Accurate cancer cell identification and microRNA silencing induced therapy using tailored DNA tetrahedron nanostructures

Su, Juan; Wu, Fengjie; Xia, Hongping; Wu, Yafeng; Liu, Songqin

doi:10.1039/c9sc04823e

Cited by 94 publications

(57 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cell proliferation assays indicate a reduction in cell viability of 51% and 53% in HeLa and IGROV1, respectively. In line, similar effects have been obtained using other DNS as vehicles for the release of anti-miR-21 sequences or for direct oncomiRs-silencing activity [39][40][41] .…”

Section: Combined Treatment With Fol-mir21-ncs and Doxorubicinsupporting

confidence: 74%

Combined and selective miR-21 silencing and doxorubicin delivery in cancer cells using tailored DNA nanostructures

et al. 2021

View full text Add to dashboard Cite

MicroRNAs play an important role in tumorigenesis and, among them, miR-21 is found to be aberrantly up-regulated in various tumors. The tumor-associated antigen, folate receptor alpha is a GPI-membrane protein overexpressed in many malignant tumors of epithelial origin, including ovarian and cervical cancers. Covalently bound octahedral DNA nanocages were functionalized with folate molecules and utilized as scaffolds to engineer four sequestering units with a miR-21 complementary sequence for obtaining biocompatible Fol-miR21-NC non-toxic nanostructures, to be able to selectively recognize folate receptor alpha-overexpressing cancer cells and sequester the oncogenic miR-21. qPCR assays showed that Fol-miR21-NCs reduce the miR-21 expression up to 80% in cancer cells in the first 2 days of treatment. Functional assays demonstrated that miR-21 sequestering leads to up-regulation of miR-21 tumor suppressor targets (i.e., PTEN and Pdcd4), reduction in cancer cell migration, reduction in proliferation, and increase in cell death. Fol-miR21-NCs can be efficiently loaded with the chemotherapeutic agent doxorubicin. Co-delivery of anti-miR-21 and doxorubicin showed additive cytotoxic effects on tumor cells, paving the way for their use as selective nucleic acid drugs.

show abstract

Section: Combined Treatment With Fol-mir21-ncs and Doxorubicinsupporting

confidence: 74%

Combined and selective miR-21 silencing and doxorubicin delivery in cancer cells using tailored DNA nanostructures

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The MCF-7 cells include the miRNA-21 and miRNA-155 as biomarkers, 57 and the HepG2 cells overexpress miRNA-21. 58 Accordingly, the different cells were treated with the tetrahedra composite shown in Scheme 1 that includes three quenched fluorophores, FAM, Cy5, and ROX. Figure 4 , entry I, shows the bright-field image and confocal fluorescence microscopy images of the MCF-7 cell recorded at the excitation wavelengths of the different fluorophores associated with the tetrahedra.…”

Section: Resultsmentioning

confidence: 99%

DNA Tetrahedra Modules as Versatile Optical Sensing Platforms for Multiplexed Analysis of miRNAs, Endonucleases, and Aptamer–Ligand Complexes

et al. 2020

View full text Add to dashboard Cite

The sensing modules for analyzing miRNAs or the endonucleases consist of tetrahedra functionalized with three different fluorophore–quencher pairs in spatially quenched configurations and hairpin units acting as recognition elements for the analytes. Three different miRNAs (miRNA-21, miRNA-221, and miRNA-155) or three different endonucleases (Nt.BbvCI, Eco RI, and Hin dIII) uncage the respective hairpins, leading to the switched-on fluorescence of the respective fluorophores and to the multiplex detection of the respective analytes. In addition, a tetrahedron module for the multiplexed analysis of aptamer ligand complexes (ligands = ATP, thrombin, VEGF) is introduced. The module includes edges modified with three spatially separated fluorophore–quencher pairs that were stretched by the respective aptamer strands to yield a switched-on fluorescent state. Formation of the respective aptamer ligands reconfigures the edges into fluorophore-quenched caged-hairpin structures that enable the multiplexed analysis of the aptamer–ligand complexes. The facile permeation of the tetrahedra structures into cells is used for the imaging of MCF-7 and HepG2 cancer cells and their discrimination from normal epithelial MCF-10A breast cells.

show abstract

“…[ 40–42 ] The unique features of DNA tetrahedra include their structural rigidity, the ability to control their sizes, [ 43,44 ] and the feasibility to dictate the spatial tethering of nucleic acids to the edges or corners of the tetrahedra. [ 45–47 ] Different applications of DNA tetrahedra were reported, including their use as functional units for sensing, [ 48,49 ] logic gate operations, [ 50 ] intracellular imaging, [ 51–54 ] delivery of therapeutic agents, [ 55–57 ] and the assembly of chiral building blocks for nanofabrication and chiroplasmonic. [ 58 ]…”

Section: Introductionmentioning

confidence: 99%

Triggered Dimerization and Trimerization of DNA Tetrahedra for Multiplexed miRNA Detection and Imaging of Cancer Cells

Zhou

Fan

Wang

et al. 2021

Small

View full text Add to dashboard Cite

The reversible and switchable triggered reconfiguration of tetrahedra nanostructures from monomer tetrahedra structures into dimer or trimer structures is introduced. The triggered bridging of monomer tetrahedra by K+‐ion‐stabilized G‐quadruplexes or T‐A•T triplexes leads to dimer or trimer tetrahedra structures that are separated by crown ether or basic pH conditions, respectively. The signal‐triggered dimerization/trimerization of DNA tetrahedra structures is used to develop multiplexed miRNA‐sensing platforms, and the tetrahedra mixture is used for intracellular sensing and imaging of miRNAs.

show abstract

Accurate cancer cell identification and microRNA silencing induced therapy using tailored DNA tetrahedron nanostructures

Abstract: DNA tetrahedron nanostructures (DTNSs) were prepared to improve the accuracy of cancer diagnosis through real-time monitoring and imaging three intracellular miRNAs, as well as to realize cancer therapy induced by miRNA silencing.

Cited by 94 publications

References 46 publications

Combined and selective miR-21 silencing and doxorubicin delivery in cancer cells using tailored DNA nanostructures

Combined and selective miR-21 silencing and doxorubicin delivery in cancer cells using tailored DNA nanostructures

DNA Tetrahedra Modules as Versatile Optical Sensing Platforms for Multiplexed Analysis of miRNAs, Endonucleases, and Aptamer–Ligand Complexes

Triggered Dimerization and Trimerization of DNA Tetrahedra for Multiplexed miRNA Detection and Imaging of Cancer Cells

Contact Info

Product

Resources

About