Cardiac Troponin I (cTnI) concentration in an ovine model of myocardial ischemia

Leonardi, Fabio; Passeri, Benedetta; Fusari, A.; Razza, P. De; Beghi, Cesare; Lorusso, Roberto; Corradi, A.; Botti, Paolo

doi:10.1016/j.rvsc.2007.09.010

Cited by 27 publications

(29 citation statements)

References 21 publications

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“…Yarbrough et al [18] recorded a peak concentration as soon as 90 min after the coronary occlusion in the pig. Leonardi et al [20] demonstrated that cTnI levels began to increase at day 1 to 3 after coronary ligation and after myoblast implantation and gradually [21] and in a dog model [22]. This relation was confirmed in the present study.…”

Section: Discussionsupporting

confidence: 89%

Closed-chest animal model of chronic coronary artery stenosis. Assessment with magnetic resonance imaging

Bogaert

D’hooge

et al. 2009

Int J Cardiovasc Imaging

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To evaluate the consequences of chronic non-occlusive coronary artery (CA) stenosis on myocardial function, perfusion and viability, we developed a closed-chest, closed-pericardium pig model, using magnetic resonance imaging (MRI) as quantitative imaging tool. Pigs underwent a percutaneous copper-coated stent implantation in the left circumflex CA (n = 19) or sham operation (n = 5). To evaluate the occurrence of myocardial infarction, cardiac troponin I (cTnI) levels were repetitively measured. At week 6, CA stenosis severity was quantified with angiography and cine, first-pass and contrast-enhanced MRI were performed to evaluate cardiac function, perfusion and viability. In the stenting group, cTnI values significantly increased at day 3 and day 5 (P = 0.01), and normalized at day 12. At angiography, 13/19 stented pigs had a stenosis >75%. Mean degree of CA stenosis was 91 +/- 4%, range 83-98%. At contrast-enhanced MRI, mean infarct size was 7 +/- 6%, range 0.7-18.4%. Five of the 6 pigs with stenosis <75% had no infarction. Stented pigs showed significantly higher Left-ventricular volumes and normalized mass (P < 0.05), and lower ejection fraction (P = 0.03) than the sham pigs. Both wall thickening and myocardial perfusion were significantly lower in animals with at least one segment >50% infarct (23 +/- 8%; 0.05 +/- 0.01 a.u./s) and animals with only <50% infarct segments (29% +/- 12%; 0.07 +/- 0.02 a.u./s), than sham pigs (52 +/- 6%; 0.10 +/- 0.03 a.u./s) (P < 0.001; P < 0.05). This minimally-invasive animal model of chronic, non-occlusive CA stenosis, presenting a mixture of perfusion and functional impairment and a variable degree of myocardial necrosis, can be used as substitute to study chronic myocardial hypoperfusion.

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Section: Discussionsupporting

confidence: 89%

Closed-chest animal model of chronic coronary artery stenosis. Assessment with magnetic resonance imaging

Bogaert

D’hooge

et al. 2009

Int J Cardiovasc Imaging

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“…cTnI levels ranged from people with myocardial infarction, such lesions are not common in animals. Cardiac troponins were evaluated in some pathologic conditions in ruminants including white muscle disease in lambs, (Gunes et al, 2010), experimental coronary ligation in sheep (Leonardi et al, 2008), foot and mouth disease in calves (Gunes et al, 2005;Tunca et al, 2008), bovine theileriosis (Fartashvand et al, 2013), experimental endotoxemia in calves (Peek et al, 2008). There are some reports about myocardial injury in sheep with acute lactic acidosis (Kirbas et al, 2014) which ranged from changes in ECG (Jafari-Dehkordi et al, 2011;Onmaz et al, 2011) to severe focal myocarditis (Dshurov, 1976).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of serum cardiac biomarkers in sheep with acute lactic acidosis

Fartashvand

Hajisadeghi

2018

J Hellenic Vet Med Soc

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In this study we investigated the changes of cardiac injury biomarkers in serum samples from 200 sheep with acute ruminal lactic acidosis (ARLA) and 50 healthy controls belonging to the Ghezel breed. After clinical examination and recording of vital signs (heart rate, respiratory rate, rectal temperature and hydration status), rumen fluid and venous blood samples were collected. The pH of rumen fluid was determined using a paper tape pH-meter and lactic acid concentration of serum was measured using a commercially available method. Similarly, activities of AST, LDH, CK-MB and serum concentration of cTnI were measured using special commercial kits. According to the findings serum activities of AST (p = 0.007) and CK-MB (p = 0.002) in sheep with ARLA were significantly higher than in healthy animals. Serum LDH activity in the disease group was higher than in the control group, however this difference was statistically non-significant. cTnI concentration were 0.684 ± 0.03 ng/ml in sheep with ARLA, which was significantly higher than in healthy sheep (p = 0.000). There were significant negative correlations between ruminal pH and serum lactate levels, heart rate, respiratory rate and dehydration degree. The sheep with the lowest ruminal pH (3 cases with < 4.5) had the highest levels of cTnI (2.28 to 3.06 ng/mL), and all died. It can be concluded that lactic acidosis may cause some degree of heart damage, although further studies are needed to support this speculation.

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“…Cardiac troponin assays designed for human use are a sensitive and specifi c marker for the determination of myocardial degeneration and necrosis in animals [15,22]. Some biomarkers, such as CK-MB and LDH, do not come close to the cardiac troponin biomarker in the evaluation of myocardial injury.…”

Section: Discussionmentioning

confidence: 99%

“…Cardiac troponin I concentrations have been reported in various diseases in cattle [14][15][16] but not in BRD. Therefore, the present study was aimed to evaluate the serum cardiac troponin I concentrations in weaned calves with moderate and severe BRD.…”

Section: Introductionmentioning

confidence: 97%

Cardiac Troponin-I Concentration In Weaned Calves With Bovine Respiratory Disease

et al. 2015

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The aim of this study was to evaluate the serum cardiac troponin I (cTnI) concentrations in weaned calves with moderate and severe bovine respiratory disease. Eighteen healthy weaned calves (group I), 18 weaned calves with moderate bovine respiratory disease (group II) and 18 weaned calves with severe bovine respiratory disease (group III) were included in the study. Clinical signs and severity of respiratory disease according to clinical index scores were determined. Blood cell counts and cardiac biomarkers, such as serum cTnI concentrations and creatine kinase-myocardial band (CK-MB), and lactate dehydrogenase (LDH) activities were determined in all groups. Temperature and respiratory and pulse rates were signifi cantly increased in calves with moderate and severe bovine respiratory disease compared to healthy calves (P < 0.05). Serum cTnI concentrations and LDH activities were signifi cantly increased in the calves with severe bovine respiratory disease compared to healthy calves and calves with moderate bovine respiratory disease (P < 0.05). A signifi cant increase in white blood cell counts in calves with moderate and severe bovine respiratory disease and lymphopenia and neutrophilia in calves with severe bovine respiratory disease were also found. The study suggests that severe bovine respiratory disease causes increased serum cTnI concentrations in weaned calves.

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Cardiac Troponin I (cTnI) concentration in an ovine model of myocardial ischemia

Cited by 27 publications

References 21 publications

Closed-chest animal model of chronic coronary artery stenosis. Assessment with magnetic resonance imaging

Closed-chest animal model of chronic coronary artery stenosis. Assessment with magnetic resonance imaging

Evaluation of serum cardiac biomarkers in sheep with acute lactic acidosis

Cardiac Troponin-I Concentration In Weaned Calves With Bovine Respiratory Disease

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