Capillary electrophoresis with inductively coupled plasma‐mass spectrometric and electrospray time of flight mass spectrometric detection for the determination of arsenic species in fish samples

Abstract

CE was coupled to inductively coupled plasma MS (ICP-MS) and ESI-MS to identify and quantify the arsenic species arsenobetaine (AsB), arsenite (As(III)), arsenate (As(V)), and dimethylarsinic acid (DMA). A GC-flame ionization detector (FID)-based German standard method and ICP-MS were used for validation of the data obtained for arsenobetaine and total arsenic, respectively. LODs obtained with the CE-ESI-TOF-MS method were 1.0x10(-7) M for AsB, 5.0x10(-7) M for DMA, and 1.0x10(-6) M for As(III) and As(V). For the CE-ICP-MS method, LODs were 8.5x10(-8) M for AsB, 9.5x10(-8) M for DMA, 9.3x10(-8) M for As(III), and 6.2x10(-8) M for As(V). While CE-ICP-MS provided high sensitivity and better reproducibility for quantitative measurements, CE-ESI-MS with a TOF mass analyzer proved to be valuable for species identification. With this setup, fish samples were prepared and analyzed and the obtained data were successfully validated with the independent methods.

Speciation analysis of arsenic compounds by capillary electrophoresis on-line coupled with inductively coupled plasma mass spectrometry using a novel interface

“…The LODs for various arsenic species were in the range of 0.9-3.0 ng g −1 , corresponding to absolute detection limits in the range of 19-65 fg of As based on a 21.5 nL sample injection (Table 2). Compared to the other CE-ICP-MS methods [10,23,26,32,36], our method could simultaneously separate more arsenic compounds with higher separation efficiency than other methods. The detection limits were comparable or even better than those of the reported methods.…”

“…For the coupling of CE with ICP-MS, the interface used in this study was fabricated by a CE-ESI-MS sprayer kit (G1607A, Agilent, USA), which has previously been used to couple CE with ESI-MS [31,32]. The sprayer consists of a concentrically shaped stainless steel capillary surrounded by an outer stainless steel tube and was used as the nebulizer of the ICP-MS. As shown in Fig.…”

“…The effluent from the capillary would be directly nebulized at the sprayer tip which ensured that no dead volume existed before nebulization. This interface has been reported for coupling CE-ESI-MS system [31] and CE-ESI-TOF-MS system [32], but to the best of our knowledge it has not yet been applied to couple CE with ICP-MS.…”

Recent food safety and food quality applications of CE‐MS

“…Even though, some other interesting applications like the analysis of biogenic amines [27,28], alkaloids [29][30][31], antidepressants [32,33], disinfection byproducts [34], products formed during the processing or cooking of food, such as acrylamides [35], speciation analysis [36][37][38], chemical warfare agent degradation products [39], transgenic foods [30,40] and toxic oligopeptides [41] have also been carried out in this field by CE-MS. Table 1 shows these recent examples of the application of CE-MS.…”

Ionic liquid based carrier mediated hollow fiber liquid liquid liquid microextraction combined with HPLC-ICP-MS for the speciation of phenylarsenic compounds in chicken and feed samples

“…4 The most commonly used separation technique is high performance liquid chromatography (HPLC), 1,5,6 while gas chromatography (GC) 7 and capillary electrophoresis (CE) 8 are less used because GC requires a derivatization step to convert non-volatile arsenic compounds into their volatile derivatives prior to the separation 9 and CE lacks sensitivity and selectivity for realworld samples with a complex matrix. 11 In contrast, RP-HPLC has the potential to simultaneously separate both charged and uncharged analytes with the addition of appropriate ion pair reagents. IEC is normally used in separations of charged analytes, while anions and cations cannot be separated within one run.…”