Capillary electrophoresis and off-line capillary electrophoresis–electrospray ionization quadrupole time-of-flight tandem mass spectrometry of carbohydrates

Zamfir, Alina D.; König, Simone; Althoff, J.; Peter-Katalinc, Jasna

doi:10.1016/s0021-9673(00)00411-8

Cited by 32 publications

(28 citation statements)

References 11 publications

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“…In lower abundance, the Ser-linked Neu5Ac 2 Hex 2 HexNAc 2 was detected as a doubly charged ion at m/z 707.75. Although previously [53] the off-line CE/MS was reported to require rather high amounts of glycopeptide sample for detection, the final concentration of the fraction was here approximately 3 pmol/mL. In the case of ammonium formate, the 75 nL/min flow rate exhibited by the NanoMate chip system yielded a fair S/N by only 30 scans of signal accumulation (Fig.…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 81%

“…In the case of ammonium formate, the 75 nL/min flow rate exhibited by the NanoMate chip system yielded a fair S/N by only 30 scans of signal accumulation (Fig. 11) under the sample consumption of approximately 0.4 pmol, which is a superior sensitivity in comparison with the capillary-based experiments [53,54]. Due to the high spray stability, the MS/MS investigation of a low abundant doubly charged ion at m/z 707.75 attributed to Neu5Ac 2 Hex 2 HexNAc 2 -Ser was greatly enhanced (Fig.…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 96%

“…For this reason, the off-line coupling of CE to (2)nanoESI-QTOF-MS has been lately intensively developed and implemented for glycoconjugate analysis. Its potential for separation, screening, and structural elucidation of different types of glycoconjugates, such as glycosaminoglycans [51,52], O-glycosylated peptides [53], and gangliosides [54], was demonstrated in detail. However, using the CE instrument as a fraction collector, the sample concentration is lowered because sample reservoirs contain electrolyte, so that volumes of a few nanoliters are diluted into tens-of-microliters.…”

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

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Chip electrospray mass spectrometry for carbohydrate analysis

Zamfir¹,

Bîndilă²,

Lion³

et al. 2005

Electrophoresis

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Currently two types of chip systems are used in conjunction with MS: out-of-plane devices, where hundreds of nozzles, nanospray emitters are integrated onto a single silicon substrate from which electrospray is established perpendicular to the substrate, and planar microchips, embedding a microchannel at the end of which electrospray is generated in-plane, on the edge of the microchip. In the last two years, carbohydrate research greatly benefited from the introduction and implementation of the chip-based MS. In two laboratories the advantages of the chip electrospray in terms of ionization efficiency, sensitivity, reproducibility, quality of data in combination with high mass accuracy, and resolution of detection were systematically explored for several carbohydrate classes: O-and N-glycopeptides, oligosaccharides, gangliosides and glycoprotein-derived O-and N-glycans, and glycopeptides. The current state-of-the-art in interfacing the chip electrospray devices to high-performance MS for carbohydrate analysis, and the particular requirements for method optimization in both positive and negative ion modes are reviewed here. The recent applications of these miniaturized devices and their general potential for glycomic-based surveys are highlighted.

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Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 81%

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 96%

Section: Complex Biological Mixtures Of Glycopeptidesmentioning

confidence: 99%

See 1 more Smart Citation

Chip electrospray mass spectrometry for carbohydrate analysis

Zamfir¹,

Bîndilă²,

Lion³

et al. 2005

Electrophoresis

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“…The use of powerful mass spectrometers can partially counterbalance this drawback, as demonstrated by Zamfir and co-workers [27,36,64], who successfully characterized a number of glycoconjugates by CE off-line coupled with MS.…”

Section: Analysis Of Glycans From Glycoproteinsmentioning

confidence: 99%

“…To date, the main articles regarding the CE-MS of saccharidic compounds found in literature include the analysis of (i) standard mixtures [42,[47][48][49][50][51][52]; (ii) carbohydrates in nutrition [19,[53][54][55][56][57][58][59]; (iii) glycans from glycoproteins [60][61][62][63][64][65]; (iv) carbohydrates in drug development [45,[66][67][68][69][70][71][72][73][74][75][76][77][78][79][80][81].…”

Section: Introductionmentioning

confidence: 99%

Overview on advances in capillary electrophoresis‐mass spectrometry of carbohydrates: A tabulated review

et al. 2006

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Overview on advances in capillary electrophoresis-mass spectrometry of carbohydrates: A tabulated reviewThe increasing interest for carbohydrates as holder of essential bioinformations has boosted their full characterization through analytical techniques. The intent of this review is to summarize the recent trends regarding on-line and off-line CE-MS coupling for carbohydrate analysis. A statistical survey on the articles that use derivatizing agents as well as on the analyzer and type of instrument coupling (i.e. on-or off-line) is depicted. From a general overview it can be concluded that, whereas derivatization might be useful for the detection of neutral carbohydrates improving separation selectivity with volatile buffers and increasing sensitivity of the MS detection, relatively few works with derivatized carbohydrates were found; this was noticed in particular for glycosides and saccharides carrying ionizable groups, which are normally analyzed without any chemical modification. The most applied coupling is the on-line sheath-liquid interface; for online applications, ESI is the sole source used, whilst the most common analyzer is the IT. MS n is often exploited, as fragmentation increases the achieved structural information. CE-MS turned out to be mainly used for the analysis of carbohydrates in drug development (i.e. study of oligosaccharides from pathogens, carbohydrate-based drugs and drug metabolites), in nutrition and for characterization of glycans from glycoproteins. The reader will find elucidating tables regarding these recent CE-MS applications, including the main information on the analysis conditions. Comments are meant to help the immediate focus on the usefulness of the analytical technique and predict the difficulties found during analysis and, in case, their overcoming.

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