Carnosic acid nanoparticles suppress liver ischemia/reperfusion injury by inhibition of ROS, Caspases and NF-κB signaling pathway in mice

Li, Hui; Sun, Jianjun; Chen, Guoyong; Wang, Weiwei; Xie, Zhantao; Tang, Gaofeng; Wei, Sha Xin

doi:10.1016/j.biopha.2016.04.064

Cited by 46 publications

(25 citation statements)

References 36 publications

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“…In the present study, phlorizin played an inhibitory role in decreasing the H 2 O 2 levels in a dose‐dependent manner (Table ). This phenomenon was similar to a previous finding that carnosic acid nanoparticles could suppress the liver ischemia/reperfusion injury by inhibiting ROS, caspases, and NF‐κB signaling pathway in mice (Li et al, ).…”

Section: Discussionsupporting

confidence: 91%

Apple phlorizin supplementation attenuates oxidative stress in hamsters fed a high-fat diet

et al. 2017

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Phlorizin from apple peel has been used as a nutrient for more than 100 years. The present study investigated the effects of phlorizin on the antioxidant capacity and the antioxidative mechanisms in hamsters fed high-fat diet supplemented with phlorizin (0.3, 0.6, and 0.9%). Results showed that phlorizin increased the antioxidant enzyme activities and reduced the malondialdehyde content in plasma, liver, heart, and kidney that might arise from the higher mRNA levels of CuZn-superoxide dismutase, Mn-superoxide dismutase (Mn-SOD), catalase, glutathione peroxidase, nuclear factor erythroid 2-related factor 2-like (Nrf2), and the protein expression of Mn-SOD (p < .05). Diet supplemented with phlorizin also significantly decreased the total cholesterol and triglycerides levels in plasma. The present study demonstrated that phlorizin might reduce lipid peroxidation and protect against cardiovascular issues in vivo. Practical applicationsPhlorizin, from apple peel, has been used as a nutrient for over 100 years. To date, despite extensive research on phlorizin, a report on its effect on lipid peroxidation, the antioxidant system in plasma, heart, liver, and kidney of hamsters fed high-fat diet is yet lacking. This report demonstrates that phlorizin can reduce lipid peroxidation and exert a protective effect on cardiovascular issues in vivo, which is valuable in the rational utilization of phlorizin in functional foods. K E Y W O R D Santioxidant capacity, cholesterol, hamsters, phlorizin

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Section: Discussionsupporting

confidence: 91%

Apple phlorizin supplementation attenuates oxidative stress in hamsters fed a high-fat diet

et al. 2017

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“…Any injury to the cellular membrane of hepatocytes, such as I/R-induced hepatic injury, may result in a rapid increase of the serum ALT level (16). If the hepatic injury persists, AST expression in the mitochondria and circulation would be increased (17).…”

Section: Discussionmentioning

confidence: 99%

Role of mitofusin 2 in the protective effect of breviscapine against hepatic ischemia/reperfusion injury in rats

et al. 2018

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Abstract. The purpose of the present study was to investigate the effect of breviscapine injection on hepatic ischemia/reperfusion (I/R) injury in rats. To explore the relevance and discuss the underlying mechanism of mitofusin 2 (Mfn2) in hepatic I/R injury, 40 Sprague-Dawley male rats were randomly and equally divided into five groups (n=8 per group) as follows: Sham, I/R + normal saline 1 (NS1), I/R + breviscapine 1 (Bre1), I/R + NS2 and I/R + Bre2 groups. Groups 1 and 2 represented ischemia for 20 and 60 min, respectively. Breviscapine or normal saline was injected via the tail vein (single dose of 10 mg/kg) 1 h prior to surgery and immediately postoperatively. The classical model of hepatic I/R injury was used in the present study. The blood and liver samples of different groups were collected following reperfusion to observe serum transaminases and histopathological changes. Alterations in Mfn2, cytosolic cytochrome c and cleaved caspase-3 were additionally assessed. The results demonstrated that breviscapine improved liver function, based on histopathological analysis, and decreased levels of the liver enzymes aspartate and alanine aminotransferase in the I/R + Bre groups compared with the I/R + NS group (P<0.05). The expression of Mfn2 was significantly increased in the I/R + Bre groups (P<0.05), whereas the expression of caspase-3 and cytosolic cytochrome c protein was decreased in the I/R + Bre groups (P<0.05) compared with the I/R + NS group. These data provided substantial evidence that breviscapine treatment exerted a protective effect against damage induced by hepatic I/R. This protective effect was possibly due to its ability to inhibit I/R-induced apoptosis and promote the expression of Mfn2.

