Capillary electrochromatographic separation of aromatic amino acids possessing peptides using porphyrin derivatives as the inner wall modifiers

Charvátová, Jana; Král, Vladimı́r; Deyl, Z.

doi:10.1016/s1570-0232(02)00051-x

Cited by 21 publications

(13 citation statements)

References 19 publications

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“…The OT-CEC mode has been used to study the interactions of oligopeptides, diglycine and triglycine, aromatic amino acids possessing peptides, and selected aliphatic and aromatic amino acids with (metallo)porphyrin derivatives immobilized by physical adsorption to the FS capillary wall [132,133]. OT-CEC with covalently immobilized DNA oligonucleotides containing a thrombin-binding aptamer that forms a biplanar G-quartet structure and an oligonucleotide that forms a 4-plane G-quartet structure was capable to separate isomeric dipeptides Trp-Arg and Arg-Trp [134].…”

Section: Electrochromatographymentioning

confidence: 99%

Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

Kasicka

2003

Electrophoresis

106

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An overview of the recent developments in the applications of high-performance capillary electromigration methods, namely zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides is presented. New approaches to the theoretical description and experimental verification of the electromigration behavior of peptides and the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample treatment, and suppression of adsorption, are discussed, and new developments in individual separation modes and new designs of detection systems applied to peptide separations are shown. Several types of applications of capillary electromigration methods to peptide analysis are presented: quality control and purity tests, determination in biomatrices, monitoring of physical and chemical changes and enzymatic conversions, amino acid and sequence analysis, and peptide mapping. The examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Electrochromatographymentioning

confidence: 99%

Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

Kasicka

2003

Electrophoresis

106

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“…Charvátová et al [72] used porphyrin derivates as wall modifier for the OT-CEC separation of aromatic peptides. The main separation mechanism was based on the ionic properties of the separated analytes.…”

Section: Peptidesmentioning

confidence: 99%

Recent applications in capillary electrochromatography

2003

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The most recent and important applications in capillary electrochromatography (CEC) are summarized, covering literature published since May 2001. A selection of new developments in stationary phases for CEC is highlighted, and enantiomeric separations and chiral stationary phases are discussed. Also, CEC applications of biological molecules, pharmaceuticals, and applications in the field of industrial and environmental analysis are summarized. For this review three modes of CEC were taken into account, i.e., packed-column CEC, CEC using monolith technology, and open-tubular CEC.

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“…Batch-to-batch reproducibility for both capillaries was excellent, as characterized by the selectivity and peak shape behavior of two basic small molecules and two proteins. Charvatova and coworkers [4,5] used porphyrin derivatives as the inner wall modi®ers to separate peptides that contain aromatic amino acids and oligopeptides. Recently, a DNA aptamer that forms a G-quartet conformation was covalently attached to a capillary surface to separate two forms of bovine b-lactoglobulin that differ by only two amino residues [6].…”

Section: Open-tubular Capillary Electrochromatography Of Peptides Andmentioning

confidence: 99%

The separation of biomolecules using capillary electrochromatography

Huang

Jin

et al. 2003

Current Opinion in Biotechnology

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Capillary electrochromatographic separation of aromatic amino acids possessing peptides using porphyrin derivatives as the inner wall modifiers

Cited by 21 publications

References 19 publications

Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

Recent applications in capillary electrochromatography

The separation of biomolecules using capillary electrochromatography

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