Carbosilane dendrimers with phosphonium terminal groups are low toxic non-viral transfection vectors for siRNA cell delivery

Herma, Regina; Wrobel, Dominika; Liegertová, Michaela; Müllerová, Monika; Strašák, Tomáš; Malý, Marek; Semerádtová, Alena; Štofik, Marcel; Appelhans, Dietmar; Malý, Jan

doi:10.1016/j.ijpharm.2019.03.018

Cited by 17 publications

(19 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the size of nanoparticles and their surface charge were previously identified as important factors influencing both the cellular uptake [58] and cytotoxicity effects [29,31,[59][60][61][62][63][64], the behavior of prepared amphiphilic dendrons in water was investigated in terms of hydrodynamic size and Zeta potential. These parameters are crucial in the case of nanoparticles intended for biomedical applications.…”

Section: Dynamic Light Scattering and Zeta Potentialmentioning

confidence: 99%

“…The cycle was repeated until sufficient purity was reached. Removal of solvents was performed on a rotary evaporator at maximum 50 29 Si spectra were referenced to external standard hexamethyldisilane (δ/ppm; −19.87 ppm). The 19 F NMR spectra were referenced to external standard hexafluorobenzene (δ/ppm; −163.86).…”

Section: General Remarksmentioning

confidence: 99%

“…Our long-term interest has been focused on CS dendrimers with a well-defined structure that express multivalent presentation of functional moieties at their periphery [27][28][29][30][31] and, due to the incompatibility of their hydrophobic interior with polar periphery, can be considered as unimolecular micelles [32]. Here we turn our attention to a development of new versatile synthetic protocols for amphiphilic building blocks capable of self-assembly to regular micelles and other supramolecular objects.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adaptive Synthesis of Functional Amphiphilic Dendrons as a Novel Approach to Artificial Supramolecular Objects

Edr

Wrobel

Krupková

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Supramolecular structures, such as micelles, liposomes, polymerosomes or dendrimerosomes, are widely studied and used as drug delivery systems. The behavior of amphiphilic building blocks strongly depends on their spatial distribution and shape of polar and nonpolar component. This report is focused on the development of new versatile synthetic protocols for amphiphilic carbosilane dendrons (amp-CS-DDNs) capable of self-assembly to regular micelles and other supramolecular objects. The presented strategy enables the fine modification of amphiphilic structure in several ways and also enables the facile connection of a desired functionality. DLS experiments demonstrated correlations between structural parameters of amp-CS-DDNs and the size of formed nanoparticles. For detailed information about the organization and spatial distribution of amp-CS-DDNs assemblies, computer simulation models were studied by using molecular dynamics in explicit water.

show abstract

Section: Dynamic Light Scattering and Zeta Potentialmentioning

confidence: 99%

Section: General Remarksmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adaptive Synthesis of Functional Amphiphilic Dendrons as a Novel Approach to Artificial Supramolecular Objects

Edr

Wrobel

Krupková

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The treatment methods herein all have a common feature that requires carrier cooperation. Although viruses can be used as carriers, viral vectors have been confirmed to cause insertional mutagenesis and immunogenicity [ 2 ]. Therefore, finding a safer and more effective carrier has become a top priority.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles: A New Approach to Upgrade Cancer Diagnosis and Treatment

Gao

Chen

et al. 2021

Nanoscale Res Lett

View full text Add to dashboard Cite

Traditional cancer therapeutics have been criticized due to various adverse effects and insufficient damage to targeted tumors. The breakthrough of nanoparticles provides a novel approach for upgrading traditional treatments and diagnosis. Actually, nanoparticles can not only solve the shortcomings of traditional cancer diagnosis and treatment, but also create brand-new perspectives and cutting-edge devices for tumor diagnosis and treatment. However, most of the research about nanoparticles stays in vivo and in vitro stage, and only few clinical researches about nanoparticles have been reported. In this review, we first summarize the current applications of nanoparticles in cancer diagnosis and treatment. After that, we propose the challenges that hinder the clinical applications of NPs and provide feasible solutions in combination with the updated literature in the last two years. At the end, we will provide our opinions on the future developments of NPs in tumor diagnosis and treatment.

show abstract

“…Gene silencing by short interfering RNA (siRNA) provides a promising prospect for cervical cancer therapy (Li et al, 2019a). Although viruses have been used as a gene delivery vehicle, the viral vehicle is prone to the risk of insertional mutagenesis and immunogenicity (Herma et al, 2019). Thus, nonviral vehicles hold enormous potential for cancer gene therapy because of their superior safety (Kim et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Functionalized selenium nanoparticles for targeted siRNA delivery silence Derlin1 and promote antitumor efficacy against cervical cancer

et al. 2019

View full text Add to dashboard Cite

2020) Functionalized selenium nanoparticles for targeted siRNA delivery silence Derlin1 and promote antitumor efficacy against cervical cancer, Drug Delivery, 27:1, 15-25, ABSTRACT Small interfering RNA (siRNA) exhibits great potential as a novel therapeutic option due to its highly sequence-specific ability to silence genes. However, efficient and safe delivery carriers are required for developing novel therapeutic paradigms. Thus, the successful development of efficient delivery platforms for siRNA is a crucial issue for the development of siRNA-based drugs in cancer treatments. In this study, biocompatible selenium nanoparticles (SeNPs) were loaded with RGDfC peptide to fabricate tumor-targeting gene delivery vehicle RGDfC-SeNPs. Subsequently, RGDfC-SeNPs were loaded with Derlin1-siRNA to fabricate RGDfC-Se@siRNA, which are functionalized selenium nanoparticles. RGDfC-Se@siRNA showed greater uptake in HeLa cervical cancer cells in comparison with that in human umbilical vein endothelial cells (HUVECs), verifying the RGDfC-mediated specific uptake of RGDfC-Se@siRNA. RGDfC-Se@siRNA was capable of entering HeLa cells via clathrin-associated endocytosis, and showed faster siRNA release in a cancer cell microenvironment in comparison with a normal physiological environment. qPCR and western blotting assays both indicated that RGDfC-Se@siRNA exhibited an obvious gene silencing efficacy in HeLa cells. RGDfC-Se@siRNA suppressed the invasion, migration and the proliferation of HeLa cells, and triggered HeLa cell apoptosis. Moreover, RGDfC-Se@siRNA induced the disruption of mitochondrial membrane potentials. Meanwhile, RGDfC-Se@siRNA enhanced the generation of reactive oxygen species (ROS) in HeLa cell, suggesting that mitochondrial dysfunction mediated by ROS might play a significant role in RGDfC-Se@siRNA-induced apoptosis. Interestingly, RGDfC-SeNPs@siRNA exhibited significant antitumor activity in a HeLa tumor-bearing mouse model. Additionally, RGDfC-SeNPs@siRNA is nontoxic to main organ of mouse. The above results indicate that RGDfC-Se@siRNA provides a promising potential for cervical cancer therapy. ARTICLE HISTORY

show abstract

Carbosilane dendrimers with phosphonium terminal groups are low toxic non-viral transfection vectors for siRNA cell delivery

Cited by 17 publications

References 54 publications

Adaptive Synthesis of Functional Amphiphilic Dendrons as a Novel Approach to Artificial Supramolecular Objects

Adaptive Synthesis of Functional Amphiphilic Dendrons as a Novel Approach to Artificial Supramolecular Objects

Nanoparticles: A New Approach to Upgrade Cancer Diagnosis and Treatment

Functionalized selenium nanoparticles for targeted siRNA delivery silence Derlin1 and promote antitumor efficacy against cervical cancer

Contact Info

Product

Resources

About