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“…The level of ROS was determined by detecting the fluorescence intensity of the oxidant-sensitive probe 2,7-dichlorodihydrofluorescein diacetate (Molecular Probes; Thermo Fisher Scientific, Inc., Waltham, MA, USA) as described in a previous study ( 26 ). The amount of formed dichlorofluorescein in the clear supernatant was determined using a microplate reader (Infinite M200 PRO; Tecan, Zurich, Switzerland) at an excitation wavelength of 502 nm and an emission wavelength of 523 nm.…”

Section: Methodsmentioning

confidence: 99%

Hepatoprotective effect of methyl ferulic acid against carbon tetrachloride-induced acute liver injury in rats

Yang

et al. 2017

Exp Ther Med

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The present study aimed to investigate the hepatoprotective effects of methyl ferulic acid (MFA) against oxidative stress and apoptosis in acute liver injury induced by carbon tetrachloride (CCl4) in rats, as well as the underlying mechanisms. Sprague Dawley rats were treated with CCl4 after oral administration of MFA (25, 50, and 100 mg/kg) or dimethyl diphenyl bicarboxylate (200 mg/kg) for 7 days. The hepatoprotective effects of MFA were determined by analyzing serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities as well as changes of oxidant parameters. Histopathological analysis was performed to determine the degree of hepatic injury. The mechanisms were investigated by detecting the levels of NADPH oxidase (NOX) trans-membrane subunit NOX4, its ligand p22phox, as well as caspase3, cleaved caspase3, B-cell lymphoma (Bcl)-2, Bcl-2-associated X protein (Bax), tumor necrosis factor (TNF)-α, interleukin (IL)-1, reactive oxygen species (ROS), thiobarbituric acid-reactive substances (TBARS), total anti-oxidant capacity (TAC), phosphorylated J-Jun N-terminal kinase (p-JNK) and p-p38 mitogen-activated protein kinase (MAPK) using semi-quantitative polymerase chain reaction, western blot analysis and colorimetric assays. MFA treatment significantly decreased serum enzymatic activities of ALT and AST. MFA markedly increased activities of liver superoxide dismutase, catalase and glutathione peroxidase, and reduced the malondialdehyde concentration. Histopathological examination demonstrated that MFA reduced lipid degeneration, cytoplasmic vacuolization, necrosis and inflammatory cell infiltration in the liversof CCl4-treated rats. MFA treatment markedly inhibited the expression of inflammatory factors TNF-α and IL-1β. Mechanistic study revealed that MFA decreased the TAC and the levels of ROS and TBARS. Furthermore, MFA treatment led to a reduction of the mRNA and protein expression of NOX4 and p22phox, as well as the protein levels of caspase3, cleaved caspase-3 and Bax, and an upregulation of p-JNK, p-p38 MAPK and Bcl-2 proteins in the liver. The present study demonstrated that MFA has hepatoprotective effects against CCl4-induced acute liver damage. MFA has anti-oxidant, anti-inflammatory and anti-apoptotic activities and was able to modulate the NOX4/p22phox/ROS-JNK/p38 MAPK signaling pathway.

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Carnosic acid nanoparticles suppress liver ischemia/reperfusion injury by inhibition of ROS, Caspases and NF-κB signaling pathway in mice

Cited by 46 publications

References 36 publications

Apple phlorizin supplementation attenuates oxidative stress in hamsters fed a high-fat diet

Apple phlorizin supplementation attenuates oxidative stress in hamsters fed a high-fat diet

Role of mitofusin 2 in the protective effect of breviscapine against hepatic ischemia/reperfusion injury in rats

Hepatoprotective effect of methyl ferulic acid against carbon tetrachloride-induced acute liver injury in rats

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