Column Coupling Electrophoresis in Biomedical Analysis

Mikuš, Peter; Maráková, Katarína

doi:10.5772/21895

Cited by 1 publication

(2 citation statements)

References 131 publications

(166 reference statements)

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“…ITP optimization. ITP is a powerful technique for the preconcentration and preseparation of the complex samples [ 16 , 38 , 39 ]. The composition of leading (LE) and terminating (TE) electrolytes was optimized in order to obtain a proper sample preconcentration and preseparation with the possibility of well-defined selection of the zone of interest (i.e., ITP zone in which 5-HT migrates) from the isotachophoretic sample profile.…”

Section: Resultsmentioning

confidence: 99%

“…An advanced column-coupling arrangement employing wide-bore capillaries in a modular hydrodynamically closed separation system can be a very effective approach offering additional benefits to the conventional single column CE technique and overcoming some of its disadvantages [ 16 , 17 , 18 ]. Two separate stages can provide a sample preparation and analytical separation of an original (i.e., unpretreated) sample in one compact experiment.…”

Section: Introductionmentioning

confidence: 99%

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Two-Dimensional Capillary Electrophoresis with On-Line Sample Preparation and Cyclodextrin Separation Environment for Direct Determination of Serotonin in Human Urine

2017

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An advanced two-dimensional capillary electrophoresis method, based on on-line combination of capillary isotachophoresis and capillary zone electrophoresis with cyclodextrin additive in background electrolyte, was developed for effective determination of serotonin in human urine. Hydrodynamically closed separation system and large bore capillaries (300–800 µm) were chosen for the possibility to enhance the sample load capacity, and, by that, to decrease limit of detection. Isotachophoresis served for the sample preseparation, defined elimination of sample matrix constituents (sample clean up), and preconcentration of the analyte. Cyclodextrin separation environment enhanced separation selectivity of capillary zone electrophoresis. In this way, serotonin could be successfully separated from the rest of the sample matrix constituents migrating in capillary zone electrophoresis step so that human urine could be directly (i.e., without any external sample preparation) injected into the analyzer. The proposed method was successfully validated, showing favorable parameters of sensitivity (limit of detection for serotonin was 2.32 ng·mL−1), linearity (regression coefficient higher than 0.99), precision (repeatability of the migration time and peak area were in the range of 0.02–1.17% and 5.25–7.88%, respectively), and recovery (ranging in the interval of 90.0–93.6%). The developed method was applied for the assay of the human urine samples obtained from healthy volunteers. The determined concentrations of serotonin in such samples were in the range of 12.4–491.2 ng·mL−1 that was in good agreement with literature data. This advanced method represents a highly effective, reliable, and low-cost alternative for the routine determination of serotonin as a biomarker in human urine.

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Section: Resultsmentioning

confidence: 99%