Carbon monoxide releasing molecule-2 attenuates Pseudomonas aeruginosa-induced ROS-dependent ICAM-1 expression in human pulmonary alveolar epithelial cells

Lee, Chiang‐Wen; Wu, Cheng‐Hsun; Chiang, Yu-Ting; Chen, Yuh‐Lien; Chang, Kuo-Ting; Chuang, Chu-Chun; Lee, I-Te

doi:10.1016/j.redox.2018.07.001

Cited by 21 publications

(12 citation statements)

References 41 publications

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“…Pseudomonas , a gram-negative bacterium with carbon degradation activity, was the most common pathogen for hospital infection (Hota et al, 2009; Decker and Palmore, 2014; Hung et al, 2016). It can invade the epithelium and induce immune responses through activation of protein kinase C alpha (PKCα), c-Jun N -terminal kinase (JNK), extracellular-regulated protein kinases (ERK1/2), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and/or glutamic acid–leucine–arginine–positive CXC chemokines (Gregson et al, 2013; Chiang-Wen et al, 2018; Curran et al, 2018). Aggregatibacter was associated with aggressive periodontitis (Ando et al, 2010), which can induce inflammatory response through cytolethal distending toxin (CDT), leukotoxin, and lipopolysaccharide (LPS) (Herbert et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Variations in Oral Microbiota Composition Are Associated With a Risk of Throat Cancer

Wang¹,

Yin

Guo³

et al. 2019

Front. Cell. Infect. Microbiol.

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In this study, a next-generation sequencing strategy on 16 S ribosomal RNA (16 S rRNA) gene was employed to analyze 70 oral samples from 32 patients with throat cancer, nine patients with vocal cord polyp, and 29 healthy individuals (normal controls). Using this strategy, we demonstrated, for the first time, that the salivary microbiota of cancer patients were significantly different from those of patients with a polyp and healthy individuals. We observed that the beta diversity of the cancer group was divergent from both the normal and polyp groups, while alpha-diversity indices such as the Chao1 estimator ( P = 8.1e-05), Simpson ( P = 0.0045), and Shannon ( P = 0.0071) were significantly reduced in cancer patients compared with patients containing a polyp and normal healthy individuals. Linear discriminant analysis (LDA) and Kruskal–Wallis test analyses and real-time quantitative polymerase chain reaction (qPCR) verification test revealed that the genera Aggregatibacter, Pseudomonas, Bacteroides , and Ruminiclostridium were significantly enriched in the throat cancer group compared with the vocal cord polyp and normal control groups (score value >2). Finally, diagnostic models based on putatively important constituent bacteria were constructed with 87.5% accuracy [area under the curve (AUC) = 0.875, 95% confidence interval (CI): 0.695–1]. In summary, in this study we characterized, for the first time, the oral microbiota of throat cancer patients without smoking history. We speculate that these results will help in the pathogenic mechanism and early diagnosis of throat cancer.

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Section: Discussionmentioning

confidence: 99%

Variations in Oral Microbiota Composition Are Associated With a Risk of Throat Cancer

Wang¹,

Yin

Guo³

et al. 2019

Front. Cell. Infect. Microbiol.

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“… 36 Moreover, P. aeruginosa infection can activate all three MAPK members, further leading to apoptosis through ROS accumulation. 37 In addition, many lines of evidence confirmed that rapamycin can alter the activity of MAPKs, which further affects cell proliferation, survival and apoptosis. 38 – 40 We proposed that the autophagy-mediated regulation of MAPK signalling pathway and scavenging ROS should be critical for inhibiting P. aeruginosa- induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Autophagy plays a protective role duringPseudomonas aeruginosa-induced apoptosis via ROS–MAPK pathway

Han

et al. 2020

Innate Immun

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Pseudomonas aeruginosa infection can induce alveolar macrophage apoptosis and autophagy, which play a vital role in eliminating pathogens. These two processes are usually not independent. Recently, autophagy has been found to interact with apoptosis during pathogen infections. Nevertheless, the role of autophagy in P. aeruginosa-infected cell apoptosis is unclear. In this study, we explored the impact of P. aeruginosa infection on autophagy and apoptosis in RAW264.7 cells. The autophagy activator rapamycin was used to stimulate autophagy and explore the role of autophagy on apoptosis in P. aeruginosa-infected RAW264.7 cells. The results indicated that P. aeruginosa infection induced autophagy and apoptosis in RAW264.7 cells, and that rapamycin could suppress P. aeruginosa-induced apoptosis by regulating the expression of apoptosis-related proteins. In addition, rapamycin scavenged the cellular reactive oxygen species (ROS) and diminished p-JNK, p-ERK1/2 and p-p38 expression of MAPK pathways in RAW264.7 cells infected with P. aeruginosa. In conclusion, the promotion of autophagy decreased P. aeruginosa-induced ROS accumulation and further attenuated the apoptosis of RAW264.7 cells through MAPK pathway. These results provide novel insights into host–pathogen interactions and highlight a potential role of autophagy in eliminating P. aeruginosa.

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“…In the TNF-a and IL-1b exposed human gingival fibroblasts, CORM-3 inhibited adhesion molecules expression by suppressing the NF-kB pathway (Song et al, 2011). Additionally, Cunha and his colleagues demonstrated that the CO donor, dimanganese decacarbonyl, sustained the neutrophils adhesion and migration and ICAM-1 expression depending on soluble guanylate cyclase (sGC) activation (Lee et al, 2018). By the isolating the neutrophils from the PKG-deficient mice, they found the HO/CO/PKG pathway involves in neutrophil migration which might be further explain by the PKG effect on the NF-kB phosphorylation.…”

Section: Discussionmentioning

confidence: 99%

CO-Releasing Molecule (CORM)-3 Ameliorates Spinal Cord-Blood Barrier Disruption Following Injury to the Spinal Cord

Zheng

Luo

et al. 2020

Front. Pharmacol.

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Spinal cord injury (SCI) is a clinical tough neurological problem without efficient cure currently. Blood-spinal cord barrier (BSCB) interruption is not only a crucial pathological feature for SCI process but is a possible target for future SCI treatments; however, few treatments have been developed to intervene BSCB. In the present study, we intravenously injected CO-releasing molecule3 (CORM-3), a classical exogenous CO donor, to the rats experiencing SCI and assessed its protection on BSCB integrity in rats. Our results demonstrated that the exogenous increasing of CO by CORM-3 blocked the tight junction (TJ) protein degeneration and neutrophils infiltration, subsequently suppressed the BSCB damage and improved the motor recovery after SCI. And we certified that the CO-induced down-regulation of MMP-9 expression and activity in neutrophil might be associated with the NF-kB signaling. Taken together, our study indicates that CO-releasing molecule (CORM)-3 ameliorates BSCB after spinal cord injury.

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Carbon monoxide releasing molecule-2 attenuates Pseudomonas aeruginosa-induced ROS-dependent ICAM-1 expression in human pulmonary alveolar epithelial cells

Cited by 21 publications

References 41 publications

Variations in Oral Microbiota Composition Are Associated With a Risk of Throat Cancer

Variations in Oral Microbiota Composition Are Associated With a Risk of Throat Cancer

Autophagy plays a protective role duringPseudomonas aeruginosa-induced apoptosis via ROS–MAPK pathway

CO-Releasing Molecule (CORM)-3 Ameliorates Spinal Cord-Blood Barrier Disruption Following Injury to the Spinal Cord

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