Carbon dioxide effects on olfactory functioning: Behavioral, histological and immunohistochemical measurements

Buron, G.; Hacquemand, Romain; Pourié, Grégory; Brand, Gérard

doi:10.1016/j.toxlet.2009.04.018

Cited by 8 publications

(10 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings are in agreement with other previous published studies showing no modification in the urine sensitivity during CO 2 (Buron et al 2009a) or acetone (Buron et al 2009b) exposures. Moreover, in these published experiments as well as in similar studies (Jacquot et al 2006;Buron et al 2007), repeated behavioral measures without specific inhalation exposure in control groups of mice showed no time effect in response to urine.…”

Section: Discussionsupporting

confidence: 94%

“…As previously observed in the main olfactory epithelium during CO 2 exposure (Buron et al 2009a) as well as during acetone exposure (Buron et al 2009b), the phenotype was important regarding the cellular impact. Thus, further research could use specific markers in order to determine what cellular types were concerned by the increasing number in the VNO.…”

Section: Discussionsupporting

confidence: 62%

“…The comparative findings of 3% CO 2 effects between the main olfactory epithelium (Buron et al 2009a) and the vomeronasal epithelium in mice suggest a lower impact on the VNO. First, the changes of thickness appeared more marked in the main olfactory epithelium and continued during the postexposure period, while the epithelium thickness of the VNO recovered the normal structure in the postexposure period.…”

Section: Discussionmentioning

confidence: 87%

“…As the VNO is an encapsulated organ in the nasal cavity, it is hypothesized that the changes could be less pronounced comparatively to those described in the main olfactory epithelium (Buron et al 2009a), which is directly in contact with the inhaled air. Additionally, the pheromonal sensitivity was evaluated in order to test the functional impact of CO 2 exposure.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of CO2 inhalation exposure on mice vomeronasal epithelium

et al. 2009

View full text Add to dashboard Cite

Nasal epitheliums are the first sites of the respiratory tract in contact with the external environment and may therefore be susceptible to damage from exposure to many toxic volatile substances (i.e., volatile organic components, vapors, and gases). In the field of inhalation toxicology, a number of studies have considered the main olfactory epithelium, but few have dealt with the epithelium of the vomeronasal organ (VNO). However, in several species such as in rodents, the VNO (an organ of pheromone detection) plays an important role in social interactions, and alterations of this organ are known to induce adaptative behavioral disturbances. Among volatile toxicants, health effects of inhaled gases have been thoroughly investigated, especially during CO(2) inhalation because of its increasing atmospheric concentration. Therefore, this work was designed to examine the effects of 3% CO(2) inhalation on VNO in two different exposure conditions (5 h/day and 12 h/day) in mice. Behavioral sensitivity tests to urine of congener and histological measurements of VNO were conducted before, during (weeks 1-4), and after (weeks 5-8) CO(2) inhalation exposures. Results showed no significant modifications of behavioral responses to urine, but there were significant changes of both cell number and thickness of the VNO epithelium. Moreover, the findings indicated a selectively dose-dependent effect of CO(2), and further research could use other gases in the same manner for comparison.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of CO2 inhalation exposure on mice vomeronasal epithelium

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Another approach has been the inhalation of volatile chemicals, such as CO 2 [18] and methyl bromide [19,20]. All of these methods rely on a widespread variable degeneration of the ON, destroying multiple cell populations, without specifically targeting olfactory stem cells.…”

Section: Introductionmentioning

confidence: 99%

Morphological and behavioural changes occur following the X-ray irradiation of the adult mouse olfactory neuroepithelium

et al. 2012

View full text Add to dashboard Cite

BackgroundThe olfactory neuroepithelium lines the upper nasal cavity and is in direct contact with the external environment and the olfactory bulbs. The ability to self-renew throughout life and the reproducible recovery after injury, make it a model tissue to study mechanisms underlying neurogenesis. In this study, X-rays were used to disrupt proliferating olfactory stem cell populations and to assess their role in the cellular and morphological changes involved in olfactory neurogenic processes.ResultsWe have analysed the histological and functional effects of a sub-lethal dose of X-rays on the adult mouse olfactory neuroepithelium at 2 hours, 24 hours, 1 week, 2 weeks and 5 weeks. We have shown an immediate cessation of proliferating olfactory stem cells as shown by BrdU, Ki67 and pH3 expression. At 24 hours there was an increase in the neural transcription factors Mash1 and Pax6 expression, and a disruption of the basal lamina and increase in glandular cell marker expression at 1 week post-irradiation. Coincident with these changes was an impairment of the olfactory function in vivo.ConclusionsWe have shown significant changes in basal cell proliferation as well as morphological changes in the olfactory neuroepithelium following X-ray irradiation. There is involvement of the basal lamina as well as a clear role for glandular and sustentacular cells.

show abstract