Apple phlorizin supplementation attenuates oxidative stress in hamsters fed a high-fat diet

Abstract

Phlorizin from apple peel has been used as a nutrient for more than 100 years. The present study investigated the effects of phlorizin on the antioxidant capacity and the antioxidative mechanisms in hamsters fed high-fat diet supplemented with phlorizin (0.3, 0.6, and 0.9%). Results showed that phlorizin increased the antioxidant enzyme activities and reduced the malondialdehyde content in plasma, liver, heart, and kidney that might arise from the higher mRNA levels of CuZn-superoxide dismutase, Mn-superoxide dismutase (Mn-SOD), catalase, glutathione peroxidase, nuclear factor erythroid 2-related factor 2-like (Nrf2), and the protein expression of Mn-SOD (p < .05). Diet supplemented with phlorizin also significantly decreased the total cholesterol and triglycerides levels in plasma. The present study demonstrated that phlorizin might reduce lipid peroxidation and protect against cardiovascular issues in vivo. Practical applicationsPhlorizin, from apple peel, has been used as a nutrient for over 100 years. To date, despite extensive research on phlorizin, a report on its effect on lipid peroxidation, the antioxidant system in plasma, heart, liver, and kidney of hamsters fed high-fat diet is yet lacking. This report demonstrates that phlorizin can reduce lipid peroxidation and exert a protective effect on cardiovascular issues in vivo, which is valuable in the rational utilization of phlorizin in functional foods. K E Y W O R D Santioxidant capacity, cholesterol, hamsters, phlorizin

Protective effect of apple phlorizin on hydrogen peroxide‐induced cell damage in HepG2 cells

“…These results implied that phlorizin alleviated the oxidative stress induced by H 2 O 2 by upregulating the related activities of antioxidant enzymes. This phenomenon was consistent with that in previous study showed that phlorizin exerted antioxidative effect by regulating the activities of antioxidant enzymes (Wang et al, ).…”

Apple phlorizin attenuates oxidative stress inDrosophila melanogaster

“…The antioxidants work by scavenging the free radicals, thereby inhibiting the activity of ROS. The antioxidants including endogenous antioxidant enzymes such as SOD, CAT, and exogenous dietary antioxidants such as vitamins C and E are essential defense anchors (Wang, Sun, Rehman, Shen et al, 2017). In the present study, the activities of SOD and CAT in fruit flies fed different dosages of phlorizin increased (Figure 2); 2.0 mg/ml phlorizin could significantly increase the CuZnSOD, MnSOD, and CAT enzyme activities.…”

“…MDA is a major oxidative degradation product of membrane unsaturated fatty acids. The level of MDA, which indicates the degree of lipid peroxidation, is an oxidative stress marker (Wang, Sun, Rehman, Wang et al, ). The present study demonstrated that phlorizin treatment could reduce the MDA level in fruit flies in vivo compared with that of the control group (Figure ).…”

Dietary Supplementation of Apple Phlorizin Attenuates the Redox State Related to Gut Microbiota Homeostasis in C57BL/6J Mice Fed with a High-Fat Diet

“…Similarly, our previous study showed that phlorizin supplementation attenuates serum and liver oxidative stress by increasing SOD, CAT, GSH-PX, and T-AOC activity and reduces the MDA content by promoting the gene expression of Nrf2 in hamsters fed with HFDs. 8 However, there is an intimate association between inflammation and redox alteration. 36 Researchers have found that a dysfunction in the Nrf2 system is related to the initiation of inflammation, which, in turn, promotes ROS production, leading to redox imbalance by triggering redox-sensitive transcription factors and signal transduction pathways, further leading to obesity and consequent metabolic aberrances.…”