable polymers including poly(ester amides) [68], polyphosphoesters [69,70], poly(amino-ester urethanes) [71] are used to synthesize degradable hydrogels.…”

Section: Degradation Of Micellar Gelsmentioning

confidence: 99%

Gelation characteristics, physico-mechanical properties and degradation kinetics of micellar hydrogels

Moeinzadeh

Jabbari

2015

European Polymer Journal

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Due to their high water content and diffusivity of nutrients and biomolecules, hydrogels are very attractive as a matrix for growth factor immobilization and in situ delivery of cells to the site of regeneration in tissue engineering. The formation of micellar structures at the nanoscale in hydrogels alters the spatial distribution of the reactive groups and affects the rate and extent of crosslinking and mechanical properties of the hydrogel. Further, the degradation rate of a hydrogel is strongly affected by the proximity of water molecules to the hydrolytically degradable segments at the nanoscale. The objective of this review is to summarize the unique properties of micellar hydrogels with a focus on our previous work on star polyethylene glycol (PEG) macromonomers chain extended with short aliphatic hydroxy acid (HA) segments (SPEXA hydrogels). Micellar SPEXA hydrogels have faster gelation rates and higher compressive moduli compared to their non-micellar counterpart. Owing to their micellar structure, SPEXA hydrogels have a wide range of degradation rates from a few days to many months as opposed to non-degradable PEG gels while both gels possess similar water contents. Furthermore, the viability and differentiation of mesenchymal stem cells (MSCs) is enhanced when the cells are encapsulated in degradable micellar SPEXA gels compared with those cells encapsulated in non-micellar PEG gels.

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“…The MMP sensitive PEG gel enhanced migration of fibroblasts encapsulated in the hydrogel compared to the non-degradable PEG gel [128]. Hydrogels based on copolymers of PEG and Ɛ-caprolactone were shown to be hydrolytically degradable but the rate of degradation was limited by the hydrophobicity and phase separation of Ɛ-caprolactone segments in aqueous solution [130]. Copolymerization of PEG with a degradable polymer like poly(lactide) (PLA) has been used to impart degradability to PEG hydrogels.…”

Section: Degradationmentioning

confidence: 99%

Hydrogels for Cell Encapsulation and Bioprinting

Shariati

Moeinzadeh

Jabbari

2015

Bioprinting in Regenerative Medicine

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Resumo: An in vitro encapsulation platform utilizing hydrogels and bone matrix (BM) scaffolds to investigate the effects of microenvironmental parameters on encapsulated goat mesenchymal stem cells (gMSC) was presented. The base encapsulation matrix was composed of a biocompatible hydrogel formed through a photoinitiated polymerization process. Different polymer concentrations were used to compare the effects of hydrogel crosslinking density on physical properties, as well as on cell viability. The potential of BM to support the growth and differentiation of gMSC was also analyzed. Both methods were compared in order to analyze viability. Structures that better allow flow of oxygen showed more promising results, whereas BM structures require a better evaluation method for concrete results.

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Cartilage formation through alterations of amphiphilicity of poly(ethylene glycol)–poly(caprolactone) copolymer hydrogels

Cited by 19 publications

References 48 publications

Cultivation of auricular chondrocytes in poly(ethylene glycol)/poly(ε-caprolactone) hydrogel for tracheal cartilage tissue engineering in a rabbit model

Cultivation of auricular chondrocytes in poly(ethylene glycol)/poly(ε-caprolactone) hydrogel for tracheal cartilage tissue engineering in a rabbit model

Gelation characteristics, physico-mechanical properties and degradation kinetics of micellar hydrogels

Hydrogels for Cell Encapsulation and Bioprinting

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