Capturing intracellular oncogenic microRNAs with self-assembled DNA nanostructures for microRNA-based cancer therapy

Abstract

Aberrantly overexpressed oncogenic microRNAs (miRNAs, miRs) are excellent targets for therapeutic interventions.

In Silico and In Cell Analysis of Openable DNA Nanocages for miRNA Silencing

“…This result indicates that the here-described H4-NCs can be applied as therapeutic nanoparticles, either for release of a useful payload or for short RNA sequestering. In line, a minimal DNA cage was designed for encapsulation of small RNAs and conditional release in the presence of selected trigger strands [26], and DNA nanotubes, carrying multiple DNA segments, were proposed for capturing overexpressed oncogenic miRNAs [9]. DNA-based nanostructures, mainly based on a tetrahedral geometry, were implemented for miRNA biosensing, using electrochemical, optical, and microscopic strategies as sensing approaches [17].…”

“…DNA strands can be modified with cellular recognition signals, such as folate, transferrin, or aptamers, which permit the assembly of functionalized DNA-based nanostructures (DNS) useful for selective targeting into cells through receptor-mediated mechanism [3][4][5]. Due to their intrinsic biocompatible, nontoxic, and stable properties, DNS have been extensively investigated for various biomedical applications, such as drug delivery, cellular biosensing, and in vivo imaging [4][5][6][7][8], and, more recently, in gene silencing and RNA anticancer therapy [9,10]. Different shape-changing structural modules can be integrated in the DNS, allowing input-induced conformational changes.…”

Accurate cancer cell identification and microRNA silencing induced therapy using tailored DNA tetrahedron nanostructures

“…[19][20][21] It can induce sequence-specic inhibition of oncogene expression or translation through the delivery of antagomirs to cancer cells, which makes it possess advantages of high specicity, improved safety, high efficacy and unrestricted choice of targets. 22,23 For example, leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) is a novel gastric cancer marker, and silencing its expression with antagomirs could efficiently inhibit cancer angiogenesis. 24 miR-10b was overexpressed in metastatic breast tumor patients, and silencing of miR-10b with antagomirs could signicantly decrease miR-10b levels and suppress breast cancer metastasis.…”

Stimuli‐Responsive DNA Self‐Assembly: From Principles to Applications

“…DNA nanoassemblies have long been recognized as ideal carriers for drug delivery and theranostics, benefitting from their molecular programmabilitya nd the ease of chemical modification. [6,[107][108][109][110][111][112][113][114] Here, the stimuli-responsiveness of dynamic DNA assemblies is an important plus to allow smart, microenviron- ment-targeted actuations toward medicala pplications. Accordingly,m ultiple functions including sensing, targeting, imaging, and therapy can be integrated into as ingle entity towardp reviously unrealizable, multiplexed, and synergistic functions.…”