Carbohydrate transporting membrane proteins of the rumen bacterium, Butyrivibrio proteoclasticus

Bond, J. J.; Dunne, Jonathan C.; Kwan, Fiona Y.-S.; Liu, Dong; Zhang, Kunkun; Leahy, Sinead C.; Kelly, William J.; Attwood, Graeme T.; Jordan, T. William

doi:10.1016/j.jprot.2011.12.013

Cited by 17 publications

(24 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B. proteoclasticus cells were grown at 37 °C under anaerobic conditions in modified DSM Medium 704 that included 0.2% yeast extract and 0.2% trypticase peptone, with either 0.1% ( w / v ) oat-spelt xylan or 0.5% ( w / v ) xylose [10,13]. Mid-log phase and stationary phase cultures were harvested at approximately OD 600 0.5 and OD 600 0.7, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Extracted tryptic peptides were resuspended in 1.5 µL of 10 mg.mL −1 α-cyano-4-hydroxycinnamic acid in 50% acetonitrile: 0.1% trifluoroacetic acid ( v / v ) and analyzed using a Voyager DE Pro MALDI-TOF mass spectrometer (Applied Biosystems, Foster City, CA, USA) as previously described [10,13,16]. MALDI MS/MS using an ABSCIEX 5800 TOF/TOF mass spectrometer (SCIEX, Framingham, MA, USA) was used for repeat analysis of protein spots that contained fibrolytic enzymes.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, the secreted xylosidase/arabinofuranosidase Xsa43J is expected to hydrolyze xylobiose, arabinoxylans and arabinogalactans. Several ATP-binding cassette (ABC) transporter substrate-binding proteins were also found to be significantly more abundant in the xylan-grown cell culture medium [10], and in the membrane proteome [13], which implies they are important for uptake of oligosaccharides derived from xylan disassembly.…”

Section: Introductionmentioning

confidence: 99%

“…Two previously described proteomic workflows [10,13] were used: (1) separation of proteins by 2DE followed by use of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF and TOF/TOF) mass spectrometry for identification of protein spots excised from the gels; and (2) liquid chromatography–tandem mass spectrometry (LC-MS/MS) of tryptic digests of the cytosolic samples.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Cytosolic Oligosaccharide-Degrading Proteome of Butyrivibrio Proteoclasticus

et al. 2015

Self Cite

View full text Add to dashboard Cite

The growth and productivity of ruminants depends on a complex microbial community found in their fore-stomach (rumen), which is able to breakdown plant polysaccharides and ferment the released sugars. Butyrivibrio proteoclasticus B316T is a Gram-positive polysaccharide-degrading, butyrate-producing bacterium that is present at high numbers in the rumen of animals consuming pasture or grass silage based diets. B316T is one of a small number of rumen fibrolytic microbes capable of efficiently degrading and utilizing xylan, as well as being capable of utilizing arabinose, xylose, pectin and starch. We have therefore carried out a proteomic analysis of B316T to identify intracellular enzymes that are implicated in the metabolism of internalized xylan. Three hundred and ninety four proteins were identified including enzymes that have potential to metabolize assimilated products of extracellular xylan digestion. Identified enzymes included arabinosidases, esterases, an endoxylanase, and β-xylosidase. The presence of intracellular debranching enzymes indicated that some hemicellulosic side-chains may not be removed until oligosaccharides liberated by extracellular digestion have been assimilated by the cells. The results support a model of extracellular digestion of hemicellulose to oligosaccharides that are then transported to the cytoplasm for further digestion by intracellular enzymes.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Cytosolic Oligosaccharide-Degrading Proteome of Butyrivibrio Proteoclasticus

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…The overall fi nding of this experiment established (1) the 17.5-fold abundance of endo-1,4-β-xylanase Xyn10B suggesting its major role in hemicellulose degradation and (2) the modulation of the secretion of hemicellulose-degrading enzymes and ATP-dependent sugar uptake systems in B. proteoclasticus advocating the notion about this organism as a major contributor to polysaccharide degradation in the rumen. In another study, Bond et al ( 2012 ) studied membrane proteins of the rumen bacterium Butyrivibrio proteoclasticus and identifi ed 13 membrane proteins predicted to function as carbohydrate transporters when cells were grown with fructose or xylan as the sole substrate. In another study to get insights into Escherichia coli O157:H7 (O157), survival mechanisms in the bovine rumen, growth characteristics and proteome of O157 cultured in rumen fl uid (RF; pH 6.0-7.2 and low volatile fatty acid content) obtained from rumen-fi stulated cattle fed lowprotein-content 'maintenance diet' under diverse in vitro conditions were characterized (Kudva et al 2014 ).…”

