Carbon Catabolite Repression in Filamentous Fungi

Adnan, Muhammad; Zheng, Wenhui; Islam, Waqar; Arif, Muhammad; Abubakar, Yakubu Saddeeq; Wang, Zonghua; Lu, Guodong

doi:10.3390/ijms19010048

Cited by 164 publications

(153 citation statements)

References 211 publications

(303 reference statements)

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“…Transcription factor CreA and catabolic pathways involved in less favorable carbon sources of P. radiata were not induced under hypoxia. Therefore, we concluded that concentration of glucose or other carbohydrates was not high enough to induce carbon catabolite repression [51] under hypoxia. Expression of the diauxic shift-associated transcription factor Cat8 candidate in P. radiata [37] followed a similar pattern to CreA.…”

Section: Adaptation To Hypoxia By Regulation Of Intracellular Metabolismmentioning

confidence: 89%

Hypoxia is regulating enzymatic wood decomposition and intracellular carbohydrate metabolism in filamentous white rot fungus

Mattila

Mäkinen

Lundell

2020

Biotechnol Biofuels

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Background: Fungal decomposition of wood is considered as a strictly aerobic process. However, recent findings on wood-decaying fungi to produce ethanol from various lignocelluloses under oxygen-depleted conditions lead us to question this. We designed gene expression study of the white rot fungus Phlebia radiata (isolate FBCC0043) by adopting comparative transcriptomics and functional genomics on solid lignocellulose substrates under varying cultivation atmospheric conditions. Results: Switch to fermentative conditions was a major regulator for intracellular metabolism and extracellular enzymatic degradation of wood polysaccharides. Changes in the expression profiles of CAZy (carbohydrate-active enzyme) encoding genes upon oxygen depletion, lead into an alternative wood decomposition strategy. Surprisingly, we noticed higher cellulolytic activity under fermentative conditions in comparison to aerobic cultivation. In addition, our results manifest how oxygen depletion affects over 200 genes of fungal primary metabolism including several transcription factors. We present new functions for acetate generating phosphoketolase pathway and its potential regulator, Adr1 transcription factor, in carbon catabolism under oxygen depletion. Conclusions: Physiologically resilient wood-decomposing Basidiomycota species P. radiata is capable of thriving under respirative and fermentative conditions utilizing only untreated lignocellulose as carbon source. Hypoxiaresponse mechanism in the fungus is, however, divergent from the regulation described for Ascomycota fermenting yeasts or animal-pathogenic species of Basidiomycota.

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Section: Adaptation To Hypoxia By Regulation Of Intracellular Metabolismmentioning

confidence: 89%

Hypoxia is regulating enzymatic wood decomposition and intracellular carbohydrate metabolism in filamentous white rot fungus

Mattila

Mäkinen

Lundell

2020

Biotechnol Biofuels

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“…It is important to note that cellulases and most of the plant cell-wall hydrolyzing enzymes are inducible and are also influenced by catabolite repression in many fungi (Adnan et al, 2017). The decline of endoglucanase activity after the maximum peak of enzyme secretion could be attributed to cumulative release of cellobiose at the start of the hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

Effect of agro-industrial residues mixtures on the production of endoglucanase by Aspergillus niger in solid state fermentation

2019

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The low-cost production of cellulolytic complexes that present high action at mild conditions is one of the major bottlenecks for the economic viability of the production of cellulosic ethanol. The influence of agro-industrial residues was assessed to enhance endoglucanase production by Aspergillus niger 426 grown in solid state fermentation. The highest percentage of lignin degradation was found on soybean hulls (56%) followed by sugarcane bagasse (36%) and rice straw (8.5%). The cellulose degradation, around 90%, was observed on soybean hulls and sugarcane bagasse, but only 50% on rice straw, and maximum production of endoglucanase (112.34 ± 0.984 U mL-1) was observed for soybean hulls. The best Experimental Mixture Design condition was under cultivation of 2.5 g of sugarcane bagasse, 2.3 g of rice straw and 5.2 g of soybean hulls, leading to a maximum activity of 138.92 ± 0.02 U mL-1. The statistical methodology enabled an increase of over 20% in the production of endoglucanase using agro-industrial waste. These data demonstrate that A. niger 426 is a potential source of cellulases which can be obtained by solid state fermentation using agro-industrial waste.

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“…The same repressor has been proved to influence the synthesis of lignolytic enzymes (Janusz et al, 2013), cellulases (Bischof et al, 2016), and hemicellulases (Amore et al, 2013). Moreover, it may also affect fungal growth, virulence, morphology, and utilisation of nonglucose sources (Adnan et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Light-regulated synthesis of extra- and intracellular enzymes related to wood degradation by the white rot fungus Cerrena unicolor during solid-state fermentation on ash sawdust-based medium

et al. 2019

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The light-dependent metabolism of the white rot basidiomycete Cerrena unicolor FCL139 has already been demonstrated using transcriptomic and Biolog-based approaches. To further analyze the influence of light on C. unicolor wood degradation, we measured the activity of an array of CAZymes (carbohydrate-active enzymes) and enzymes involved in the redox system of fungal cells associated with lignolysis. Extra- and intracellular enzymatic extracts were obtained from solid-state ash sawdust C. unicolor cultures cultivated for 14 days under red, blue, green, or white light conditions, or in the dark. Light greatly influenced the synthesis of MnP, total cellulases, endo-1,4-β-glucanase, endo-1,4-β-xylanase, catalase, and superoxide dismutase. The production of MnP and catalase was evidently stimulated by white light. It is also worth noticing that blue light caused a gradual increase in the activity of total cellulases throughout the entire period of C. unicolor growth. Moreover, endo-1,4-β-glucanase showed the highest activity on day 13 of fungus cultivation and the production of laccase and β-glucosidase appeared to be the least influenced by light. However, the strongest activity of the endo-1,4-β-xylanase was observed in the dark. It seemed that light not only influenced the regulation of the synthesis of the wood-degrading enzymes at different levels, but also acted indirectly by affecting production of enzymes managing harmful lignin by-products causing oxidative stress. The ability of the fungus to decompose woody plant material is clearly influenced by environmental factors.

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Carbon Catabolite Repression in Filamentous Fungi

Cited by 164 publications

References 211 publications

Hypoxia is regulating enzymatic wood decomposition and intracellular carbohydrate metabolism in filamentous white rot fungus

Hypoxia is regulating enzymatic wood decomposition and intracellular carbohydrate metabolism in filamentous white rot fungus

Effect of agro-industrial residues mixtures on the production of endoglucanase by Aspergillus niger in solid state fermentation

Light-regulated synthesis of extra- and intracellular enzymes related to wood degradation by the white rot fungus Cerrena unicolor during solid-state fermentation on ash sawdust-based medium

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