Capillary zone electrophoresis separations of enantiomers present in complex ionic matrices with on-line isotachophoretic sample pretreatment

Column Coupling Electrophoresis in Biomedical Analysis

“…, with permission. The principle of this hyphenated technique consists from well-defined preconcentration (concentration LODs could be reduced by a factor of 10 3 when compared to conventional single column CZE) and preseparation (up to 99% or even more interfering compounds can be isolated (Danková et al, 1999)) of trace analytes in the first, wider, capillary (isotachophoretic step) and subsequently a cut of important analytes accompanied with a segment of the matrix, leading or terminator enters the second, narrower, capillary for the final separation by CZE (Fig. 2, Fig.3).…”

Separations of enantiomers by preparative capillary isotachophoresis

“…Recent reviews dealing with the electroseparations of enantiomers [11±15], however, show that the use of ITP in the analysis of enantiomers is, in general, scarce. On the other hand, ITP was shown to have apparent analytical advantages in the separations of enantiomers present in complex biological matrices when employed as an on-line sample pretreatment technique in the column-coupling combination with CZE [20]. These advantages can be ascribed to the fact that in this combination ITP not only concentrates the enantiomers but also eliminates disturbing matrix constituents from the CE separation compartment (sample clean-up) before a final CZE separation.…”

The Effects of Micelles Standing Time and Concentration on the Peak Splitting in MEKC

“…Some other researchers argued that peak splitting phenomenon is caused by the stable products of chemical reaction between samples and surfactant [12]. The peak-splitting phenomenon has a detrimental effect on analyses in MEKC in some cases, so it is important to find out effective ways to avoid peak splitting phenomenon [13].…”