Carbon nanotubes-loaded subunit vaccine can increase protective immunity against rhabdovirus infections of largemouth bass (Micropterus Salmoides)

Guo, Zi‐Rao; Zhao, Zhao; Zhang, Chen; Jia, Yijun; Qiu, De-Kui; Zhu, Bin; Wang, Gao‐Xue

doi:10.1016/j.fsi.2020.02.055

Cited by 42 publications

(29 citation statements)

References 32 publications

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“…SWCNTs comprise a single graphene cylinder, while MWCNTs comprise multiple coaxial cylinders [159]. Recently, SWCNTs have been extensively studied as carriers to harbor subunit components for the development of fish vaccines [160][161][162][163][164]. Jia et al conjugated a recombinant protein antigen to oxidized SWCNTs (1-2 nm in outside diameter and 0.4-3 µm in length) via carbodiimide chemistry [164].…”

Section: Protein-inorganic Substance Nanoconjugatesmentioning

confidence: 99%

Chemical Conjugation Strategies for the Development of Protein-Based Subunit Nanovaccines

Duong

Shalash

et al. 2021

Vaccines

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The production of subunit nanovaccines relies heavily on the development of a vaccine delivery system that is safe and efficient at delivering antigens to the target site. Nanoparticles have been extensively investigated for vaccine delivery over the years, as they often possess self-adjuvanting properties. The conjugation of antigens to nanoparticles by covalent bonds ensures co-delivery of these components to the same subset of immune cells in order to trigger the desired immune responses. Herein, we review covalent conjugation strategies for grafting protein or peptide antigens onto other molecules or nanoparticles to obtain subunit nanovaccines. We also discuss the advantages of chemical conjugation in developing these vaccines.

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Section: Protein-inorganic Substance Nanoconjugatesmentioning

confidence: 99%

Chemical Conjugation Strategies for the Development of Protein-Based Subunit Nanovaccines

Duong

Shalash

et al. 2021

Vaccines

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“…The filtered mixture was washed three times with PBS (pH 7.4) to obtain mannosylated G protein (MG). Then, according to our previous research, MG and functionalized single-walled carbon nanotubes (SWCNTs) are coupled to form SWCNTs-MG (Guo et al, 2020;Jia, Guo, et al, 2020;Zhang et al, 2020). The well-mixed SWCNTs-MG was placed in a freeze dryer and lyophilized into powder, and stored at 4℃ for later use.…”

Section: Preparation Of Swcnts-mg Vaccinementioning

confidence: 99%

Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

Gong

Zhang

et al. 2021

Journal of Fish Diseases

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Immersion vaccination of single-walled carbon nanotubes loaded with mannosemodified glycoprotein (SWCNTs-MG) vaccine has been proved to be effective in preventing spring viraemia of carp virus (SVCV). Immunization procedure has immense consequence on the immune effect of the immersion vaccine. However, immunization procedure optimization for SWCNTs-MG vaccine against SVCV has not been reported. In this study, accordingly, a full-factor experiment was designed to optimize the immunization procedure of SWCNTs-MG vaccine by three aspects of vaccine dose (30 mg/L, 40 mg/L and 50 mg/L), immunization density (8 fish L −1 , 24 fish L −1 and 48 fish L −1 ) and immunization time (6, 12 and 24 hr). Furthermore, we used the immunization group (A1B2C1, 30 mg/L, 24 fish L −1 and 6 hr) in the previous study as a positive control (PC) to evaluate the immunization effect optimized conditions from the expression of immune-related genes and relative percentage survival (RPS). At 28 days post-vaccination (DPV), common carps were intraperitoneal injected SVCV challenged test indicated that the A1B2C2 group (30 mg/L, 24 fish L −1 , 12 hr) displayed superiority of protective efficacy compare with other groups and the RPS with 77.9%, which was 15.6% higher than the PC group of RPS with 62.3%.Moreover, the expression of immune-related genes such as IL-10, CD4 and MHC-II was also significantly higher than PC group. The specific experimental flow chart is shown in Figure 1. Conclusively, these results demonstrated that vaccine dose, immunization density and immunization time are 30 mg/L, 24 fish L −1 and 12 hr, which is the more appropriate immunization programme with juvenile carp for SWCNTs-MG vaccine. This study provides a profitable reference for improving the immune efficiency of aquatic immersion vaccine.

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“…Although several research teams have conducted vaccine researches and developments to protect against MSRV (Guo et al., 2020; Zhang et al., 2018), there are currently no available and effective prevention or therapy options for this disease. Moreover, apart from the body surface and fins with punctate haemorrhages, fish infected with MSRV show no distinctive diagnostic clinical features of the disease (Fu et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, it is necessary to detect the virus rapidly and prevent further spread of this disease hopefully. For diagnosis of MSRV, researchers have used polymerase chain reaction (PCR) (Fu et al., 2017), quantitative real‐time polymerase chain reaction (qPCR) (Zhang et al., 2018), enzyme‐linked immunosorbent assay (ELISA) (Guo et al., 2020), histopathology (Lei et al., 2015) and virus isolation (Ma et al., 2013), but a more efficient and convenient diagnostic method is still needed.…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Rapid visual detection of Micropterus salmoides rhabdovirus using recombinase polymerase amplification combined with lateral flow dipsticks

Feng

Chu

Han

et al. 2022

Journal of Fish Diseases

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Largemouth bass (Micropterus salmoides), a member of the family Centrarchidae, is a freshwater fish species originating from North America, introduced into Guangdong Province, China, in 1983(Guo et al., 2020. Nowadays, owing to its breeding advantages such as high adaptability, rapid growth and delicious flesh, largemouth bass becomes one of the most popular and important aquaculture species with extensive cultivation and breeding (Huang et al., 2021), and its annual production has already reached 477,808 tons (Ministry of Agriculture & Rural Affairs Fisheries Administration Bureau, 2020). Unfortunately, a new viral disease impacted the largemouth bass aquaculture industry in Guangdong Province, China, in 2011, resulting in 200,000 fish deaths and substantial economic losses (Ma et al., 2013). Subsequently, rhabdovirus was confirmed as the pathogen and several rhabdovirus isolates were reported (

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Carbon nanotubes-loaded subunit vaccine can increase protective immunity against rhabdovirus infections of largemouth bass (Micropterus Salmoides)

Cited by 42 publications

References 32 publications

Chemical Conjugation Strategies for the Development of Protein-Based Subunit Nanovaccines

Chemical Conjugation Strategies for the Development of Protein-Based Subunit Nanovaccines

Optimization of immunization procedure for SWCNTs‐based subunit vaccine with mannose modification against spring viraemia of carp virus in common carp

Rapid visual detection of Micropterus salmoides rhabdovirus using recombinase polymerase amplification combined with lateral flow dipsticks

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