Capillary Electrophoresis of Free Fatty Acids by Indirect Ultraviolet Detection: Application to the Classification of Vegetable Oils According to Their Botanical Origin

Vergara-Barberán, María; Escrig-Doménech, Aarón; Lerma‐García, María Jesús; Simó‐Alfonso, Ernesto Francisco; Herrero‐Martínez, José Manuel

doi:10.1021/jf202798c

Cited by 27 publications

(29 citation statements)

References 30 publications

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“…A number of methods have been reported. These include GC , LC , supercritical fluid chromatography , CE , and MEKC in conjunction with a variety of detectors. A serious drawback for most of the procedures is the requirement of derivatization, which is tedious and time consuming.…”

Section: Introductionmentioning

confidence: 99%

Rapid separation of fatty acids using a poly(vinyl alcohol) coated capillary in nonaqueous capillary electrophoresis with contactless conductivity detection

2013

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The determination of fatty acids by nonaqueous CZE with capacitively coupled contactless conductivity detection was investigated. A new deoxycholate-based BGE, which had previously been found to give significantly improved baseline stability in the determination of lipophilic organic ammonium ions, was found to be similarly beneficial for the determination of the anions. The use of a PVA-coated capillary was required for suppression of the EOF and to obtain well reproducible results. The complete separation of 12 fatty acids could be achieved with 10 mM DOC in methanol within 6 min under optimized conditions. The PVA-coated capillary demonstrated outstanding stability over 300 runs with no sign of depletion of the PVA layer. Method validation showed a good linearity range from 0.75 to 25 μM with correlation coefficients between 0.9949 and 0.9979. The LOD was determined as 0.5 μM for all fatty acids. The developed approach was successfully demonstrated for the separation of free fatty acids in commercial and home-made edible oil.

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Section: Introductionmentioning

confidence: 99%

Rapid separation of fatty acids using a poly(vinyl alcohol) coated capillary in nonaqueous capillary electrophoresis with contactless conductivity detection

2013

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“…The authors were able to detect FFAs within the M range in a coco oil extract. Later, a similar study was conducted using dimethyl-␤-CD for the separation of hydrolyzed crude vegetable oils [4], in which it was possible to separate C18:0, C18:1, C18:2, C18:3 ⌬ cis 6,9,12 and C18:3 ⌬ cis 6,9,15 FFAs in less than 15 min at base peak resolution. To achieve this level of performance, it was required that the injected sample volume does not exceed 4 nL.…”

Section: Uv Detectionmentioning

confidence: 99%

A digest of capillary electrophoretic methods applied to lipid analyzes

Poinsot

Meang

et al. 2018

Electrophoresis

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Lipids are naturally occurring organic compounds that can be classified into a number of types based on their solubility in nonpolar organic solvents, and are generally insoluble in water. The great structural variety of these various types of lipids has led them to be components of many different biological substances such as oils, waxes, cellular membranes, tissues and biological fluids. The use of capillary electrophoresis (CE) for the study of lipids during the past 30 years has been relatively rare when compared to its use for other classes of biomolecules, primarily due to their insolubility in water. However, a number of interesting studies have been conducted, and as part of this review, we will present the different approaches that were used, which mainly consist of micellar kinetic chromatography and non-aqueous CE. The main advantages of the use of these techniques compared to GC is the very simple sample preparation that is required and, compared to LC, the very robust and quick separations that can be obtained. In this review, we present the various methods that have been reported in the literature that have been used for the study of fatty acids, phospholipids, glycerides, eicosanoids and sterols, with the inclusion of various tables presenting descriptions of the CE methods used as well as the methods of detection, including UV absorbance, fluorescence, mass spectrometry, and conductivity. This review aims to demonstrate that CE can be easily used for the analysis of lipids.

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“…10,11 In ancient times, the homemade production of castor oil was disseminated by Brazilian countryside families, which had low financial resources and difficulty in the access of health care, for its anti-inflammatory, antioxidant and laxative properties. 12 Several analytical techniques can be applied in analysis and certification of vegetable oils such as liquid chromatography, 13,14 gas chromatography, 15-17 capillary electrophoresis, 18,19 nuclear magnetic resonance, [20][21][22] and near-infrared spectroscopy. 23,24 However, in the last decade vibrational spectroscopies have been presented as an alternative, since the analysis is fast and usually the sample preparation is very simple.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of the Lipid Profiles of Oils from Kernels of Peanut, Babassu, Coconut, Castor and Grape by GC-FID and Raman Spectroscopy

Martini¹,

Porto²,

Oliveira³

et al. 2017

J. Braz. Chem. Soc.

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The characterization of the lipid profiles of oils extracted from the kernel of seeds and nuts of peanut (Arachis hypogaea), babassu (Attalea speciosa), coconut (Cocos nucifera), castor (Ricinus communis) and grape (Vitis vinifera) was performed by using Raman spectroscopy and gas chromatography with flame ionization detector (GC-FID). Chromatographic analyses showed that coconut and babassu oils are composed essentially by saturated fatty acids (SFA), whereas the others are composed mainly by unsaturated fatty acids (UFA). The comparison of commercial and homemade castor oils showed the former have a lower level of ricinoleic acid at ca. 58%. Spectroscopic analyses of three distinct castor oils showed their profiles could be differentiated by marker features ascribed to carbonyl modes which were correlated with storage time. The comparison between the Raman spectra of standard samples of fatty acids with the samples of oils allowed the assignment of the spectroscopic features and the characterization of marker bands for the degree of unsaturation.

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Capillary Electrophoresis of Free Fatty Acids by Indirect Ultraviolet Detection: Application to the Classification of Vegetable Oils According to Their Botanical Origin

Cited by 27 publications

References 30 publications

Rapid separation of fatty acids using a poly(vinyl alcohol) coated capillary in nonaqueous capillary electrophoresis with contactless conductivity detection

Rapid separation of fatty acids using a poly(vinyl alcohol) coated capillary in nonaqueous capillary electrophoresis with contactless conductivity detection

A digest of capillary electrophoretic methods applied to lipid analyzes

Comparative Study of the Lipid Profiles of Oils from Kernels of Peanut, Babassu, Coconut, Castor and Grape by GC-FID and Raman Spectroscopy

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