Section: Rumen Manipulation Using Proteomic Approachesmentioning

confidence: 99%

‘Omics’ Approaches to Understand and Manipulate Rumen Microbial Function

Shrivastava

Jain

Kumar

et al. 2015

Rumen Microbiology: From Evolution to Revolution

View full text Add to dashboard Cite

Diverse populations of rumen microorganisms in gut contribute to develop ability of breaking down fi brous foods, which are mostly unusable by humans (Owens FN, Goetsch AL (1988) Ruminal fermentation. In: Church DC (ed) The ruminant animal, digestive physiology and nutrition. Prentice-Hall, Englewood Cliffs, p 160). Rumen is having a larger population of microorganisms, more than a trillion organisms and wide diversity (hundreds of species and thousands of subspecies), per ounce of rumen contents (Xu et al., J Anim Sci 85:1024-1029, 2007. There are various traditional approaches through which overall performance of the rumen has been attempted to improve, e.g. plant secondary metabolites, microbial feed additives, chemical feed additives, selective stimulation of benefi cial rumen microbes and selective inhibition of harmful rumen microbes. In spite of these, nowadays various new approaches are being used to improve our understanding of the relationships among the various rumen microorganisms and towards how they interact with their hosts (Chaucheyras-Durand and Ossa, Prof Anim Sci 30:1-12, 2014). To better characterize species in the rumen, new advanced technological aids such as gene sequencing and study of gene (genomics), protein (proteomics) and metabolite (metabolomics) expression are being frequently used. This chapter will majorly emphasize on recent tools used in exploring the diversity of rumen.

show abstract

Moringa leaf meal exerts growth benefits in small ruminants through modulating the gastrointestinal microbiome

Nehra,

Harshini,

Shukla

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

This study investigated the impact of feeding 17% moringa leaf meal (MLM) on the ruminal and fecal microbial composition and body weight gain (BWG) performance of lambs ( Ovis aries ) and kids ( Capra hircus ). A total of n = 28 lambs ( n = 14, no-moringa, n = 14, 17% moringa) and 24 kids ( n = 12, no-moringa, n = 12, 17% moringa) were involved in the experiment and body weight was recorded fortnightly. Metagenomic shotgun sequencing was performed on 28, 22, and 26 ruminal solid, liquid fraction, and fecal samples from lambs, and 23, 22, and 23 samples from kids. Moringa supplementation significantly increased BWG in lambs (21.09 ± 0.78 to 26.12 ± 0.81 kg) and kids (14.60 ± 1.29 to 18.28 ± 1.09 kg) ( p -value ≤ 0.01). Microbiome analysis revealed an elevated Firmicutes:Bacteroidetes ratio in the moringa diet group. Moringa-fed animals exhibited increased microbial genera associated with volatile fatty acids (VFAs) production ( Prevotella, Anaerovibrio, Lachnospiraceae, Butyrivibrio, Christensenella ) and starch and fiber digesters ( Proteobacteria, Ruminococcus ). The increase in the bacterial genus Sharpea suggested possible methane reduction and decreased proportion of pathogens, Aliarcobacter _ ID28198 , Campylobacter _ ID194 and Campylobacter _ ID1660076 suggest health benefits. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated significant alterations in microbial gene pool and metabolic pathways related to carbohydrate, protein, lipid and energy metabolism, indicating potential improvements in animal health. Overall, moringa feeding showed higher energy recovery, improved growth, and potential benefits in methane reduction and reduced pathogenic bacteria. Supplementary Information The online version contains supplementary material available at 10.1007/s00253-024-13265-5.

show abstract

Carbohydrate transporting membrane proteins of the rumen bacterium, Butyrivibrio proteoclasticus

Cited by 17 publications

References 26 publications

The Cytosolic Oligosaccharide-Degrading Proteome of Butyrivibrio Proteoclasticus

The Cytosolic Oligosaccharide-Degrading Proteome of Butyrivibrio Proteoclasticus

‘Omics’ Approaches to Understand and Manipulate Rumen Microbial Function

Moringa leaf meal exerts growth benefits in small ruminants through modulating the gastrointestinal microbiome

Contact Info

Product

Resources

About