Moringa leaf meal exerts growth benefits in small ruminants through modulating the gastrointestinal microbiome

Abstract

This study investigated the impact of feeding 17% moringa leaf meal (MLM) on the ruminal and fecal microbial composition and body weight gain (BWG) performance of lambs ( Ovis aries ) and kids ( Capra hircus ). A total of n = 28 lambs ( n = 14, no-moringa, n = 14, 17% moringa) and 24 kids ( n = 12, no-moringa, n = 12, 17% moringa) were involved in the experiment and body weight was recorded fortnightly. Metagenomic shotgun sequencing was performed on 28, 22, and 26 ruminal solid, liquid fraction, and fecal samples from lambs, and 23, 22, and 23 samples from kids. Moringa supplementation significantly increased BWG in lambs (21.09 ± 0.78 to 26.12 ± 0.81 kg) and kids (14.60 ± 1.29 to 18.28 ± 1.09 kg) ( p -value ≤ 0.01). Microbiome analysis revealed an elevated Firmicutes:Bacteroidetes ratio in the moringa diet group. Moringa-fed animals exhibited increased microbial genera associated with volatile fatty acids (VFAs) production ( Prevotella, Anaerovibrio, Lachnospiraceae, Butyrivibrio, Christensenella ) and starch and fiber digesters ( Proteobacteria, Ruminococcus ). The increase in the bacterial genus Sharpea suggested possible methane reduction and decreased proportion of pathogens, Aliarcobacter _ ID28198 , Campylobacter _ ID194 and Campylobacter _ ID1660076 suggest health benefits. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated significant alterations in microbial gene pool and metabolic pathways related to carbohydrate, protein, lipid and energy metabolism, indicating potential improvements in animal health. Overall, moringa feeding showed higher energy recovery, improved growth, and potential benefits in methane reduction and reduced pathogenic bacteria. Supplementary Information The online version contains supplementary material available at 10.1007/s00253-024-13265-5.